过敏性紫癜患儿血清常见呼吸道感染病原体特异性IgM的检测结果分析

发布时间：2018-09-06 08:28

【摘要】：目的分析过敏性紫癜患儿血清中常见呼吸道感染病原体IgM的检测结果。方法选择80例HSP患儿纳入HSP组,80例建康体检儿童纳入对照组。采用间接免疫荧光法分别检测两组血清中嗜肺军团菌、肺炎支原体、Q热立克次体、肺炎衣原体、腺病毒、呼吸道合胞病毒、甲型流感病毒、乙型流感病毒和副流感病毒的特异性IgM,免疫荧光显微镜下观察结果;采用散射比浊法检测两组血清中的链球菌溶血素(ASO)。结果 HSP组有1种或1种以上呼吸道病原体IgM阳性或ASO增高者共39例、对照组为20例,HSP组呼吸道病原体感染率为48.8%,高于对照组的25.0%(P0.05)。HSP组血清嗜肺军团菌IgM、肺炎支原体IgM、肺炎衣原体IgM、腺病毒IgM、乙型流感病毒IgM阳性及ASO阳性分别为12(15.00%)、22(27.50%)、1(1.25%)、2(2.50%)、18(22.50%)、6(7.50%)例,对照组分别为4(5.00%)、10(12.50%)、0(0)、0(0)、6(7.50%)、1(1.25%)例;HSP组嗜肺军团菌IgM、肺炎支原体IgM、乙型流感病毒IgM检出率均高于对照组(P均0.05)。两组血清中均未检出立克次体IgM、呼吸道合胞病毒IgM、甲型流感病毒IgM、副流感病毒IgM。HSP组冬季肺炎支原体IgM、乙型流感病毒IgM检出率高于对照组,秋季乙型流感病毒IgM检出率高于对照组(P均0.05)。HSP组中嗜肺军团菌IgM、肺炎支原体IgM、乙型流感病毒IgM秋冬季节检出率明显高于春夏季节(P均0.05)。结论 HSP患儿血清中多种常见呼吸道感染病原体抗体阳性,其中嗜肺军团菌、肺炎支原体、乙型流感病毒抗体检出率高于正常;呼吸道病原体感染可能与HSP的发病有关。
[Abstract]:Objective to analyze the detection results of common respiratory tract infection pathogen IgM in children with Henoch-Schonlein purpura. Methods 80 children with HSP were enrolled in HSP group and 80 children in Jiankang physical examination group were included in the control group. Indirect immunofluorescence assay was used to detect Legionella pneumophila, Mycoplasma pneumoniae Q fever Rickettsia, Chlamydia pneumoniae, adenovirus, respiratory syncytial virus, influenza A virus, respectively. Specific IgM, immunofluorescence microscopy of influenza B virus and parainfluenza virus. Detection of streptococcal hemolysin (ASO). In serum of two groups by scatter turbidimetry Results in the HSP group, there were 39 cases with one or more respiratory pathogens IgM positive or ASO increased. The infection rate of respiratory tract pathogens in the control group was 48.8%, which was higher than that in the control group (25.0%, P0.05). The positive rates of IgM and ASO were 12 (15.00%), 22 (27.50%), 2 (2.50%), 18 (22.50%) and 7.50%, respectively, in the serum of Legionella pneumoniae (IgM,), Chlamydia pneumoniae (Chlamydia pneumoniae), adenovirus type B influenza virus (IgM) and ASO (2.50%). The detection rate of IgM of Legionella pneumoniae IgM, type B virus in control group was higher than that in control group (4 (5.00%), 10 (12.50%), 0 (0) 0 (7.50%) (7.50%) and 1. 25% (1. 25%), respectively. The positive rate of IgM of Mycoplasma pneumoniae IgM, type B influenza virus in HSP2 group was higher than that in control group (all P 0. 05). No Rickettsia IgM, respiratory syncytial virus IgM, influenza A IgM, parainfluenza virus IgM.HSP was detected in the serum of the two groups. The detection rate of Mycoplasma pneumoniae IgM, B influenza virus IgM was higher than that of the control group. The detection rate of IgM of influenza B virus in autumn was higher than that of control group (P0. 05). The detection rate of Legionella pneumoniae IgM, pneumonia mycoplasma IgM, influenza virus IgM in autumn and winter was significantly higher than that in spring and summer (P 0. 05). Conclusion the positive rate of antibodies against various common respiratory tract infections in children with HSP is higher than that in normal children, including Legionella pneumophila, Mycoplasma pneumoniae and influenza B virus, and the infection of respiratory pathogens may be related to the pathogenesis of HSP.
【作者单位】：青岛大学附属医院;
【分类号】：R725.5

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