3、7型腺病毒纤毛与六邻体基因序列分析
发布时间：2018-09-13 13:16
【摘要】：1背景：2009年6月至2011年2月课题组从呼吸道感染住院患儿中共检出人腺病毒(Human adenovirus, HAdV)阳性标本105份,其中3型55例,7型40例,5型4例,1型4例,55型1例,2型1例,而2010-2011年冬,腺病毒感染率激增。由于HAdV易发生血清型间重组现象。为进一步明确是否因重组造成了HAdV的感染率激增,本研究分析比较前期临床分离的3,7型HAdV纤毛(Fiber)与六邻体(Hexon)蛋白的基因序列。 方法：从2009年6月～2011年5月,收集因呼吸道感染在重庆医科大学附属儿童医院呼吸病房住院患儿鼻咽抽吸物(Nasopharyngeal aspirates, NAPs)1780例。所有标本进行HAdV PCR扩增,腺病毒阳性者测序,基因库进行比对确定血清型。对所有HAdV-B阳性分别进行Hexon高变区(Highly variable region, HVR)及纤毛蛋白基因PCR扩增测序,并对3型和7型腺病毒进行进化树分析(Phylogeny analysis)及相似度分析(Similarity analysis)。根据已确定的腺病毒血清型进行相关临床资料分析,统计分析软件使用SPSS17.0。 结果：重庆地区引起儿童急性呼吸道感染(Acute respiratory tract infection, ARTI)的腺病毒血清型主要为3型和7型。Logestic回归分析发现,HAdV-7相对于HAdV-3更容易致重症肺炎(OR=0.089,95%CI0.021-0.375)。建立所有B组HAdV进化树,并未发现重组现象。SimPlot相似度分析发现HAdV-3和HAdV-7序列差异集中在纤毛蛋白(Fiber)的基因序列。 结论:HAdV-3和HAdV-7是引起重庆地区儿童ARTI的主要血清型,但两者引起肺炎严重度不同。基因序列分析提示纤毛蛋白序列差异可能导致HAdV-3和HAdV-7腺病毒相关肺炎严重度差异。
[Abstract]:Background: from June 2009 to February 2011, 105 human adenovirus (Human adenovirus, HAdV) positive specimens were detected from hospitalized children with respiratory tract infection, including 55 cases of type 3, 40 cases of type 7, 4 cases of type 5, 1 case of type 55, 1 case of type 2, and 1 case of type 2 in winter 2010-2011. Adenovirus infection rates have soared. HAdV is prone to serotypic recombination. In order to find out whether the infection rate of HAdV increased rapidly due to recombination, this study compared the gene sequences of the ciliated (Fiber) of type 3 HAdV and the (Hexon) protein of hexahedron. Methods: from June 2009 to May 2011, 1780 cases of nasopharyngeal aspiration (Nasopharyngeal aspirates, NAPs) were collected in respiratory ward of Children's Hospital affiliated to Chongqing Medical University. All the specimens were amplified by HAdV PCR, the adenovirus positive individuals were sequenced, and the gene bank was compared to determine the serotype. All HAdV-B positives were amplified and sequenced by Hexon hypervariable region (Highly variable region, HVR) and ciliated protein gene PCR respectively. The 3 and 7 adenovirus were analyzed by phylogenetic tree analysis (Phylogeny analysis) and similarity analysis (Similarity analysis). According to the analysis of the clinical data of adenovirus serotypes, SPSS17.0. is used in the statistical analysis software. Results: the serotypes of adenovirus in children with acute respiratory tract infection (Acute respiratory tract infection, ARTI) were mainly serotype 3 and type 7. Logestic regression analysis showed that HAdV-7 was more likely to cause severe pneumonia (OR=0.089,95%CI0.021-0.375) than that of HAdV-3. All group B HAdV phylogenetic trees were established, and no recombination phenomenon was found. SimPlot similarity analysis showed that the difference between HAdV-3 and HAdV-7 sequence was concentrated in the gene sequence of ciliated protein (Fiber). ConclusionHAdV-3 and HAdV-7 are the main serotypes of ARTI in children in Chongqing area, but the severity of pneumonia is different between them. Gene sequence analysis suggested that the difference of ciliated protein sequence might lead to the difference of severity between HAdV-3 and HAdV-7 adenovirus associated pneumonia.
【学位授予单位】：重庆医科大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R725.1
本文编号：2241280
[Abstract]:Background: from June 2009 to February 2011, 105 human adenovirus (Human adenovirus, HAdV) positive specimens were detected from hospitalized children with respiratory tract infection, including 55 cases of type 3, 40 cases of type 7, 4 cases of type 5, 1 case of type 55, 1 case of type 2, and 1 case of type 2 in winter 2010-2011. Adenovirus infection rates have soared. HAdV is prone to serotypic recombination. In order to find out whether the infection rate of HAdV increased rapidly due to recombination, this study compared the gene sequences of the ciliated (Fiber) of type 3 HAdV and the (Hexon) protein of hexahedron. Methods: from June 2009 to May 2011, 1780 cases of nasopharyngeal aspiration (Nasopharyngeal aspirates, NAPs) were collected in respiratory ward of Children's Hospital affiliated to Chongqing Medical University. All the specimens were amplified by HAdV PCR, the adenovirus positive individuals were sequenced, and the gene bank was compared to determine the serotype. All HAdV-B positives were amplified and sequenced by Hexon hypervariable region (Highly variable region, HVR) and ciliated protein gene PCR respectively. The 3 and 7 adenovirus were analyzed by phylogenetic tree analysis (Phylogeny analysis) and similarity analysis (Similarity analysis). According to the analysis of the clinical data of adenovirus serotypes, SPSS17.0. is used in the statistical analysis software. Results: the serotypes of adenovirus in children with acute respiratory tract infection (Acute respiratory tract infection, ARTI) were mainly serotype 3 and type 7. Logestic regression analysis showed that HAdV-7 was more likely to cause severe pneumonia (OR=0.089,95%CI0.021-0.375) than that of HAdV-3. All group B HAdV phylogenetic trees were established, and no recombination phenomenon was found. SimPlot similarity analysis showed that the difference between HAdV-3 and HAdV-7 sequence was concentrated in the gene sequence of ciliated protein (Fiber). ConclusionHAdV-3 and HAdV-7 are the main serotypes of ARTI in children in Chongqing area, but the severity of pneumonia is different between them. Gene sequence analysis suggested that the difference of ciliated protein sequence might lead to the difference of severity between HAdV-3 and HAdV-7 adenovirus associated pneumonia.
【学位授予单位】：重庆医科大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R725.1
【参考文献】
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1 谭耀驹;谢菲;;腺病毒分型诊断研究进展[J];广东医学;2008年01期
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