维生素A在新生期肺炎链球菌肺炎诱导哮喘发生中的作用研究
发布时间：2018-10-19 12:24
【摘要】：目的:研究维生素A(Vitamin A,VA)在新生期肺炎链球菌肺炎(streptococcus pneumoniae pneumonia,S.pp)诱导成年期哮喘发生中的作用。明确新生期S.pp促进哮喘发生是否通过影响VA水平实现及感染后VA补充是否对哮喘发病具有抑制作用;方法:将SPF级BALB/c新生雌鼠(6-8天)随机分为对照组(Con组)、肺炎链球菌肺炎组(S.pp组)及肺炎后VA补充组(S.pp+VA组),3周后(即生后4周)开始利用OVA致敏、激发建立OVA过敏性哮喘模型(OVA组、S.pp+OVA组及S.pp+VA+OVA组)。高效液相色谱法(HPLC)动态监测新生期S.pp对小鼠血清、肝脏及肺组织中VA的影响,比较Con组、OVA组、S.pp+OVA组及S.pp+VA+OVA组小鼠肺组织病理改变、气道高反应性及肺组织炎症细胞浸润、CD4+T细胞亚群水平及支气管肺泡灌洗液(Bronchoalveolar lavage fluid,BALF)中细胞因子差异,比较三组哮喘模型小鼠肺组织中STAT4总蛋白表达及磷酸化水平差异。结果:新生期S.pp引起肺部VA持续降低至感染后四周,新生期S.pp后补充VA可显著降低哮喘模型肺组织炎症细胞浸润,减轻气道高反应性及气道肺组织炎症细胞浸润,增加肺组织STAT4蛋白磷酸化水平,促进Th1、Foxp3+Treg细胞表达,抑制Th2细胞表达,而对肺组织STAT4总蛋白表达及Th17细胞水平并无显著影响。结论:VA抑制新生期S.pp诱导的哮喘发生,可能与VA影响STAT4蛋白活化,影响CD4+T细胞分化发育有关。可能通过影响STAT4活化,影响CD4+T细胞分化发育而抑制S.pp诱导的哮喘发生。
[Abstract]:Objective: To study the role of Vitamin A (VA) in the occurrence of neonatal asthma induced by Streptococcus pneumoniae (S.p.) in neonatal period. In this study, the effects of S. pp in neonatal period on asthma were determined whether by influencing VA level and VA supplementation after infection. Methods: SPF BALB/ c newborn infants (6-8 days) were randomly divided into control group (Con group). After 3 weeks (i.e. 4 weeks after birth) of Streptococcus pneumoniae pneumonia group (S. pp.) and the post-pneumonia VA supplementation group (S. pp + VA group), an allergic asthma model was established (group A, S. pp + control group and S. pp + VA + treatment group). High performance liquid chromatography (HPLC) was used to dynamically monitor the effects of S. pp on VA in serum, liver and lung tissues of mice. The expression of STAT4 and the level of phosphorylation of STAT4 in lung tissues of mice with asthma were compared. Results: After neonatal period S. pp, pulmonary VA decreased continuously to four weeks after infection. The supplementation of VA after neonatal period S. pp could significantly reduce the infiltration of inflammatory cells in lung tissue of asthma model, reduce airway hyperresponsiveness and infiltration of inflammatory cells in lung tissue, increase the phosphorylation level of STAT4 protein in lung tissue, and promote Th1. Foxp3 + Treg cells express and inhibit the expression of Th2 cells, but there is no significant effect on the expression of STAT4 total protein and the level of Th17 cells in lung tissue. Conclusion: VA inhibited the occurrence of S. pp induced asthma, which might affect the activation of STAT4 protein and affect the differentiation and development of CD4 + T cells. It is possible to suppress the occurrence of S. pp-induced asthma by influencing the activation of STAT4, which affects the differentiation and development of CD4 + T cells.
【学位授予单位】：重庆医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R722.1
本文编号：2281103
[Abstract]:Objective: To study the role of Vitamin A (VA) in the occurrence of neonatal asthma induced by Streptococcus pneumoniae (S.p.) in neonatal period. In this study, the effects of S. pp in neonatal period on asthma were determined whether by influencing VA level and VA supplementation after infection. Methods: SPF BALB/ c newborn infants (6-8 days) were randomly divided into control group (Con group). After 3 weeks (i.e. 4 weeks after birth) of Streptococcus pneumoniae pneumonia group (S. pp.) and the post-pneumonia VA supplementation group (S. pp + VA group), an allergic asthma model was established (group A, S. pp + control group and S. pp + VA + treatment group). High performance liquid chromatography (HPLC) was used to dynamically monitor the effects of S. pp on VA in serum, liver and lung tissues of mice. The expression of STAT4 and the level of phosphorylation of STAT4 in lung tissues of mice with asthma were compared. Results: After neonatal period S. pp, pulmonary VA decreased continuously to four weeks after infection. The supplementation of VA after neonatal period S. pp could significantly reduce the infiltration of inflammatory cells in lung tissue of asthma model, reduce airway hyperresponsiveness and infiltration of inflammatory cells in lung tissue, increase the phosphorylation level of STAT4 protein in lung tissue, and promote Th1. Foxp3 + Treg cells express and inhibit the expression of Th2 cells, but there is no significant effect on the expression of STAT4 total protein and the level of Th17 cells in lung tissue. Conclusion: VA inhibited the occurrence of S. pp induced asthma, which might affect the activation of STAT4 protein and affect the differentiation and development of CD4 + T cells. It is possible to suppress the occurrence of S. pp-induced asthma by influencing the activation of STAT4, which affects the differentiation and development of CD4 + T cells.
【学位授予单位】：重庆医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R722.1
【参考文献】
相关期刊论文 前1条
1 杨婷;罗征秀;符州;刘昌林;王莉佳;罗健;刘恩梅;;新生期小鼠非致死性肺炎链球菌感染模型的建立[J];中国生物制品学杂志;2012年07期
，本文编号：2281103
本文链接：https://www.wllwen.com/yixuelunwen/eklw/2281103.html
最近更新
教材专著
