寒喘宁口服液对实验性支气管哮喘豚鼠NO和TNF-a影响的实验研究

发布时间：2018-10-20 19:45

【摘要】：目的：本课题旨在利用寒喘宁口服液作用于哮喘豚鼠模型，通过观察其对哮喘豚鼠哮喘症状及支气管肺泡灌洗液中NO和TNF-a含量的影响，评价寒喘宁口服液对哮喘豚鼠炎症反应的治疗作用，为寒喘宁口服液在临床上的进一步应用提供理论依据。方法：实验选用50只幼龄雄性豚鼠在相同条件下适应性喂养1周，然后随机分组，每组10只，共5组，即正常对照组、哮喘模型组、地塞米松对照组、中药小剂量组和中药大剂量组。正常对照组以生理盐水1ml代替卵蛋白腹腔注射，剩下四组经腹腔注射10%卵白蛋白溶液1ml致敏。自注射第14天起，对哮喘模型组、地塞米松片对照组、中药小剂量组、中药大剂量组各组每天上、下午各一次将豚鼠置于4升雾缸内，用1%卵白蛋白溶液超声雾化10-20s,激发至哮喘发作，出现呼吸喘促、点头、伏地、身体颤抖等现象，提示造模成功。诱发豚鼠哮喘与灌胃给药二者同步进行，地塞米松片对照组、中药小剂量组、中药大剂量组各组于当天两次激发间隔时间内灌胃给药，每次3ml，每日2次。正常对照组每日2次以生理盐水雾化代替卵白蛋白激发。正常对照组、哮喘模型组每日2次均用3ml生理盐水灌胃,各组共灌胃2周。并观察豚鼠的精神活动、进食情况、皮毛色泽，二便及死亡等，末次给药1小时后，以1%卵白蛋白溶液雾化吸入15-20秒，观察豚鼠发生喘息等症状的的持续时间，正常组以生理盐水代替。各组除施加以上药物因素处理外，余日常饲养方法均相同，可以自由进食常规豚鼠饲料和水。通过检测豚鼠支气管肺泡灌洗液中NO和TNF-a含量水平的变化观察寒喘宁口服液的治疗效果。结果：哮喘模型组豚鼠BALF中NO、TNF-α的含量比正常对照组明显升高,而经寒喘宁口服液治疗后,BALF中NO、TNF-α的含量比哮喘模型组明显降低。结论：寒喘宁口服液能缩短哮喘的发作时间和寒症持续时间，降低支气管肺泡灌洗液（BALF）中NO、TNF-α的含量，但其作用与药物剂量有关，，进一步说明寒喘宁口服液具有抗炎、抗过敏作用，是治疗小儿支气管哮喘的有效方剂，值得临床推广。
[Abstract]:Objective: to observe the effects of Hanchuanning oral liquid on asthma symptoms and NO and TNF-a contents in bronchoalveolar lavage fluid (BALF) in asthmatic guinea pigs. To evaluate the therapeutic effect of Hanchuanning oral liquid on inflammatory reaction of asthmatic guinea pigs, and to provide theoretical basis for further clinical application of Hanchuanning oral liquid. Methods: 50 young male guinea pigs were fed adaptively for one week under the same conditions, then were randomly divided into 5 groups: normal control group, asthma model group and dexamethasone control group. Small dose group and large dose group of traditional Chinese medicine. The normal control group was treated with normal saline 1ml instead of ovalbumin intraperitoneal injection, and the remaining four groups were sensitized by intraperitoneal injection of 10% ovalbumin solution 1ml. From the 14th day after injection, the asthmatic model group, dexamethasone tablet control group, small dose Chinese medicine group and large dose Chinese medicine group were placed in 4 liters of fog tank once a day in the afternoon. Ultrasonic atomization of 1% ovalbumin solution for 10 ~ 20 s stimulated asthma, appeared shortness of breath, nod, volt, body tremble and so on, which indicated that the model was successful. The asthmatic guinea pigs were administered simultaneously with dexamethasone tablet control group, low dose Chinese medicine group and high dose Chinese medicine group. The rats in each group were given orally in the interval between the two stimuli on the same day, 3 ml per time, twice a day. The normal control group was treated with normal saline atomization instead of ovalbumin twice a day. Normal control group and asthmatic model group were given 3ml saline twice a day for 2 weeks. The mental activity, food intake, fur color, stool and death of guinea pigs were observed. After 1 hour of last administration, inhaled with 1% ovalbumin solution for 15-20 seconds, the duration of wheezing and other symptoms in guinea pigs was observed. The normal group was replaced by normal saline. In addition to the above drug factors, the rest of the daily feeding methods were the same, and they were free to eat normal guinea pig feed and water. The changes of NO and TNF-a levels in bronchoalveolar lavage fluid (BALF) of guinea pigs were measured to observe the therapeutic effect of Hanchuanning oral liquid. Results: the content of NO,TNF- 伪 in BALF of asthmatic model group was significantly higher than that of normal control group, but the content of NO,TNF- 伪 in BALF was significantly lower than that in asthmatic model group after treated with Hanchuanning oral liquid. Conclusion: Hanchuanning oral liquid can shorten the attack time of asthma and the duration of cold syndrome, reduce the content of NO,TNF- 伪 in bronchoalveolar lavage fluid (BALF), but its effect is related to the dosage of drug, which further indicates that Hanchuanning oral liquid has anti-inflammatory effect. Anti-allergic effect, is an effective prescription for the treatment of bronchial asthma in children, worthy of clinical promotion.
【学位授予单位】：陕西中医学院
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R285.5;R272

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