儿童哮喘与呼吸道菌群相关性的探讨
发布时间：2018-12-12 01:49
【摘要】：[目的]分析支气管哮喘患儿与正常儿童呼吸道菌群的差异。[方法]6-14岁儿童为研究对象,分为三组,每组20人:A、哮喘急性发作期组(A组);B、上述患儿经对症治疗后一周症状缓解为症状缓解期组(B组);C、对照组是正常儿童组(C组)。被检儿童均取咽拭子,提取样本细菌总DNA,进一步对细菌16S rDNA V3可变区进行PCR扩增和DGGE电泳,获得呼吸道中细菌分子指纹图谱,利用Quantity One软件分别对呼吸道菌群进行多样性分析,利用SPSS21.0软件进行统计学分析。[结果]各组呼吸道菌群比较:1、A组呼吸道菌群的条带数S'、多样性指数H'、和优势度指数D'显著高于C组(P0.05)。2、A组与B组呼吸道菌群相比较,细菌的条带数S'、多样性指数H'均有统计学意义,均匀度指数E、优势度指数D'无统计学意义。3、B组与C组相比在条带数S'、多样性指数H'、均匀度指数E和丰富度指数D'均无明显差异。[结论]1、哮喘急性发作期组儿童菌群总体数量更丰富,种类更多,优势菌群更为突出。2、哮喘急性发作期组与症状缓解期组患儿相比,其呼吸道菌群条带数及多样性指数均较高,而均匀度指数及优势度无明显差异。3、哮喘症状缓解期儿童呼吸道菌群与正常儿童相比多样性分析均无统计学差异。
[Abstract]:[objective] to analyze the difference of respiratory tract flora between children with bronchial asthma and normal children. [methods] Children aged 6-14 years were divided into three groups with 20 persons in each group: group A, group A (group A, group); B,), the symptoms were relieved to remission group (group B) after one week of symptomatic treatment; C, the control group was normal children group (group C). The throat swabs were taken from all the children tested, and the total DNA, was extracted from the sample. The 16s rDNA V3 variable region was amplified by PCR and DGGE electrophoresis, and the molecular fingerprint of the bacteria in the respiratory tract was obtained. The diversity of respiratory tract flora was analyzed by Quantity One software and statistical analysis by SPSS21.0 software. [results] Respiratory tract flora of group A was significantly higher than that of group C (P 0.05). 2Respiratory bacterial flora of group A was higher than that of group B (P 0.05), and the number of bands, diversity index (H) and dominance index (D') of group A were significantly higher than those of group C (P 0.05). The number of bands and the diversity index (H') of bacteria were statistically significant, but the evenness index (E) and dominance index (D') were not statistically significant. There was no significant difference in evenness index E and richness index D'. [conclusion] 1. The total number, species and dominant flora of children in acute asthma attack group were more abundant, more and more prominent. 2. Compared with symptom remission group, the children in acute asthma attack group were more abundant than those in symptom remission group. The number of bacterial bands and diversity index of respiratory tract were higher, but there was no significant difference in evenness index and dominance. 3. There was no significant difference in diversity analysis of respiratory tract bacteria between children with asthma symptom remission and normal children.
【学位授予单位】：东南大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R725.6
本文编号：2373658
[Abstract]:[objective] to analyze the difference of respiratory tract flora between children with bronchial asthma and normal children. [methods] Children aged 6-14 years were divided into three groups with 20 persons in each group: group A, group A (group A, group); B,), the symptoms were relieved to remission group (group B) after one week of symptomatic treatment; C, the control group was normal children group (group C). The throat swabs were taken from all the children tested, and the total DNA, was extracted from the sample. The 16s rDNA V3 variable region was amplified by PCR and DGGE electrophoresis, and the molecular fingerprint of the bacteria in the respiratory tract was obtained. The diversity of respiratory tract flora was analyzed by Quantity One software and statistical analysis by SPSS21.0 software. [results] Respiratory tract flora of group A was significantly higher than that of group C (P 0.05). 2Respiratory bacterial flora of group A was higher than that of group B (P 0.05), and the number of bands, diversity index (H) and dominance index (D') of group A were significantly higher than those of group C (P 0.05). The number of bands and the diversity index (H') of bacteria were statistically significant, but the evenness index (E) and dominance index (D') were not statistically significant. There was no significant difference in evenness index E and richness index D'. [conclusion] 1. The total number, species and dominant flora of children in acute asthma attack group were more abundant, more and more prominent. 2. Compared with symptom remission group, the children in acute asthma attack group were more abundant than those in symptom remission group. The number of bacterial bands and diversity index of respiratory tract were higher, but there was no significant difference in evenness index and dominance. 3. There was no significant difference in diversity analysis of respiratory tract bacteria between children with asthma symptom remission and normal children.
【学位授予单位】：东南大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R725.6
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