急性白血病患儿骨髓单个核细胞转铁蛋白受体、铁蛋白、Beclin1、LC3表达及意义

发布时间：2019-01-23 13:33

【摘要】：目的观察急性白血病患儿骨髓单个核细胞(BMMNC)中转铁蛋白受体(TfR)、铁蛋白(FN)及自噬相关基因Beclin1、LC3表达情况,并探讨其意义。方法 46例急性白血病患儿,其中急性淋巴细胞白血病(ALL)患儿30例(ALL组)、急性髓细胞白血病(AML)患儿16例(AML组),另取同时期健康儿童16例(对照组)。取各组骨髓液制备BMMNC,采用逆转录PCR技术检测BMMNC中的TfR、FN、Beclin1、LC3 mRNA,采用Pearson相关分析分析TfR、FN分别与Beclin1、LC3的关系。结果 ALL组BMMNC中TfR、FN、Beclin1、LC3 mRNA的相对表达量较对照组高(P均0.01)。AML组BMMNC中Beclin1、LC3 mRNA的相对表达量较对照组低(P均0.01),TfR、FN mRNA的相对表达量较对照组高(P均0.01)。在ALL组、AML组及对照组,TfR与Beclin1、LC3表达均呈负相关(P均0.05),FN与Beclin1、LC3表达均呈正相关(P均0.05)。结论 TfR、FN在急性白血病患儿BMMNC中表达升高;Beclin1、LC3在ALL中表达增高,而在AML中表达下降;随着自噬活性的增强,TfR表达下调、FN表达上调。
[Abstract]:Objective to investigate the expression of transferrin receptor (TfR), ferritin (FN) and autophagy related gene Beclin1,LC3 in bone marrow mononuclear cells (BMMNC) of children with acute leukemia and its significance. Methods 46 children with acute leukemia, including 30 children with acute lymphoblastic leukemia (ALL) (ALL group), 16 patients with acute myeloid leukemia (AML) (AML group) and 16 healthy children (control group). BMMNC, was prepared from bone marrow fluid of each group. Reverse transcription PCR technique was used to detect TfR,FN,Beclin1,LC3 mRNA, in BMMNC. Pearson correlation analysis was used to analyze the relationship between TfR,FN and Beclin1,LC3. Results the relative expression of TfR,FN,Beclin1,LC3 mRNA in BMMNC in ALL group was higher than that in control group (P 0.01). The relative expression of Beclin1,LC3 mRNA in BMMNC in). AML group was lower than that in control group (all P 0.01), TfR,). The relative expression of FN mRNA was higher than that of control group (P 0.01). In ALL group, AML group and control group, there was a negative correlation between TfR and Beclin1,LC3 expression (P0. 05), FN and Beclin1,LC3 expression were positive correlation (P0. 05). Conclusion the expression of TfR,FN increased in BMMNC of children with acute leukemia, increased in ALL, decreased in AML, down-regulated in TfR and up-regulated in FN with the increase of autophagy activity.
【作者单位】：郑州大学第一附属医院;
【分类号】：R733.71

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