不同肠道病毒感染手足口病患儿儿茶酚胺、S-100蛋白及D-乳酸水平变化及其临床意义

发布时间：2019-04-26 18:42

【摘要】：目的探讨不同肠道病毒感染手足口病(HFMD)患儿儿茶酚胺(CA)、S-100蛋白和D-乳酸水平的变化及其临床意义。方法选取2014年4—7月在昆明市儿童医院感染科住院并确诊为HFMD的患儿129例。使用GC-2016γ放射免疫计数仪采用放射免疫法进行CA测定[主要包括去甲肾上腺素(NE)、肾上腺素(AD)、多巴胺(DA)];使用美国宝莱特ELx800NB酶标仪采用酶联免疫法进行S-100蛋白测定;使用美国宝莱特ELx800NB酶标仪采用酶联免疫法进行D-乳酸测定;使用德国ABI公司生产的7500PCR扩增仪采用实时荧光定量PCR方法进行粪便病原体的检测及分型。结果 129例HFMD患儿中,肠道病毒71型(EV71)阳性57例(44.2%),柯萨奇病毒A组16型(Cox A16)阳性26例(20.2%),其他肠道病毒阳性20例(15.5%),肠道病毒阴性26例(20.1%)。不同肠道病毒感染HFMD患儿NE、AD、DA、S-100蛋白水平比较,差异无统计学意义(P0.05);不同肠道病毒感染HFMD患儿D-乳酸水平比较,差异有统计学意义(P0.05),其中EV71阳性HFMD患儿D-乳酸水平高于其他肠道病毒阳性、肠道病毒阴性患儿。结论不同肠道病毒感染HFMD患儿CA、S-100蛋白水平间无差异,而EV71感染HFMD患儿D-乳酸水平较高,存在肠屏障通透性增加。
[Abstract]:Objective to investigate the changes and clinical significance of catecholamine (CA),-100 protein and D-lactic acid in patients with hand-foot-mouth disease (HFMD) infected with different enterovirus. Methods from April to July, 2014, 129 children who were hospitalized in Kunming Children's Hospital Infectious Department and diagnosed as HFMD were selected. CA was measured by radioimmunoassay with GC-2016 纬 radioimmunoassay [mainly including norepinephrine, (NE), epinephrine, (AD), dopamine (DA)]. Sal 100 protein was determined by enzyme linked immunosorbent assay (Elisa), D lactic acid (D lactic acid) was determined by enzyme linked immunosorbent assay (Elisa), and D lactic acid (D lactic acid) was determined by enzyme linked immunosorbent assay (Elisa) with ELx800NB. Real-time fluorescence quantitative PCR was used to detect and type faecal pathogens using 7500PCR amplification instrument produced by ABI company in Germany. Results among the 129 children with HFMD, 57 (44.2%) were positive for enterovirus 71 (EV71), 26 (20.2%) for coxsackievirus A (Cox A16) and 20 (15.5%) for other enteroviruses. 26 cases (20.1%) were negative for enterovirus. There was no significant difference in the level of NE,AD,DA,S-100 protein between different enterovirus infected children with HFMD (P0.05). The level of D-lactic acid in HFMD patients with different enterovirus infection was significantly higher than that in other enterovirus positive children with EV71-positive HFMD (P0.05). Conclusion there is no difference in the level of CA,S-100 protein among children with HFMD infected with different enterovirus, but the level of D-lactic acid in HFMD infected with EV71 is higher, and the permeability of intestinal barrier is increased.
【作者单位】：昆明医科大学附属儿童医院
【基金】：云南省昆明市卫生人才培养项目及十百千工程项目(2016-SW省-53) 云南省昆明市科技计划重点项目(2015-02-S-01226)
【分类号】：R725.1

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