冷自体血心脏停搏液对未成熟心肌HSP70、SOD的影响

发布时间：2019-05-06 19:14

【摘要】：目的：对比研究冷自体血心脏停搏液与HTK液在婴幼儿体外循环室间隔缺损修补术心肌缺血与再灌注早期心肌HSP70、SOD释放的影响,揭示冷自体血心脏停搏液在体外循环心脏直视手术中对未成熟心肌的内源性保护作用。方法：年龄≤1岁行体外循环室间隔缺损修补术的患儿40例,随机分为实验组与对照组各20例。主动脉阻断后,分别用冷自体血心脏停搏液、HTK液经主动脉根部进行灌注。于主动脉阻断前即刻、主动脉开放后第1分钟及第15分钟抽取冠状静脉窦回流血及体静脉回流血,应用酶联免疫吸附法(ELISA法)检测HSP70、SOD浓度。运用t检验进行统计学处理。结果：同组主动脉开放后与主动脉阻断前比较,HSP70、SOD浓度明显升高(P0.05,P0.01)。两组比较,主动脉阻断前两组之间差异无显著性(P0.05),主动脉开放后冷自体血心脏停搏液组HSP70、SOD浓度明显高于晶体心脏停搏液组(P0.05)。结论：婴幼儿体外循环室间隔缺损修补术心肌缺血与再灌注早期心肌HSP70、SOD释放增加。冷自体血心脏停搏液在婴幼儿体外循环心肌缺血与再灌注早期的过程中可促进HSP70、SOD释放,对婴幼儿心肌有内源性保护作用。
[Abstract]:Objective: to compare the effects of cold autologous blood cardioplegia solution and HTK solution on myocardial HSP70,SOD release during myocardial ischemia and reperfusion in infants with ventricular septal defect repair by cardiopulmonary bypass (CPB). This study revealed the endogenous protective effect of cold autologous cardioplegic solution on immature myocardium during open heart surgery with cardiopulmonary bypass (CPB). Methods: forty children with ventricular septal defect repair by cardiopulmonary bypass were randomly divided into two groups: experimental group (n = 20) and control group (n = 20). After aortic cross-clamping, cold autologous blood cardioplegic solution and HTK solution were perfused through aortic root respectively. HSP70,SOD concentration was measured by enzyme-linked immunosorbent assay (ELISA) immediately before aortic cross-clamping and 1 and 15 minutes after aortic opening. The coronary sinus and systemic venous circulation were extracted from the coronary sinus and systemic venous circulation immediately before aortic cross-clamping and 1 and 15 minutes after aortic opening. T-test was used for statistical analysis. Results: the concentration of HSP70,SOD in the same group was significantly higher than that before aortic cross-clamping (P 0.05, P0.01). There was no significant difference between the two groups before aortic occlusion (P0.05). The concentration of HSP70,SOD in cold autogenous blood cardioplegia group after aortic opening was significantly higher than that in lens cardioplegia group (P0.05). Conclusion: myocardial HSP70,SOD release increased in the early stage of myocardial ischemia and reperfusion in infants with ventricular septal defect repair by cardiopulmonary bypass. Cold autogenous cardioplegia can promote the release of HSP70,SOD during the early stage of myocardial ischemia and reperfusion in infants with cardiopulmonary bypass, and has endogenous protective effect on infant myocardium.
【学位授予单位】：遵义医学院
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R726.5

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