呼吸道合胞病毒毛细支气管炎患儿外周血T细胞亚群、IL-4、IFN-γ、IL-17的水平及意义

发布时间：2019-05-07 07:35

【摘要】：目的察探究外周血中Treg细胞、T细胞群炎性细胞因子IL-4 IFN-γIL-17在RSV感染的毛细支气管炎患儿发病病程展中的意义方法选2015年7~2016年6期间临沂人民院收治的毛细支气管炎院患儿1038例作临床研究对象,使用免疫荧法对患儿咽部分泌物行7种道病毒检选RSV检性的患儿88例,按照疾病展程度将患儿轻症组45例和伴明显喘息症状的症组43例选时期因非感染疾病入院的患儿作对照组45例用流式细胞仪检患儿外周血中Treg细胞T细胞群情况用酶联免疫试验ELISA抗夹心法检患儿血清中IL-4 IFN-γIL-17等炎性细胞因子表达水平结果1 1038例毛细支气管炎患儿中7种道病毒总检率是42.29%,中RSV 65.83%2症组和轻症组RSV感染的毛细支气管炎患儿外周血Treg细胞均明显于常对照组儿童P0.05症组RSV感染的毛细支气管炎患儿外周血Treg细胞于轻症组患儿P0.05,组间差明显统计学意义3症组和轻症组RSV感染的毛细支气管炎患儿常对照组儿童较,外周血中CD8~+T细胞均于常对照组儿童P0.05,CD4~+T细胞和CD4~+/CD8~+T细胞值均高于常对照组儿童P0.05症组患儿外周血CD8~+T细胞于轻症组患儿P0.05,CD4~+T细胞和CD4~+/CD8~+T细胞值高于轻症组患儿P0.05,差统计学意义4症组和轻症组RSV感染的毛细支气管炎患儿外周血IL-4 IL-17水平均明显高于常对照组儿童P0.05,IFN-γ水平明显于常对照组儿童P0.05症组患儿外周血IL-4 IL-17水平高于轻症组患儿P0.05,IFN-γ水平于轻症组患儿P0.05,组间差统计学意义。结论1患儿外周血Treg细胞RSV感染的毛细支气管炎发展呈负相关2患儿外周血CD8~+T细胞RSV感染的毛细支气管炎发展呈负相关,外周血CD4~+T细胞和CD4~+/CD8~+T细胞值RSV感染发展呈相关3患儿外周血中IL-4 IL-17水平RSV感染展程度呈相关,IFN-γ水平疾病展呈负相关。
[Abstract]:Objective to investigate the Treg cells in peripheral blood. Significance of T-cell inflammatory cytokine IL-4 IFN- 纬-IL-17 in the pathogenesis of RSV-infected bronchiolitis methods 1038 children with bronchiolitis admitted to Linyi people's Hospital from July 2015 to June 2016 were selected as clinical subjects. Using immunofluorescence method, 88 children with RSV were tested for 7 kinds of viruses in pharyngeal secretion. According to the degree of disease spread, 45 cases of mild group and 43 cases of group with obvious wheezing symptoms were selected as control group, 45 cases of non-infectious diseases were selected as control group. Flow cytometry was used to detect the T-cell population of Treg cells in peripheral blood of children with mild disease and 43 cases with obvious wheezing symptoms. Enzyme-linked immunosorbent assay (ELISA) was used to detect the expression of IL-4 IFN- 纬-IL-17 and other inflammatory cytokines in serum of children with bronchiolitis. The total detection rate of 7 kinds of viruses in 1 1038 children with bronchiolitis was 42.29%. Treg cells in peripheral blood of patients with bronchiolitis infected with RSV in RSV 65.83% 2 group and mild group were significantly higher than those in children with bronchiolitis infected with RSV in the normal control group (P0.05) and in the mild group (P0.05). The difference between the three groups was statistically significant. The CD8~ T cells in the peripheral blood of children with bronchiolitis infected by RSV in the three groups were significantly higher than those in the control group (P0.05, P 0.05, P < 0.05). The values of CD4~ T cells and CD4~ / CD8~ T cells were higher than those of the normal control group in the peripheral blood CD8~ T cells, CD4 ~ T cells and CD4~ / CD8~ T cells in the mild group were higher than those in the mild group (P 0.05, P < 0.05, P < 0.01, P < 0.01). The IL-4 IL-17 level in peripheral blood of bronchiolitis patients with RSV infection was significantly higher than that in normal controls (P 0.05, P < 0.01), but there was no significant difference between the two groups (P < 0.05). The level of IFN- 纬 in peripheral blood was significantly higher in P05 group than that in mild group (P0.05), and the level of IFN-纬 in mild group was significantly higher than that in mild group (P0.05), and the difference between the two groups was statistically significant. Conclusion (1) the development of bronchiolitis infected with RSV of peripheral blood Treg cells is negatively correlated with the development of bronchiolitis infected with RSV of peripheral blood CD8~ T cells. The peripheral blood CD4~ T cells and CD4~ / CD8~ T cell values were correlated with the development of RSV infection. 3. The degree of RSV infection in peripheral blood was correlated with the degree of RSV infection in the peripheral blood, and there was a negative correlation between the disease spread of IFN- 纬 level and the peripheral blood IL-4 IL-17 T cells.
【学位授予单位】：青岛大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R725.6

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