腹腔注射氢气与富氢生理盐水对新生大鼠高氧肺损伤的作用研究

发布时间：2019-06-13 16:05

【摘要】：目的对比分析腹腔注射氢气(H2)和富氢生理盐水对高氧致新生大鼠肺损伤的作用。方法将SD新生大鼠分为6组(每组10只),A组(空气组)、B组(空气+富氢生理盐水组)、C组(空气+H2组)、D组(高氧组)、E组(高氧+富氢生理盐水组)、F组(高氧+H2组)。A、B、C组置于空气中,D、E、F组置于95%O2中,B、E组每日腹腔注射富氢生理盐水2次(10 mL/kg),C、F组每日腹腔注射H22次(10mL/kg),A、D组每日腹腔注射普通生理盐水2次(10mL/kg)。于实验第15天收集肺组织和血清标本,苏木精-伊红(HE)染色后光镜下观察肺组织病理改变和辐射状肺泡计数(RAC),碱水解法检测肺组织羟脯氨酸(HYP)、血清丙二醛(MDA)、超氧化物歧化酶(SOD)水平,免疫组织化学SP法检测肌成纤维细胞表型标志α-平滑肌肌动蛋白(α-SMA)表达。结果与A组相比,D组RAC、血清SOD活力明显降低,HYP、MDA水平及α-SMA表达均明显增加,H2干预可显著缓解高氧所致上述变化,腹腔注射H2较腹腔注射富氢生理盐水效果更佳。结论 H2可一定程度减轻高氧导致的肺氧化损伤、发育受阻及纤维化指标,腹腔注射H2较腹腔注射高氢生理盐水效果更佳。
[Abstract]:Objective to compare the effects of intraabdominal injection of hydrogen (H2) and hydrogen-rich saline on lung injury induced by hyperoxia in neonatal rats. Methods SD neonatal rats were divided into 6 groups (10 rats in each group: air group), B group (air hydrogen enriched saline group), C group (air H2 group), D group (hyperoxia group), E group (hyperoxia H2 group). A, B, C group in air, D, E, F group in 95%O2, B. Group E was intraperitoneally injected with hydrogen-enriched saline twice a day (10mL/kg), C, group F was injected with H22 times a day (10mL/kg), group A and group D were injected with normal saline twice a day (10mL/kg). On the 15th day of the experiment, lung tissue and serum samples were collected. The pathological changes of lung tissue and radiative alveolar count (RAC), alkaline hydrolysis were observed under light microscope to detect the level of malondialdehyde (MDA) (SOD) in serum of hydroxyproline (HYP),. The expression of 伪-smooth muscle actin (伪-SMA), a phenotypic marker of myofibroblasts, was detected by immunohistochemical SP. Results compared with group A, the activity of SOD in serum of group D was significantly decreased, the level of HYP,MDA and the expression of 伪-SMA were significantly increased. H2 intervention could significantly alleviate the above changes induced by hyperoxia, and the effect of intraperitoneal injection of H2 was better than that of intraabdominal injection of hydrogen-enriched saline. Conclusion H _ 2 can reduce the oxidative injury of lung caused by hyperoxia to a certain extent, and the indexes of development obstruction and fibrosis can be alleviated to a certain extent. The effect of intraabdominal injection of H _ 2 is better than that of hyperhydrogen saline.
【作者单位】：重庆医科大学附属儿童医院PICU;重庆医科大学附属儿童医院呼吸二病房/儿童发育疾病研究教育部重点实验室;南京大学医学院金陵医院烧伤整形科;第二军医大学海军医学系潜水医学部;
【基金】：重庆市科委基金资助项目(cstc2013jcyjA10031)
【分类号】：R722

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