SPIO-shRNA分子探针注射不同时间点荷瘤裸鼠肿瘤区域组织信号变化及MRI最佳扫描时间

发布时间：2018-01-10 16:30

  本文关键词：SPIO-shRNA分子探针注射不同时间点荷瘤裸鼠肿瘤区域组织信号变化及MRI最佳扫描时间　出处：《吉林大学学报(医学版)》2016年06期 　论文类型：期刊论文

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【摘要】：目的:探讨注射SPIO-shRNA分子探针不同时间点的荷瘤裸鼠肿瘤区域组织信号强度变化情况,阐明分子探针活体磁共振成像(MRI)机制和最佳扫描时间。方法:探针注射剂量为18 mg·kg~(-1);将30只BALB/c荷瘤裸鼠随机分为6组,每组5只,分别于探针注射前和注射后12、24、27、30和36h等6个时间点行MRI检查,并测量裸鼠肿瘤组织及对侧肌肉组织在各时间点的信号强度变化;每次检查完成后立即处死裸鼠,取出肿瘤组织及肌肉组织行HE及普鲁士蓝染色,并在光学显微镜下观察裸鼠肿瘤组织的形态表现。结果:与注射前比较,探针注射后12、24和27h裸鼠肿瘤组织T1WI信号强度均升高(P0.05),12、24、27、30和36h肿瘤组织T2WI和T2*GRE信号强度均降低(P0.05或P0.01);探针注射后27h肿瘤组织T1WI信号强度最高;探针注射后27h肿瘤组织T2WI和T2*GRE信号强度低于注射后12、24、30和36h(P0.01)。与注射前比较,注射后24h时裸鼠对侧肌肉组织信号强度降低(P0.05)。HE染色,裸鼠肿瘤组织结构紊乱,细胞核异型性较多;普鲁士蓝染色,在注射后各时间点上均存在蓝染的Fe颗粒,注射后27h时蓝染Fe颗粒最多。结论:SPIO-shRNA分子探针能成功靶向裸鼠肿瘤组织,且MRI最佳的扫描时间为探针注射后27h。
[Abstract]:Objective: to investigate the changes of signal intensity in tumor region of nude mice injected with SPIO-shRNA molecular probe at different time points. To elucidate the mechanism and optimal scanning time of molecular probe in vivo magnetic resonance imaging (MRI). Methods: the injection dose of the probe was 18 mg 路kg ~ (-1). Thirty BALB/c bearing nude mice were randomly divided into 6 groups, 5 in each group, and MRI was performed at 6 time points before and after injection. The signal intensity of tumor tissue and contralateral muscle tissue in nude mice at different time points were measured. The nude mice were killed immediately after each examination, and the tumor tissues and muscle tissues were taken out for HE and Prussian blue staining, and the morphological features of tumor tissues were observed under optical microscope. Results: compared with before injection. The signal intensities of T1WI in tumor tissues of nude mice were increased at 12h and 27h after the injection of the probe. The signal intensity of T2WI and T2GRE in tumor tissues decreased at 30 and 36 hours (P 0.05 or P 0.01). The signal intensity of T1WI was the highest in the tumor tissue 27 hours after the injection of the probe. The signal intensity of T2WI and T2GRE in tumor tissue at 27 h after injection was lower than that in 1240 h and 36 h after injection, which was compared with that before injection. 24 hours after injection, the signal intensity of contralateral muscle tissue decreased in nude mice. Prussian blue staining showed the presence of blue-stained Fe particles at all time points after injection. Conclusion the MRI molecular probe can successfully target tumor tissue of nude mice, and the best scanning time of MRI is 27h after injection.
【作者单位】： 重庆医科大学附属第一医院放射科;
【基金】：国家自然科学基金资助课题(81171366) 卫计委国家临床重点专科建设项目资助课题[(2013)544]
【分类号】：R445.2;R737.31
【正文快照】： 卵巢癌是女性生殖系统最常见的恶性肿瘤之一,早期发现并进行干预者生存率可达90%。但由于其早期临床症状不典型,且肿瘤位居盆腔深处,多数患者发现时已属晚期。影像学诊断在卵巢癌发现和预后评估中具有重要的价值,目前临床常用的方法主要为电子计算机断层扫描(CT)、磁共振成像(，

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