MR成像活体示踪SPIO-shRNA分子探针的生物分布

发布时间：2018-01-11 14:28

本文关键词：MR成像活体示踪SPIO-shRNA分子探针的生物分布　出处：《四川大学学报(医学版)》2016年04期 　论文类型：期刊论文

【摘要】：目的探讨磁共振成像(MR成像)能否示踪超顺磁性氧化铁(superparamagnetic iron oxide,SPIO)-shRNA分子探针在活体动物主要脏器的生物分布。方法 6只新西兰大白兔,注射SPIO-shRNA分子探针(含铁量为9.6mg/kg),原子吸收光谱法(atomic absorption spectrometry,AAS)测量注射前30 min及注射后1 min、3min、5min、10min、15min、30min、1h、2h、3h、6h、12h、24h的血浆铁质量浓度以分析其药代动力学。6只昆明(KM)小鼠于尾静脉注射含铁量为4.8mg/kg的SPIO-shRNA分子探针,MR成像活体示踪探针在小鼠体内主要脏器的生物分布。96只KM小鼠于未给药及给药后1d、3d、5d、7d、9d、11d、14d,取肝、脾、肾、脑及肌肉组织,AAS测定各脏器组织探针铁含量,同时行普鲁士蓝染色并与MR结果对照分析。结果本探针的药代动力学符合二室模型,半衰期为(3.692±0.196)h。MR显示探针在正常小鼠体内主要分布于肝脏与脾脏,代谢缓慢,于2周左右完全从肝、脾代谢清除;AAS对脏器探针铁含量的测定与普鲁士蓝染色证实了MR成像结果。结论本研究明确了MR成像可用于活体示踪SPIO-shRNA分子探针在动物体内主要脏器的生物分布,为进一步利用MR无创监测探针在动物体内的治疗作用提供了依据。
[Abstract]:Objective to investigate whether magnetic resonance imaging (Mr) can trace superparamagnetic iron oxide of superparamagnetic ferric oxide. The biological distribution of SPIO)-shRNA molecular probe in the main organs of living animals. Methods six New Zealand white rabbits were used. Atomic absorption spectrometry (atomic absorption spectrometry) was injected with SPIO-shRNA molecular probe (iron content was 9.6 mg / kg ~ (-1)). AAS was used to measure 30 min before injection and 1 min 3 min after injection, 5 min 10 min 10 min 15 min 30 min 30 min 1 hour 2 h ~ 3 h ~ 3 h ~ 6 h ~ 6 h ~ (12) h. 24 h plasma iron concentration was used to analyze the pharmacokinetics of 6 Kunming KM mice. A SPIO-shRNA molecular probe with an iron content of 4.8 mg / kg was injected into the tail vein of 6 Kunming KM mice. The biological distribution of Mr imaging tracer probe in the main organs of mice. 96 km mice were taken liver, spleen and kidney at 1 day, 3 days, 5 days, 7 days, 7 days, 9 days and 14 days, respectively. The content of probe iron in brain and muscle tissue was determined by AAS. Prussian blue staining was performed at the same time and the results were compared with Mr results. Results the pharmacokinetics of the probe was in accordance with the two-compartment model. The half-life was 3.692 卤0.196 h.MR showed that the probe was mainly distributed in the liver and spleen of normal mice, and the metabolism was slow and completely removed from the liver and spleen at about 2 weeks. The results of Mr imaging were confirmed by AAS and Prussian blue staining. Conclusion it is clear that Mr imaging can be used to trace SPIO-shRNA molecular probe in vivo in animals. The biological distribution of the organs. It provides a basis for the further use of Mr noninvasive monitoring probe in the treatment of animal in vivo.
【作者单位】：重庆医科大学附属第一医院放射科;
【基金】：国家自然科学基金(No.81171366) 国家临床重点专科建设项目[国卫办医函(2013)544]资助
【分类号】：R445.2
【正文快照】：

【参考文献】