C6胶质瘤肝脏转移模型的建立及其磁共振检测

发布时间：2018-03-04 06:14

本文选题：胶质瘤　切入点：动物模型　出处：《重庆医科大学学报》2015年09期 　论文类型：期刊论文

【摘要】：目的:建立裸鼠胶质瘤肝脏转移模型,探讨3.0T磁共振成像(magnetic resonance imaging,MRI)对模型肝脏转移灶检测的可行性。方法:手术暴露裸鼠肝门静脉,经肝门静脉注射C6胶质瘤细胞至36只裸鼠肝脏,分别于注射胶质瘤后的7 d、14 d和21 d随机抽取建模裸鼠12只行3.0T MRI扫描,之后取建模裸鼠肝脏组织对其进行大体形态学观察、HE染色,比较3种方法对肿瘤转移灶的检出能力以及所测肿瘤结节大小的差异,并使用S100beta进行免疫组学染色。结果:MRI影像学对肿瘤转移灶的检出时间要晚于HE染色(Waldχ2=5.01,P=0.025),而较大体标本改变出现时间无差异(Waldχ2=0.03,P=0.853);且14 d与21 d时MRI所测肿瘤结节直径与HE染色以及大体标本所见肿瘤结节直径之间亦有差异(F14 d=14.28,P=0.000;F21 d=1 123.23,P=0.000)。组织切片S100beta免疫组化结果证实肝脏肿瘤结节为C6胶质瘤。且肿瘤转移灶在MRI T2WI上呈高信号改变,对较大肿瘤结节3.0T MRI能显示其内细节及瘤周水肿。结论:该方法可成功构建胶质瘤血行肝脏转移模型;且3.0T MRI可用于该模型鼠肿瘤转移灶的检测。
[Abstract]:Objective: to establish a model of hepatic metastasis in nude mice, and to investigate the feasibility of detecting hepatic metastases by magnetic resonance imaging (resonance) of 3.0T. Methods: the hepatic portal vein of nude mice was exposed during operation. C6 glioma cells were injected into the liver of 36 nude mice by portal vein injection. 12 nude mice were randomly selected for 3.0T MRI scan on day 14 and day 21 after injection of glioma. Then the liver tissue of nude mice was observed by HE staining, and the ability of detecting metastatic tumor and the difference of tumor nodule size between the three methods were compared. S100 beta was used for immunostaining. Results the detection time of tumor metastases was later than that of Wald 蠂 ~ 2 ~ 2 ~ 2 ~ 2 ~ 2 ~ 2 ~ 2 ~ 2 ~ 1 ~ + -HE staining, but there was no difference in the time of appearance of the larger body samples, and the diameter and diameter of tumor nodules measured by MRI at 14 d and 21 d were not different. There was also a difference between HE staining and the diameter of tumor nodules in gross specimens. The liver tumor nodules were confirmed to be C6 gliomas by immunohistochemical staining of S100 beta on MRI T _ 2WI, and the tumor metastases showed hyperintense changes on MRI T _ 2WI. Conclusion: this method can successfully construct the glioma model of liver metastasis, and 3.0T MRI can be used to detect the tumor metastasis in the model rats.
【作者单位】：重庆医科大学附属儿童医院儿科研究所儿童发育疾病研究教育部重点实验室;重庆医科大学附属儿童医院放射科;
【基金】：国家自然科学基金资助项目(编号:81201744)
【分类号】：R445.2;R739.4

【参考文献】