VEGFR-2分子探针超声分子成像评价小鼠下肢缺血性血管新生的可行性

发布时间：2018-03-23 15:52

本文选题：血管内皮生长因子受体-　切入点：超声　出处：《中国老年学杂志》2017年17期

【摘要】：目的探讨血管内皮细生长因子受体(VEGFR)-2分子探针的超声分子成像评价小鼠下肢缺血性血管新生可行性。方法将10只实验小鼠麻醉后结扎一侧下肢股动脉制备下肢缺血模型,术后第7天,所有小鼠随机经尾静脉注入携抗小鼠VEGFR-2靶向微泡(Mb VEGFR-2)和携同型抗体对照微泡(Mbc),并行超声分子检查,测量双下肢的显影强度(VI)值,并处死小鼠后对骨骼肌进行免疫组化检查。结果经过8 min循环时间后,Mb VEGFR-2组小鼠可见明显的超声显影,Mbc组小鼠可见轻度超声显影,但其显影强度明显弱于Mb VEGFR-2组;在非缺血小鼠下肢,经过8 min循环时间后,Mb VEGFR-2组和Mbc组小鼠均未见明显的超声显影;缺血下肢实验小鼠Mb VEGFR-2的VI值明显高于Mbc和非缺血下肢实验小鼠(P0.01),缺血下肢实验小鼠Mbc的VI值高于非缺血下肢实验小鼠(P0.05),非缺血下肢骨骼肌VI值在Mb VEGFR-2和Mbc之间差异无统计学意义(P0.05);DAB染色结果显示下肢缺血小鼠骨骼肌血管有VEGFR-2表达,而下肢非缺血小鼠骨骼肌血管中未见VEGFR-2表达。结论采用携VEGFR-2分子探针的超声成像对缺血下肢新血管进行造影可行,为评价缺血性血管新生提供了病理学依据。
[Abstract]:Objective to evaluate the feasibility of ultrasound molecular imaging with vascular endothelial fine growth factor receptor (VEGF) receptor VEGFR-2 molecular probe to evaluate ischemic angiogenesis in mice lower extremity. Methods Ten experimental mice were anesthetized and ligated one side of lower extremity femoral artery to make lower extremity ischemia model. On the 7th day after operation, all mice were randomly injected with anti-mouse VEGFR-2 targeting microbubbles Mb VEGFR-2) and Ctrip antibody control group MbFR-2. The development intensity of both lower extremities was measured by ultrasonic molecular examination. The skeletal muscle was examined by immunohistochemistry after the mice were killed. Results after 8 min of circulating time, the mice in Mb VEGFR-2 group could be seen mild ultrasound development in MBC group, but the development intensity was significantly lower than that in Mb VEGFR-2 group. In the lower extremities of non-ischemic mice, there was no obvious ultrasound imaging in Mb VEGFR-2 group and Mbc group after 8 min cycle time. The VI value of Mb VEGFR-2 in ischemic lower extremity mice was significantly higher than that in Mbc and non-ischemic lower extremity mice, the VI value of Mbc in ischemic lower limb mice was higher than that in non-ischemic lower extremity experimental mice, and the VI value of non-ischemic lower limb skeletal muscle was between Mb VEGFR-2 and Mbc. The results of DAB staining showed that there was VEGFR-2 expression in skeletal muscle of mice with lower extremity ischemia. The expression of VEGFR-2 was not found in the skeletal muscle vessels of non-ischemic mice. Conclusion Ultrasonography with VEGFR-2 molecular probe is feasible for evaluating ischemic vascularization of lower extremity, which provides a pathological basis for the evaluation of ischemic angiogenesis.
【作者单位】：华中科技大学同济医学院附属武汉儿童医院(武汉市妇幼保健院);
【分类号】：R445.1

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