裸鼠乳腺癌淋巴转移模型的CEUS表现

发布时间：2018-04-02 08:24

本文选题：超声造影　切入点：乳腺肿瘤　出处：《中国介入影像与治疗学》2015年08期

【摘要】：目的通过CEUS观察裸鼠MDA-MB-231乳腺癌原位肿瘤及腋窝淋巴结的增强情况。方法雌性5周龄BALB/C裸鼠8只,于2只裸鼠右侧第二对乳腺脂肪垫下分别注射0.1ml MDA-MB-231乳腺癌细胞悬液,成瘤2周后,解剖取得肿瘤块后原位种植于另外6只裸鼠乳垫下,建立裸鼠乳腺癌淋巴转移模型。通过大体和常规超声观察原位肿瘤和同侧腋窝肿大淋巴结情况,在接种后第6周,采用经鼠尾静脉和经肿瘤周围皮下分别注射0.1ml SonoVue超声造影剂进行CEUS检查,造影结束后解剖裸鼠获得原始肿瘤及肿大淋巴结进行病理检查。结果 1接种后第6天,6只裸鼠接种部位均长出肿瘤,成瘤率100%(6/6),常规超声观察原始肿瘤内血流较丰富,后期出现液化坏死灶和微小钙化灶,大体和常规超声观察均未发现明显腋窝肿大淋巴结;2经鼠尾静脉CEUS示所有裸鼠的原始肿瘤均为不均匀增强,肿瘤内出现范围不等的充盈缺损区,病理诊断原始肿瘤块为浸润性癌;3经肿瘤周围皮下CEUS检出1枚腋窝淋巴结(病理结果为转移性淋巴结),表现为不均匀增强,裸鼠解剖后共检出3只裸鼠各有1枚腋窝转移性淋巴结,以病理结果为金标准,CEUS对乳腺癌转移淋巴结的检出率为33.33%(1/3)。结论 CEUS在裸鼠乳腺癌淋巴转移模型中具有一定的应用价值。
[Abstract]:Objective to observe the enhancement of in situ tumor and axillary lymph node of MDA-MB-231 breast cancer in nude mice by CEUS. Methods 8 female 5 week old BALB/C nude mice were injected with 0.1ml MDA-MB-231 breast cancer cell suspension under the second right breast fat pad in 2 nude mice. After 2 weeks of tumorigenesis, tumor masses were obtained and implanted in situ under the breast mats of other 6 nude mice to establish lymphatic metastasis model of breast cancer in nude mice. The tumor in situ and enlarged lymph nodes in axillary armpit were observed by gross and routine ultrasound. At the 6th week after inoculation, 0.1ml SonoVue ultrasound contrast agent was injected into the tail vein of the rat and subcutaneously around the tumor respectively for CEUS examination. Results 1 on the 6th day after inoculation, all the 6 nude mice grew tumors, and the tumorigenesis rate was 100% / 6%. The blood flow in the original tumor was abundant by conventional ultrasound. There were liquefaction necrosis foci and small calcification foci in the later stage. No obvious axillary enlarged lymph nodes were found in gross or conventional ultrasound. CEUS showed that the original tumors of all nude mice were heterogeneous enhancement. An axillary lymph node (axillary lymph node) was detected by subcutaneous CEUS in the tumor with different filling defect areas, and the primary tumor was diagnosed by pathology as invasive carcinoma. One axillary metastatic lymph node was detected in each of 3 nude mice after dissection. The positive rate of CEUS in metastatic lymph nodes of breast cancer was 33.33 / 1 / 3 according to the pathological results. Conclusion CEUS is valuable in lymphatic metastasis of breast cancer in nude mice.
【作者单位】：宁夏医科大学临床医学院;宁夏医科大学总医院超声科;
【基金】：国家自然科学基金(81360225) 2013年度宁夏自然科学基金项目(NZ13156)
【分类号】：R-332;R445.1;R737.9

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