磁共振示踪小鼠树突状细胞归巢淋巴结的初步研究

发布时间：2018-04-02 15:11

本文选题：超顺磁性氧化铁纳米颗粒　切入点：磁共振成像　出处：《重庆医科大学学报》2015年09期

【摘要】：目的:尝试采用磁共振成像(magnetic resonance imaging,MRI)监测磁标记树突状细胞(dendritic cell,DC)归巢引流淋巴结。方法:DC由小鼠骨髓细胞经诱导分化获得。探针为烷基化低分子量聚乙烯亚胺(PEI2k)包裹的超顺磁性氧化铁(superparamagnetic iron oxide,SPIO)纳米颗粒,标记方法为成熟DC与探针共孵育过夜。收集细胞,行标记效果、细胞分子表型分析。将标记的成熟DC注入小鼠足底,行不同时间点MRI,观察DC归巢淋巴结,72 h后,取乆窝淋巴结送病理检查。结果:DC纯度高达90%。普鲁士兰染色和激光共聚焦成像显示探针位于细胞内。流式细胞术(fluorescence activated cell sorting,FACS)分析表明,标记过程对DC的成熟表型无明显影响。体内MRI和淋巴结病理显示,24 h后标记DC在淋巴结内聚集。结论:采用N-alkylPEI2k/SPIO纳米探针标记DC后,MRI可以清楚地监测其在体内归巢淋巴结的情况。
[Abstract]:Objective: to monitor the homing and draining lymph nodes of dendritic cells (DC) by magnetic resonance imaging (MRI).Methods the mouse bone marrow cells were induced to differentiate into 1% DC.The probe consisted of superparamagnetic iron oxide nanoparticles coated with alkylated low molecular weight polyethylene imide (PEI2k). The labeling method was mature DC and probe incubated overnight.The cells were collected for labeling and molecular phenotypic analysis.The labeled mature DC was injected into the plantar of mice, and MRI was performed at different time points. After 72 hours of DC homing lymph nodes were observed, the lymph nodes were collected for pathological examination.As a result, the purity of 10% DC was as high as 90%.Prussian blue staining and laser confocal imaging showed that the probe was in the cell.Fluorescence activated cell sorting analysis showed that the labeling process had no significant effect on the mature phenotype of DC.In vivo MRI and lymph node pathology showed that DC were clustered in lymph nodes 24 h later.Conclusion: N-alkylPEI2k/SPIO labeled DC can clearly monitor the homing lymph nodes in vivo.
【作者单位】：重庆医科大学附属儿童医院检验科;四川大学国家生物医学材料研究中心;重庆医科大学附属儿童医院放射科;
【分类号】：R445.2

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