超声造影对土三七诱导的大鼠肝窦阻塞综合征的诊断价值

发布时间：2018-05-01 11:03

本文选题：超声造影 + 土三七　；参考：《中南大学》2014年硕士论文

【摘要】：第一部分土三七诱导大鼠HSOS模型的建立与评价目的：采用PBS、土三七水煎液大鼠灌胃,模拟临床患者服药情况,建立稳定、可复制的且能有效模拟临床发病过程的大鼠HSOS模型,并给予综合评价。方法：30只健康雄性SD大鼠,随机分为两组：PBS正常对照组10只和土三七实验组20只。土三七组给予土三七水煎液,18.18ml/kg/d,对照组给予等容积PBS,每日一次,连续28天,末次给药后,次日清晨各组大鼠给予安乐死。取大鼠血清,检测肝功能情况,留取肝组织行HE染色和MASSON染色,采用Deleve评分标准进行病理学评分。结果： 1.土三七组有18只大鼠成模,PBS对照组10只大鼠均未诱导出HSOS。 2.与PBS正常对照组比较,土三七组大鼠肝指数、ALT、AST、TBIL均明显升高,ALB明显下降(P0.05) 3.根据修改后的Deleve评分标准,土三七组大鼠多为中-重度HSOS改变。结论：本实验成功建立了土三七水煎液致肝窦阻塞综合征大鼠模型,为进一步研究HSOS发病机制奠定了良好的实验基础。第二部分超声造影对土三七诱导的HSOS的诊断价值目的：将超声造影与定量分析技术相结合,应用于大鼠HSOS模型,讨论超声造影技术在HSOS中的诊断价值。方法：PBS组和土三七组大鼠连续灌胃28天后,进行超声造影检查,使用定量分析软件对造影图像进行分析,绘制时间-强度曲线,得出各项定量参数。采用t检验法进行统计学分析,对两组大鼠各项定量参数进行比较,以P0.05认为差异有统计学意义。结果： 1.超声造影图像中土三七组大鼠肝实质强化不均匀,可见多处小片状高增强区域,甚至出现大片无灌注区,整个肝脏呈“地图样”强化,门静脉造影剂充填缓慢,肝静脉强化减弱或不强化。 2.时间-强度曲线中各项定量参数分析显示,与对照组比较,实验组大鼠达峰时间(TTP)、峰值减半时间(T1/2)、曲线上升支斜率(K1)、曲线下降支斜率(K2)延长,峰值强度(PI)减小(P0.05),其余参数如强度增量(PI-BI)、曲线下面积(AREA)两组间差异均无统计学意义(P0.05)。结论：超声造影与定量分析技术相结合,对HSOS的诊断具有一定的意义。超声造影有望成为无创伤性诊断HSOS的新指标。
[Abstract]:The first part: establishment and evaluation of HSOS model induced by Panax notoginseng in rats Objective: to establish a stable, replicable and effective HSOS model of rats with PBS- and Panax notoginseng decoction to simulate the drug taking by clinical patients, and to give a comprehensive evaluation. Methods 30 healthy male Sprague-Dawley rats were randomly divided into two groups: control group (n = 10) and control group (n = 20). The rats in the control group were treated with isovolumetric PBSs once a day for 28 days. The rats in each group were euthanized the next morning after the last administration. The liver function was detected in the serum of rats. The liver tissues were stained with HE and MASSON. The pathological scores were evaluated by Deleve scoring standard. Results: 1. No HSOSs were induced in 18 rats of PBS group and 10 rats in control group. 2. Compared with the normal control group of PBS, the liver index of rats in Panax notoginseng group was significantly higher than that in the control group (P 0.05). 3. According to the revised Deleve score, moderate to severe HSOS changes were found in rats of Panax notoginseng group. Conclusion: the rat model of hepatic sinusoidal obstruction syndrome induced by water decoction of Panax notoginseng was successfully established in this experiment, which laid a good experimental foundation for further study on the pathogenesis of HSOS. The value of contrast-enhanced ultrasonography in the diagnosis of HSOS induced by Panax notoginseng Objective: to study the diagnostic value of contrast-enhanced ultrasound (CEUs) in HSOS in rat HSOS model by combining contrast-enhanced ultrasound with quantitative analysis. Methods after 28 days of continuous gavage in the two groups, ultrasound examination was performed, and quantitative analysis software was used to analyze the contrast images. The time-intensity curves were drawn and the quantitative parameters were obtained. T test method was used to carry on the statistical analysis, the two groups of rats were compared with each other quantitative parameters, with P0.05 that the difference was statistically significant. Results: 1. The enhancement of hepatic parenchyma was not uniform in the contrast-enhanced images of the rats in the group of Notoginseng. There were many small and high enhancement areas, even a large area without perfusion. The whole liver was enhanced like a map, and the portal vein contrast agent was filled slowly. Hepatic vein enhancement was weakened or not. 2. The quantitative parameter analysis in the time-intensity curve showed that compared with the control group, the rats in the experimental group had the highest peak time of TTPN, the peak half time of T1 / 2, the slope of the ascending branch of the curve, the slope of the descending branch of the curve, and the slope of the descending branch of the curve (K2). Peak intensity (Pi) decreased P0.05N, but there was no significant difference between the two groups in other parameters, such as intensity increment PI-BII, area under curve area (AREAA), between the two groups, there was no significant difference between the two groups (P 0.05). Conclusion: the combination of contrast-enhanced ultrasound and quantitative analysis is of significance for the diagnosis of HSOS. Contrast-enhanced ultrasonography is expected to be a new index for non-traumatic diagnosis of HSOS.
【学位授予单位】：中南大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R445.1;R575

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