相变型纳米粒联合低强度聚焦超声体外显影及肿瘤治疗的实验研究

发布时间：2018-05-10 05:01

  本文选题：纳米粒 + 液态氟碳　； 参考：《重庆医科大学》2014年硕士论文

【摘要】：第一部分液态氟碳高分子纳米粒体外相变及超声显影的实验研究 目的制备一种新型纳米级超声造影剂—液态氟碳相变型高分子纳米粒，观察其物理特性、体外热致相变、声致相变及超声显影效果。 方法采用乳化—蒸发法（单乳化法）制备包裹液态氟碳（PFP）的MPEG-PLGA纳米粒（P-NP），光镜观察纳米粒形态分布;检测粒径和电位；在显微镜上放置加热板，观察P-NP的相变情况；体外行低强度聚焦超声（low intensity focused ultrasound, LIFU）仪辐照后，观察其超声显影效果。 结果所制备的包裹PFP的高子纳米粒外观为乳白色混悬液，形态规则，呈球形；平均粒径为（393.2±40.7）nm，平均电位为（－5.23±8.69）mV；加热板显示在42.6℃左右时，光镜下显示纳米粒开始转变为微气泡，且随着温度的增高，所产生的气泡逐渐增多；体外行LIFU仪辐照后，，在超声基波和谐波模式下可观察到显影明显增强。 结论成功制备的液态氟碳高分子纳米粒粒径小，稳定性好，体外经LIFU仪辐照后可增强超声显影，其有望成为一种新型的超声造影剂。 第二部分载药相变型纳米粒联合低强度聚焦超声治疗乳腺癌的体外实验研究 目的研究载紫杉醇相变型纳米造影剂联合低强度聚焦超声在辅助化疗方面的应用。 方法采用单乳化法制备在紫杉醇相变型纳米粒，以扫描及透射电镜观察其形态分布；在显微镜下观察热相变；于体外应用LIFU辐照后观察声相变的超声显影；高效液相色谱检测载药量、包封率及体外药物缓释；台盼蓝检测LIFU对MCF-7细胞的杀伤作用；CCK-8实验评估细胞增殖率。 结果扫描电镜PT-NP呈球形，大小较均匀，表面孔洞样及毛刷样结构；透射电镜PT-NP呈球形，表面毛刷样结构明显；体外加热板43℃时，PT-NP开始相变；声功率密度0.78w/cm2辐照2min可使PT-NP相变并显影；PT-NP加LIFU辐照组在48h内体外药物缓释量最高；LIFU声功率密度0.78w/cm2辐照2min本身对细胞无明显毒性作用；体外PT-NP加LIFU辐照组对MCF-7细胞增殖抑制最明显。 结论成功制备了载药相变型纳米粒，联合LIFU辐照，在增强超声显影的同时可肿瘤靶向治疗。
[Abstract]:Experimental study on phase transition and ultrasonic imaging of liquid fluorocarbon polymer nanoparticles in vitro Aim to prepare liquid fluorocarbon phase change polymer nanoparticles as a new ultrasonic contrast agent, and to observe their physical properties, thermal phase transition in vitro, acoustic phase transition and ultrasonic imaging effect. Methods MPEG-PLGA nanoparticles coated with liquid fluorocarbon (PFP) were prepared by emulsification-evaporation method (single emulsification method). The morphologic distribution of the nanoparticles was observed by light microscope, the particle size and potential were detected, and the phase change of P-NP was observed by placing a heating plate on the microscope. The effect of low intensity focused ultrasound (LIFU) on ultrasound was observed after irradiation with low intensity focused ultrasound (LIFU) in vitro. Results the appearance of the high particle coated with PFP was milky white suspension, regular shape and spherical shape, the average particle size was 393.2 卤40.7 nm, the average potential was -5.23 卤8.69 mV.The heating plate showed that at about 42.6 鈩

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