超顺磁性纳米氧化铁颗粒标记胰腺癌细胞的MR分子成像

发布时间：2018-05-14 15:50

本文选题：磁共振成像 + 超顺磁性纳米氧化铁颗粒　；参考：《中国医学影像技术》2017年08期

【摘要】：目的探讨超顺磁性纳米氧化铁颗粒(SPION)靶向标记胰腺癌细胞(BxPC-3)行MR分子成像的可行性。方法制备黏蛋白1(MUC1)靶向修饰的探针MUC1-SPION(靶向组),并以牛血清蛋白(BSA)制备非靶向探针BSA-SPION(非靶向组)。采过噻唑蓝(MTT)法测试不同MUC1-SPION浓度下(铁浓度为0、6.25、12.50、25、50、100、200μg/ml)的细胞毒性。并分别于铁浓度为50、100、200μg/ml条件下,对靶向组及对照组与BxPC-3细胞共同孵育2h后的细胞悬液进行MR成像,测定横向弛豫时间(T2),计算T2强化率。以普鲁士蓝染色观察靶向探针与细胞结合情况。结果 MTT法细胞毒性实验显示,不同MUC1-SPION浓度下BXPC-3细胞增殖率差异无统计学意义(F=1.74,P=0.183)。铁浓度50、100、200μg/ml条件下,2组间T2值和T2强化率的差异均有统计学意义(P均0.05)。普鲁士蓝染色显示靶向组的蓝染颗粒更多。结论 MUC1-SPION对BxPC-3细胞具有良好的靶向性,以SPION靶向标记BxPC-3细胞进行MRI安全、可行。
[Abstract]:Objective to investigate the feasibility of Mr molecular imaging of superparamagnetic nanocrystalline iron oxide (SPION) targeting pancreatic cancer cell line BxPC-3. Methods MUC1-SPION (target group) and BSA-SPION (non-target group) were prepared. The cytotoxicity of different concentrations of MUC1-SPION (Fe = 0.6.25 ~ 12.50 ~ 2550g / ml) was measured by using thiazolyl blue (MTT) method. At the concentration of 50100200 渭 g/ml, the suspension of BxPC-3 cells after incubating with the target group and the control group for 2 hours were examined by Mr imaging, and the transverse relaxation time and T _ 2 T _ 2 enhancement rate were measured and the T _ 2 enhancement rate was calculated. The binding of target probe to cells was observed by Prussian blue staining. Results MTT assay showed that there was no significant difference in the proliferation rate of BXPC-3 cells under different MUC1-SPION concentrations. Under the condition of 50100200 渭 g/ml iron concentration, there were significant differences in T2 value and T2 enhancement rate between the two groups (P < 0.05). Prussian blue staining showed that there were more blue staining particles in the target group. Conclusion MUC1-SPION has good targeting to BxPC-3 cells, and it is safe and feasible to label BxPC-3 cells with SPION.
【作者单位】：复旦大学附属浦东医院肝胆外科;
【基金】：上海市浦东新区科技发展基金医疗卫生项目(PKJ2015-Y33)
【分类号】：R445.2;R735.9

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