基于静息态功能磁共振功能连接正常人小脑脑老化研究

发布时间：2018-05-16 05:03

本文选题：静息态 + 功能磁共振成像　；参考：《河北医科大学》2017年硕士论文

【摘要】：目的:运用静息态功能磁共振成像(Resting-state functional magneti c resonance imaging,rs-fMRI)技术,采用基于体素的静息态功能连接(F unctional connectivity,FC)分析方法,研究中老年人和年轻人小脑各亚区之间功能连接模式,从而阐明小脑各亚区的功能连接模式是否存在年龄差异,以探讨正常脑老化对小脑功能连接的影响,了解小脑是否存脑认知功能及正常脑老化对小脑功能的影响。材料与方法:选择符合标准的正常志愿者共121人,均为右利手,分为两组:年轻组60人(男23人,女37人,年龄范围22-31岁,平均年龄26.15±1.9岁);中老年组61人(男23人,女38人,年龄范围45-65岁,平均年龄52.6±5.9岁)。采用GE公司Signa Excite 3.0T超导型磁共振对所有被试者行静息态fMRI扫描。应用基于MATLAB平台的DPARSFA、SPM8及REST软件进行预处理、功能连接计算及统计分析。预处理过程包括时间校正、头动校正、空间标准化、空间平滑、去线性漂移、去除协变量和低频滤波。在AAL模板中小脑共包含26个亚区,将其作为感兴趣区(ROI)分别进行功能连接分析,得到功能连接图。统计分析采用两样本T检验,观察中老年人和年轻人静息态状态下的功能连接模是否存在差异(P0.01),经过alphasim校正(rmm=5)。将经过两样本T检验的功能连接图分别叠加到Ch2bet结构模板进行显示,应用REST软件观察有统计学意义的激活团块大小、包含脑区、MNI峰值坐标、团块大小,并观察其T值。结果:1中老年人组与年轻人组比较功能连接减弱的脑区:1.1小脑蚓部1、2区(ROI1)中老年组较年轻组功能连接减弱的脑区:双侧中央前回、双侧中央后回、双侧楔叶、双侧颞上回、双侧舌回、双侧枕中回、双侧枕下回、左侧枕上回、左侧梭状回、右侧岛叶;1.2小脑蚓部3区(ROI2)中老年组较年轻组功能连接减弱的区域有:双侧枕中回、右侧枕下回、左侧舌回;1.3小脑蚓部4、5区(ROI3)中老年组较年轻组功能连接减弱的脑区:双侧中央后回、右侧中央前回、左侧眶部额上回、左侧内侧额上回、左侧背外侧额上回、右侧背外侧额上回;1.4小脑蚓部6区(ROI4)中老年组较年轻组功能连接减弱的脑区:右侧背外侧额上回、左侧颞下回、左侧直回、左侧楔前叶;1.5小脑蚓部7区(ROI5)中老年组较年轻组功能连接减弱的脑区:右侧中央后回、双侧内侧额上回、双侧眶部额中回;1.6小脑蚓部8区(ROI6)中老年组较年轻组功能连接减弱的脑区:双侧中央后回、双侧矩状裂周围部分皮质、双侧眶部额中回、右侧岛盖部额下回、右侧颞下回、右侧梭状回、左侧枕下回;1.7小脑蚓部9区(R0I7)中老年组较年轻组功能连接减弱的脑区:双侧中央后回、双侧中央前回、双侧矩状裂周围皮质、左侧直回、左侧舌回、左侧梭状回、右侧颞中回;1.8小脑蚓部10区(R0I8)中老年组较年轻组功能连接减弱的脑区:双侧中央后回、右侧中央前回、双侧枕上回、双侧枕下回、双侧眶部额上回、双侧梭状回、右侧颞下回、右侧颞中回;1.9左侧小脑3区(ROI9)中老年组较年轻组功能连接减弱的脑区:双侧中央前回、双侧中央后回、双侧枕上回、双侧枕中回、双侧枕下回、双侧楔叶、双侧梭状回、双侧颞中回、右侧颞下回、右侧额中回、左侧额上回、左侧额中回、左侧舌回、左侧顶下缘角回;1.10右侧小脑3区(ROI10)中老年组较年轻组功能连接减弱的脑区:双侧中央前回、双侧中央后回、双侧舌回、双侧楔叶、双侧颞中回、双侧颞下回、双侧梭状回、双侧枕上回、双侧枕中回、双侧枕下回、双侧岛叶、右侧内侧额上回、中脑左侧、右侧补充活动区;1.11左侧小脑4、5 区(ROI11)中老年组较年轻组功能连接减弱的脑区:右侧中央前回、右侧中央后回、右侧岛盖下额下回、右侧内侧额上回、右侧海马、左侧岛叶、左侧颞上回;1.12右侧小脑4、5区(ROI12)中老年组较年轻组功能连接减弱的脑区:双侧角回、左侧额中回、右侧内侧额上回、右侧眶部额下回;1.13左侧小脑6区(ROI13)中老年组较年轻组功能连接减弱的脑区:左侧颞上回颞极、双侧岛叶、右侧海马、右侧额上回、右侧丘脑、右侧扣带回前部;1.14右侧小脑6区(ROI14)中老年组较年轻组功能连接减弱的脑区:双侧海马、左侧海马旁回、左侧楔叶、左侧中央前回、左侧中央后回、左侧岛盖部额下回、左侧三角部额下回、右侧颞中回、右侧中央后回;1.15左侧小脑7b区(R0I15)中老年组较年轻组功能连接减弱的脑区:胼胝体、双侧扣带回前部、双侧梭状回、双侧海马旁回、左侧直回、左侧眶部额下回、右侧内侧额上回、右侧眶部额中回;1.16右侧小脑7b区(R0I16)中老年组较年轻组功能连接减弱的脑区:左侧海马旁回、左侧梭状回、左侧颞上回颞极、左侧颞中回颞极、右侧内侧额上回、胼胝体;1.17左侧小脑8区(R0I17)中老年组较年轻组功能连接减弱的脑区:脑干、双侧梭状回、双侧矩状裂周围皮质、右侧岛叶、左侧眶部额上回;1.18右侧小脑8区(ROI18)中老年组较年轻组功能连接减弱的脑区:脑干、延髓、左侧丘脑、左侧豆状核、胼胝体、右侧岛叶、右侧枕中回、左侧中央钩盖;1.19左侧小脑9区(R0I19)中老年组较年轻组功能连接减弱的脑区:右侧海马旁回、右侧枕中回;1.20右侧小脑9区(ROI20)中老年组较年轻组功能连接减弱的脑区:双侧内侧额上回、右侧颞上回、左侧矩状裂周围部分皮质;1.21左侧小脑10区(ROI21)中老年组较年轻组功能连接减弱的脑区:右侧中央后回、右侧眶部额上回、右侧颞横回、右侧中央沟盖、左侧直回、双侧岛叶;1.22右侧小脑10区(ROI22)中老年组较年轻组功能连接减弱的脑区:双侧中央后回、右侧中央前回、双侧岛叶、左侧颞上回、右侧梭状回、左侧舌回、右侧楔叶、右侧枕上回、右侧枕中回、右侧枕下回、右侧矩状裂周围部分皮质、左侧枕上回、左侧丘脑、左侧壳核;1.23左侧小脑脚1区(ROI23)中老年组较年轻组功能连接减弱的脑区:双侧中央前回、双侧中央后回、脑干偏左侧、左侧丘脑、双侧颞上回、左侧外侧额上回、左侧岛叶、右侧额中回、双侧扣带回中部、双侧补充活动区;1.24右侧小脑脚1区(ROI24)中老年组较年轻组功能连接减弱的脑区:脑干偏左侧、左侧丘脑、双侧豆状核、壳核、双侧中央后回、右侧中央沟盖、双侧扣带回中部;1.25左侧小脑脚2区(ROI25)中老年组较年轻组功能连接减弱的脑区:左侧额中回、右侧海马旁回、右侧梭状回、脑干、左侧丘脑;1.26右侧小脑脚2区(ROI26)中老年组较年轻组功能连接减弱的脑区:左侧颞上回、右侧舌回、右侧海马旁回。2中老年人组与年轻人组比较,小脑各亚区与大脑功能连接增强的脑区主要分布在额中回、额下回、颞中回、颞下回、顶下缘角回、缘上回及角回。结论:1在静息态下,中老年人组与年轻人比较小脑功能连接减弱的脑区范围较广泛,几乎涉所有静息态网络,而不是仅仅位于感觉运动网络;2中老年人组与年轻人组比较小脑功能连接减弱的脑区部分属于"大脑-小脑"回路;3正常脑老化对左、右侧小脑半球功能连接影响具有不对称性;4中老年人组与年轻人组比较存在小脑与大脑功能连接增强脑区,可能是对功能连接减弱相关脑区功能补偿的结果。
[Abstract]:Objective: To study the functions of the cerebellar subregions by using the resting state function magnetic resonance imaging (Resting-state functional magneti c resonance imaging, rs-fMRI) and the resting state function connection based on the voxel (F unctional connectivity, FC) method to study the functional connection patterns between the middle and the aged and the young cerebellum subregions. The effect of normal brain aging on cerebellar functional connection and the effect of brain cognitive function on cerebellum and the effect of normal aging on cerebellum function. Materials and methods: 121 normal volunteers were selected as right hand and were divided into two groups: 60 young men (23 men and 37 women). The age range of 22-31 years, the average age of 26.15 + 1.9 years, 61 people in the middle and old age group (23 men, 38 women, age 45-65, average age 52.6 + 5.9 years old). GE company Signa Excite 3.0T superconducting magnetic resonance (MRI) for all subjects resting state fMRI scan. MATLAB platform based DPARSFA, SPM8 and REST software for preprocessing, functional connectivity The preprocessing process includes time correction, head motion correction, space standardization, spatial smoothing, de linear drift, removal of covariance and low-frequency filtering. In the AAL template, the small brain consists of 26 sub regions, and the functional connection analysis is carried out as a region of interest (ROI), and a functional connection diagram is obtained. Statistical analysis uses two samples. T test was used to observe the difference between the resting state of the elderly and the young people in the resting state (P0.01), and the alphasim correction (rmm=5). The functional connection charts after two samples T test were superimposed to the Ch2bet structure template, and the REST software was used to observe the significant size of the activated mass, including the brain area, MNI peak. Value coordinates, mass size, and observe its T value. Results: 1 the elderly group and the young group compared the functional connection of the brain area: 1.1 the aged group (ROI1) in the 1.1 cerebellar vermis (ROI1) is more than the younger group's functional connection in the brain area: bilateral central anterior gyrus, bilateral central posterior gyrus, bilateral cuneate lobe, bilateral superior temporal gyrus, bilateral lingual gyrus, bilateral occipital gyrus, bilateral occipital Gyrus, left occipital gyrus, left fusiform gyrus, right Island leaf; 1.2 middle aged group (ROI2) in the 3 area of cerebellar vermis: bilateral occipital gyrus, right occipital gyrus, left lingual gyrus, and 1.3 cerebellar vermis 4,5 zone (ROI3) in older group than younger group: bilateral central posterior gyrus, right anterior central gyrus, left side The upper frontal gyrus of the orbit, the upper lateral frontal gyrus, the upper lateral frontal and lateral frontal gyrus, the right dorsal lateral frontal gyrus, and the 1.4 middle aged group (ROI4) in the 6 cerebellar vermis were compared with the younger group's functional connectivity: the right dorsolateral upper lateral frontal gyrus, left temporal gyrus, left left direct gyrus, and left anterior wedge lobe, and 1.5 cerebellar vermis (ROI5) in the middle aged group of 1.5 cerebellar vermis. The weak brain area: right posterior central gyrus, bilateral medial frontal gyrus, bilateral orbital frontal gyrus, and bilateral orbital frontal gyrus; 1.6 middle aged group (ROI6) in the 8 cerebellar vermis, compared with the younger group, the functional connection weakened in the brain: bilateral central posterior gyrus, bilateral partial cortex, bilateral orbital frontal gyrus, right inferior frontal gyrus, right inferior temporal gyrus, right fusiform gyrus, left occipital left 1.7 in the 9 region of the cerebellar vermis (R0I7) in the middle and old age group, the functional connection of the young group was less than that of the younger group: bilateral central posterior gyrus, bilateral central anterior gyrus, bilateral medial cortex, left straight gyrus, left lingual gyrus, left fusiform gyrus, right middle temporal gyrus, and 1.8 cerebellar vermis (R0I8) in the older group than the younger group. Back, right anterior central gyrus, bilateral occipital gyrus, bilateral occipital gyrus, bilateral orbital frontal gyrus, bilateral orbital frontal gyrus, bilateral spindle gyrus, right inferior temporal gyrus, right temporal gyrus, 1.9 left cerebellum 3 region (ROI9) older group than young group's functional connection weakened brain area: bilateral central anterior gyrus, bilateral central posterior gyrus, bilateral occipital gyrus, bilateral occipital gyrus, bilateral and inferior occipcipital gyrus, bilateral Cun Leaf, bilateral spindle gyrus, bilateral temporal gyrus, right temporal gyrus, right frontal gyrus, left superior frontal gyrus, left frontal gyrus, left lateral frontal gyrus, left lingual gyrus, and left apical angle gyrus; 1.10 right cerebellum 3 region (ROI10) aged group were compared with young group's functional connection weakened brain area: bilateral central anterior gyrus, bilateral central posterior gyrus, bilateral lingual gyrus, bilateral cuneate lobe, bilateral cuneate lobe, bilateral temporal gyrus Lateral temporal gyrus, bilateral spindle gyrus, bilateral occipital gyrus, bilateral occipital gyrus, bilateral occipital gyrus, bilateral occipital gyrus, bilateral insula, right medial frontal gyrus, left side of the middle brain, right supplementation area; 1.11 left cerebellar 4,5 area (ROI11) middle aged group compared with the younger group, the functional connection weakened in the brain area: right lateral central anterior gyrus, right posterior central gyrus, right Island inferior frontal and inferior gyrus, right island under the right side The medial frontal gyrus, right hippocampal, left insula, left temporal gyrus, 1.12 right cerebellar 4,5 area (ROI12) middle aged group were compared with the younger group's functional connectivity: bilateral angular gyrus, left middle frontal gyrus, right medial upper frontal gyrus, right orbital inferior frontal gyrus, and 1.13 left cerebellum 6 areas (ROI13) middle aged group compared with the younger group functional connection weakened brain area: left left brain area: left left brain area: left left brain area: left side: left side: left side: left side: left side: left side: left side: left side: left side: left side: left side: left side: left side: left side: left side: left side: left side: left side: left side: left side: left side: left side: left side: left side: left side: left side: left side: left side: left side: left side: left side: left side: left side: left side The upper temporal and temporal pole, bilateral Island, right hippocampus, right frontal gyrus, right frontal gyrus, right thalamus, right cingulate gyrus, and 1.14 right cerebellum 6 regions (ROI14) were compared with the younger group's functional connection in the brain: bilateral hippocampus, left parahippocampal gyrus, left cuneate lobe, left central anterior gyrus, left central posterior gyrus, left islid inferior frontal gyrus, left triangle frontal The lower right temporal gyrus, right posterior central posterior gyrus, 1.15 left cerebellar 7b area (R0I15) in the elderly group were compared with the younger group, the corpus callosum, bilateral cingulate gyrus, bilateral fusiform gyrus, bilateral parahippocampal gyrus, left side straight gyrus, left orbital frontal gyrus, right medial frontal gyrus, right orbital frontal gyrus, and right cerebellar 7b zone (R0I16), 1.16 In the older group, the functional connection weakened in the younger group: the left parahippocampal gyrus, the left fusiform gyrus, the left temporal gyrus and the temporal pole, the left temporal and temporal pole, the right medial frontal gyrus, the corpus callosum; the 1.17 middle aged group of the left cerebellum (R0I17) were compared with the younger group, the brain stem, the bilateral spindle gyrus, the bilateral fissure peri cortex, the right island. Leaves, left orbital frontal gyrus, 1.18 right cerebellum 8 region (ROI18) older group than the younger group functional connection weakened brain area: brain stem, medulla, left thalamus, left lenticular nucleus, corpus callosum, right Isle lobe, right occipital gyrus, left central hook lid, 1.19 left cerebellum 9 region (R0I19) older group than the younger group functional connection weakened brain area: right hippocampus hippocampus Paraplastic gyrus, right occipital gyrus, 1.20 right cerebellum 9 area (ROI20) older group than young group functional connection weakened brain area: bilateral medial upper frontal gyrus, right superior temporal gyrus, left moment split peripheral cortex; 1.21 left cerebellum 10 region (ROI21) middle aged group than young group function connected brain area: right posterior central gyrus, right orbital upper frontal gyrus, right Lateral temporal transverse gyrus, right central sulcus cover, left straight gyrus, bilateral insula, and 1.22 right cerebellum 10 areas (ROI22) in the middle and old age group compared with the younger group, the functional connection weakened in the brain: bilateral central posterior gyrus, right anterior central gyrus, bilateral insula, left lateral temporal gyrus, right fusiform gyrus, left lingual gyrus, right cuneate, right occipital gyrus, right occipital gyrus, right and right occipcipcipital gyrus, right The peripheral part cortex, left superior occipital gyrus, left thalamus, left putamen, 1.23 left cerebellar foot 1 (ROI23) middle aged group were compared with the younger group's functional connected brain area: bilateral central anterior gyrus, bilateral central posterior gyrus, left side of brain stem, left thalamus, bilateral superior temporal gyrus, left lateral frontal gyrus, left lateral insula, right middle frontal gyrus, bilateral buckle. 1.24 right cerebellar foot 1 areas (ROI24) in the right cerebellar foot 1 (ROI24) group had less functional connectivity than the younger group: the left side of the brain stem, the left thalamus, the bilateral lenticular nucleus, the putamen, the bilateral central posterior gyrus, the right central sulcus cover, the bilateral cingulate gyrus, and the 1.25 left cerebellar foot 2 (ROI25) middle aged group than the younger group. The weak brain area: left middle frontal gyrus, right parahippocampal gyrus, right fusiform gyrus, brain stem, left thalamus, 1.26 right cerebellar foot 2 (ROI26) middle aged group compared with young group functional connection weakened brain area: left temporal gyrus, right tongue gyrus, right hippocampal gyrus.2 middle aged group compared with young group, cerebellar subregions and brain functional connections were enhanced The brain region mainly distributed in the middle frontal gyrus, the lower frontal gyrus, the middle temporal gyrus, the inferior temporal gyrus, the inferior Cape gyrus, the upper margin and the angular gyrus. Conclusion: 1 in the resting state, the cerebellar functional connection of the middle-aged and the elderly compared with the young people is more extensive, almost all the resting networks, not only the sensory network; the elderly group and the young are younger than the young. The part of the brain region that the cerebellar functional connection is weaker than the cerebellar part belongs to the "cerebro cerebellum" loop, and the 3 normal brain aging has an asymmetric effect on the left and right cerebellar hemispheric functional connections; 4 in the middle and old age group, the cerebellar and brain functional connections are found to enhance the brain area, and can be compensatory for functional connection to decrease the function of the brain area. Result.

【学位授予单位】：河北医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R445.2

【参考文献】