苯肾上腺素对大鼠成骨细胞放射损伤的保护作用

发布时间：2018-05-24 12:11

本文选题：成骨细胞 + 电离辐射　；参考：《吉林大学学报(医学版)》2015年06期

【摘要】：目的:探讨苯肾上腺素对放射损伤大鼠成骨细胞的细胞形态以及烟酰胺磷酸核糖转移酶(Nampt)mRNA表达水平的影响,阐明苯肾上腺素对其可能的保护作用机制。方法:组织块法培养大鼠颅骨成骨细胞,随机分为空白对照组、苯肾上腺素组、单纯照射组和苯肾上腺素+照射组。苯肾上腺素组在照射前0.5h以100mmol·L-1苯肾上腺素孵育成骨细胞,照射组给予成骨细胞8Gy X射线照射。照射后8、16、32和64h在倒置显微镜下观察并分别收集细胞,提取细胞总RNA,应用RT-PCR法检测成骨细胞Nampt mRNA表达水平。结果:倒置显微镜下,与空白对照组比较,苯肾上腺素组成骨细胞形态、数量未见明显改变;单纯照射组细胞形态发生明显皱缩,尤以8h明显,细胞数量在8、16、32和64h时分别是对照组的0.82、0.37、0.24和0.21倍;苯肾上腺素+照射组在照射8和16h后细胞皱缩,32h后其形态恢复,在8、16、32和64h后其数量分别为空白对照组的0.91、0.83、0.72和0.75倍,是单纯照射组的1.09、2.24、3.00和3.60倍。RT-PCR法检测,在照射8、16、32和64h后,单纯照射组、苯肾上腺素+照射组Nampt mRNA表达水平较空白组明显减少(P0.01),苯肾上腺素+照射组较单纯照射组明显增加(P0.01)。结论:苯肾上腺素可减轻电离辐射导致的大鼠成骨细胞萎缩,并上调Nampt mRNA表达水平,其对成骨细胞放射损伤的保护作用可能与上调Nampt mRNA表达水平有关。
[Abstract]:Aim: to investigate the effects of phenylephrine on the morphology of osteoblasts and the expression level of niacin phosphonucleotransferase (Namptn) mRNA in rat osteoblasts after radiation injury, and to elucidate the possible protective mechanism of phenylephrine. Methods: osteoblasts of rat skull were cultured by tissue block method. They were randomly divided into three groups: control group, phenylephrine group, pure irradiation group and phenylepinephrine irradiation group. The osteoblasts were incubated with 100mmol L-1 phenylephrine 0.5 h before irradiation in the phenylephrine group, and the osteoblasts were irradiated with 8Gy X ray in the irradiation group. The cells were observed and collected under inverted microscope at 8h and 64h after irradiation. The total RNAs were extracted and the expression level of Nampt mRNA in osteoblasts was detected by RT-PCR method. Results: compared with the blank control group, the morphology of osteoblasts in phenylephrine group did not change obviously under inverted microscope, but the number of osteoblasts in pure irradiation group was obviously shrinked, especially at 8 hours. The number of cells was 0.82n0.37 0.24 and 0.21 times higher than that of the control group at 8h and 64h, respectively, and the morphology of the cells in phenylephrine group was recovered after 8 h and 16 h irradiation, and the number of cells in the group treated with phenylephrine was 0.910.30.72 and 0.75 times as much as that in the control group at 8h and 64h, respectively, and the number of cells in the group treated with phenylephrine was 0.910.30.72 and 0.75 times of that of the control group. Compared with the control group, the expression of Nampt mRNA in the pure irradiation group was significantly lower than that in the control group, and the expression level of Nampt mRNA in the benzene adrenaline irradiation group was significantly lower than that in the control group, and that in the phenylephrine group was significantly higher than that in the pure irradiation group after 816U 32 and 64 h. The expression of Nampt mRNA in the pure irradiation group was significantly lower than that in the control group, and that in the phenylephrine irradiation group was significantly higher than that in the pure irradiation group. Conclusion: phenylephrine can attenuate the atrophy of osteoblasts induced by ionizing radiation and up-regulate the expression of Nampt mRNA. The protective effect of phenylephrine on the radiation injury of osteoblasts may be related to the up-regulation of Nampt mRNA expression.
【作者单位】：大连大学医学院口腔医学系;大连医科大学附属第一医院口腔颌面外科;大连医科大学附属第二医院口腔颌面外科;
【基金】：国家自然科学基金资助课题(81170978,81371161) 辽宁省科技厅自然科学基金资助课题(201202008) 大连大学本科生创新教育基金项目资助课题(2013112)
【分类号】：R730.55

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