RGD靶向微泡造影剂的制备及其黏附效能

发布时间：2018-05-27 14:44

本文选题：超声检查 + 造影剂　；参考：《中国医学影像学杂志》2015年02期

【摘要】：目的制备一种新型的RGD超声微泡造影剂,探讨其在体外与小鼠血管内皮细胞(b End.3)靶向黏附效果,为肿瘤血管新生超声分子成像奠定基础。材料与方法以"生物素-亲和素"体系制备出RGD靶向微泡造影剂,分为10μg/ml RGD肽封闭组和30μg/ml RGD肽封闭组,未携带RGD肽微泡造影剂作为空白对照。粒径分析仪、普通光镜下进行表征或检测。体外培养小鼠血管内皮细胞,采用多肽封闭静态黏附实验验证靶向性和黏附效率;应用平行板流动腔芯片,设置不同流体剪切力,观察RGD靶向微泡造影剂在血管内皮细胞表面的动态黏附效果。结果测得的RGD靶向微泡粒径为(4.09±0.07)μm。单个细胞表面黏附的微泡个数RGD肽封闭组与RGD-MBs组比较,10μg/ml RGD肽封闭组为(2.98±0.35)个(P0.05),30μg/ml RGD肽封闭组为(1.78±0.23)个(P0.001)。10μg/ml和30μg/ml RGD肽封闭实验表明,黏附在单个细胞表面的微泡数量分别降低54.64%和67.00%。在剪切应力1.5 dyne/cm2下,RGD靶向微泡在细胞表面黏附率随着剪切应力增大而增加(P0.05)。当剪切应力为1.5 dyne/cm2时,RGD靶向微泡在细胞表面黏附率为(48.72±4.26)个,继续增大剪切应力,黏附率降低(P0.05)。结论 RGD靶向微泡造影剂能特异性地黏附血管内皮细胞,有望作为超声分子探针检测评价整合素ανβ3在肿瘤血管新生中的表达,用于肿瘤的早期诊断及预后检测。
[Abstract]:Objective to prepare a new type of RGD ultrasound microbubble contrast agent, and to investigate the effect of its targeted adhesion to mouse vascular endothelial cells (BEnd.3) in vitro, so as to lay a foundation for tumor angiogenesis by ultrasound molecular imaging. Materials and methods RGD targeted microbubble contrast agents were prepared by "biotin-affinity" system. They were divided into 10 渭 g/ml RGD peptide blocking group and 30 渭 g/ml RGD peptide blocking group, without RGD peptide microbubble contrast agent as blank control. Particle size analyzer, ordinary light microscope to characterize or detect. Murine vascular endothelial cells were cultured in vitro to verify the targeting and adhesion efficiency by using polypeptide blocking static adhesion assay, and different fluid shear forces were set up by using parallel plate flow cavity chip. To observe the dynamic adhesion effect of RGD targeted microbubble contrast agent on the surface of vascular endothelial cells. Results the diameter of RGD targeted microbubbles was 4.09 卤0.07 渭 m. The number of microbubbles attached to the surface of single cell in the RGD peptide blocking group was 2.98 卤0.35 in the 10 渭 g/ml RGD peptide blocking group compared with that in the RGD-MBs group. The number of microbubbles in the 10 渭 g/ml RGD peptide blocking group was 1.78 卤0.23) P 0.001U 路10 渭 g/ml and 30 渭 g/ml RGD peptide blocking. The results showed that the number of microbubbles adhered to the surface of the single cell was reduced by 54.64% and 67.00%, respectively. At 1. 5 dyne/cm2 shear stress, the adhesion rate of RGD-targeted microbubbles on the cell surface increased with the increase of shear stress. When the shear stress was 1.5 dyne/cm2, the adhesion rate of RGD-targeted microbubbles on the cell surface was 48.72 卤4.26, which continued to increase and the adhesion rate decreased. Conclusion RGD targeted microbubble contrast agent can specifically adhere to vascular endothelial cells, and it is expected to be used as a molecular probe to evaluate the expression of integrin 伪谓尾 3 in tumor angiogenesis, and to be used for early diagnosis and prognosis detection of tumor.
【作者单位】：西南科技大学生命科学与工程学院;北京大学深圳医院;广西壮族自治区人民医院超声科;
【基金】：国家自然科学基金项目(81260223)
【分类号】：R445.1

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