顺铂致兔急性肾损伤肾脏血流灌注的超声造影研究

发布时间：2018-06-14 17:57

本文选题：急性肾损伤 + 兔　；参考：《中国超声医学杂志》2016年11期

【摘要】：目的探讨顺铂致兔急性肾损伤(AKI)肾脏血流灌注的变化规律。方法雄性新西兰大白兔40只,经耳缘静脉注射顺铂建立兔AKI模型,取样时间分别为6(T6)、12(T12)、24(T24)、36(T36)、48h(T48),据此随机分为五组,每组8只。分别于建模前后相应时间点进行肾脏超声造影检查,采用Q-lab软件分析肾皮质、髓质TIC曲线。取耳缘静脉血检测建模前、后BUN及SCr值,AKI诊断指标参照KDIGO标准。使用SPSS22.0进行数据分析,P0.05为差异具有统计学意义。实验结束后,取兔肾组织,常规石蜡切片,HE染色光镜观察。结果 T24、T36及T48组实验兔均发生AKI。建模后T12、T24、T36及T48组皮质RT,T24、T36及T48组皮质PI,T36及T48组皮质WIS、AUC较建模前具有显著差异(P0.05)。建模后髓质各造影参数较建模前均无显著差异(P0.05)。结论超声造影可灵敏地反映顺铂所致AKI早期肾皮质血流灌注的改变,RT在血BUN及SCr未达到AKI诊断标准前已有明显改变,PI、WIS及AUC的变化则出现较晚。
[Abstract]:Objective to investigate the changes of renal blood perfusion in acute renal injury (AKI) induced by cisplatin in rabbits. Methods A total of 40 male New Zealand white rabbits were injected with cisplatin via ear margin to establish AKI model in rabbits. The sampling time of AKI was 6o T6, T12, T12, T24, T36, T36 and T48, respectively. According to the above, they were randomly divided into five groups, 8 in each group. Renal contrast echocardiography was performed at the corresponding time points before and after modeling, and the TIC curves of renal cortex and medulla were analyzed by Q-lab software. Before the examination of auricular vein blood, the diagnostic indexes of bun and SCR were compared with KDIGO standard. SPSS 2 2.0 was used for data analysis. At the end of the experiment, the renal tissue of rabbits was taken and observed under light microscope with HE staining on routine paraffin sections. Results AKI was found in both T 24 T 36 and T 48 groups. After modeling, there were significant differences in the cortex of T12, T24, T36 and T48, and the WIS-AUC of the T48 and T48 groups, respectively. There was no significant difference in the parameters of medullary angiography after modeling compared with that before modeling (P 0.05). Conclusion Contrast-enhanced ultrasonography can sensitively reflect the changes of renal cortical perfusion in early AKI induced by cisplatin. Before bun and SCR did not meet the diagnostic criteria of AKI, the changes of Pi WIS and AUC appeared later than those before bun and SCR met the diagnostic criteria of AKI.
【作者单位】：安徽医科大学解放军306临床学院超声医学科;中国人民解放军第306医院超声医学科;
【分类号】：R445.1;R692.5

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