循环游离DNA在胸膜良恶性病变患者血浆中的表达及意义

发布时间：2018-06-19 05:47

本文选题：循环游离DNA + 胸膜恶性病变　；参考：《广东医学》2015年18期

【摘要】：目的探讨循环游离DNA(cf DNA)水平及其完整性在胸膜增厚和良恶性病变患者间是否存在差异。方法胸膜良性病变患者55例,胸膜恶性病变患者33例,采用实时定量荧光PCR(RT-PCR)技术测定血浆cf DNA ALU115和ALU247基因水平及完整性(ALU247/ALU115),采用流式荧光免疫法检测血浆糖类抗原125(CA125)、糖类抗原199(CA199)、癌胚抗原(CEA)水平,采用B超检测胸膜增厚程度。结果胸膜恶性病变患者较胸膜良性病变患者胸膜厚度增厚[(5.18±1.31)mm vs(4.38±0.76)mm,P0.05],血浆cf DNA ALU115也偏高[(0.30±0.16)ng/μL vs(0.20±0.15)ng/μL,P0.05),胸膜恶性病变患者血浆cf DNA的ALU115水平还与CA199相关。胸膜厚度4.5 mm(AUC=0.715)诊断胸膜恶性患者的敏感度是54.2%,特异度是81.0%;循环cf DNA ALU1150.220 5 ng/μL诊断胸膜恶性患者的敏感度是37.1%,特异度是61.5%;同时串联运用该两种指标时,敏感度是74.1%,特异度是78.2%。结论循环cf DNA定量检测单独或与B超检查联合应用有助于胸膜增厚疾病良、恶性病变的鉴别诊断。
[Abstract]:Objective to investigate the difference between pleural thickening and benign and malignant lesions in the level and integrity of circulating free DNA cf DNA. Methods 55 cases of pleural benign lesions and 33 cases of malignant pleural lesions were studied. The plasma cf DNA levels of ALU115 and ALU247 genes and their integrity were measured by real-time quantitative fluorescence PCR RT-PCR.The plasma glycogen 125CA125, carbohydrate antigen 1999CA199and carcinoembryonic antigen CEA were detected by flow cytometry, and pleural thickening was detected by B-ultrasound. Results the pleural thickness of the patients with malignant pleural lesions was thicker than that of the patients with benign pleural lesions [5.18 卤1.31)mm vs(4.38 卤0.76mm / mm P0.05], and the plasma cf DNA ALU115 was also higher [0.30 卤0.16)ng/ 渭 L vs(0.20 卤0.15)ng/ 渭 L P0.05U]. The level of plasma cf DNA was also correlated with CA199 in the patients with malignant pleural lesions. The sensitivity and specificity of circulating cf DNA ALU1150.220 5 ng/ L in the diagnosis of pleural malignancy were 37.1 and 61.5, respectively, and the sensitivity was 74.1 and the specificity was 78.2 when the two indexes were used in series. Conclusion quantitative detection of circulating cf DNA alone or in combination with ultrasonography is helpful to the differential diagnosis of benign and malignant pleural thickening diseases.
【作者单位】：皖南医学院弋矶山医院超声医学科;皖南医学院弋矶山医院检验科;
【分类号】：R734.3;R445.1

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