MR分子探针RGD-USPIO评价核苷组合对人肝癌细胞Bel-7402的作用

发布时间：2018-06-25 18:15

本文选题：精氨酸-甘氨酸-天冬氨酸 + 超微超顺磁性氧化铁　；参考：《中国医学影像技术》2017年08期

【摘要】：目的利用超微超顺磁性氧化铁(USPIO)标记的含有精氨酸—甘氨酸—天冬氨酸(RGD)序列的环肽探针(RGD-USPIO)评价核苷组合对人肝癌细胞Bel-7402的作用效果及抗肿瘤作用机制。方法将对数生长期的人肝癌细胞Bel-7402分为2组,实验组加入1mmol/L核苷组合,对照组加入1640培养液,共同培养48h后加入等量的RGD-USPIO培养6h,行体外MR成像并测量T2WI信号强度。提取实验组和对照组的细胞总RNA和总蛋白,通过实时荧光定量PCR及Western blot检测整合素α_νβ_3的表达情况。结果MR扫描显示对照组和实验组T2WI信号强度分别为241.05±15.36、997.35±42.83,差异有统计学意义(t=28.79,P0.01);实时荧光定量PCR结果显示实验组α_νβ_3 mRNA表达水平是对照组的(0.22±0.02)倍(t=4.50,P0.01);Western blot结果显示实验组蛋白条带较对照组浅,实验组肿瘤细胞整合素α_νβ_3的表达较对照组低(t=11.88,P0.01)。结论核苷组合通过抑制整合素配体—受体结合而发挥抗肿瘤作用,其抗肿瘤机制可能与诱导肿瘤细胞凋亡有关;MR分子探针RGD-USPIO可简便、准确地评价核苷组合对Bel-7402细胞的作用。
[Abstract]:Objective to evaluate the effect of nucleoside combination on human hepatoma cell line Bel-7402 and its antitumor mechanism using ultrafine superparamagnetic iron oxide (USPIO) labeled cyclic peptide probe (RGD-USPIO) containing arginine glycine aspartate (RGD) sequence. Methods Bel-7402 human hepatoma cells in logarithmic phase were divided into two groups. The experimental group was added with 1 mmol / L nucleoside, the control group was cultured in 1640 medium for 48 h, then the same amount of RGD-USPIO was cultured for 6 h. The signal intensity of T2WI was measured by Mr imaging in vitro. The total RNA and total protein were extracted from the experimental group and the control group. The expression of integrin 伪 _ v 尾 _ 3 was detected by real-time fluorescence quantitative PCR and Western blot. Results the signal intensity of T2WI in control group and experimental group was 241.05 卤15.36997.35 卤42.83, respectively, the difference was statistically significant (tr 28.79, P0.01), and the expression level of 伪 _ v 尾 3 mRNA in experimental group was (0.22 卤0.02) times higher than that in control group (t4.50P0.01), the results of real-time fluorescence quantitative PCR showed that the expression level of 伪 _ v 尾 3 mRNA in experimental group was 0.22 卤0.02 times of that in control group (t4.50P0.01). The bands were shallower than those in the control group. The expression of integrin 伪 _ v 尾 _ 3 in the experimental group was lower than that in the control group (t _ (11.88) P _ (0.01). Conclusion nucleoside combination plays an antitumor role by inhibiting integrin ligand-receptor binding, and its antitumor mechanism may be related to the induction of apoptosis of tumor cells. RGD-USPIO may be a simple and convenient molecular probe for evaluating the effect of nucleoside combination on Bel-7402 cells.
【作者单位】：潍坊医学院附属医院影像中心;潍坊医学院临床医学院;
【基金】：山东省自然科学基金(ZR2011HQ023、ZR2014HP008) 山东省卫生厅科技计划项目(2011QZ026) 山东省医药卫生科技发展计划项目(2013WS0294)
【分类号】：R445.2;R735.7

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