基于核磁共振的800m跑训练前后尿液代谢组学特征

发布时间：2018-07-04 20:42

本文选题：核磁共振 + 生物分子　；参考：《中国组织工程研究》2015年46期

【摘要】：背景:目前研究发现800 m跑的代谢特征不是十分明晰,为800 m的运动训练造成了很大的阻碍。目的:了解800 m跑项目的物质和能量代谢特征,提高800 m跑训练的科学化水平,验证基于核磁共振的尿液代谢组学技术在运动训练监测方面的可行性。方法:对某高校运动训练专业7名中跑2级运动员进行一次极限强度800 m跑,结合人体代谢数据库(HMDB)等数据库,对运动前、后的尿液进行代谢组学相关分析。结果与结论:OPLS-DA模型显示运动前和运动后样本的代谢模式呈现簇类分布。造成这一变化的特征性代谢物主要有亮氨酸、乳酸、肌酐、琥珀酸、次黄嘌呤、腺苷等代谢物。通过分析可以推断出800 m跑是以糖酵解供能为主、磷酸原供能和有氧氧化供能共同参与的运动项目,800 m跑后运动员氨基酸代谢、甲烷代谢等代谢过程较为旺盛,而且在800 m跑的过程中会出现一定程度的氧化应激。结果说明基于核磁共振的尿液代谢组学技术有无创性、灵敏度高、样品需求量少、处理简单等优势。
[Abstract]:Background: at present, it is found that the metabolic characteristics of 800m running are not very clear, which is a great obstacle for 800m sports training. Objective: to understand the characteristics of substance and energy metabolism in 800m running events, improve the scientific level of 800m running training, and verify the feasibility of urine metabonomics technology based on nuclear magnetic resonance (NMR) in monitoring exercise training. Methods: seven middle and second grade athletes in a certain university were run with the limit strength of 800 m, and the urine before and after exercise was analyzed with the database of Human body Metabolism Database (HMDB) and other databases. Results and conclusions the OPLS-DA model showed that the metabolic patterns of pre-exercise and post-exercise samples showed cluster distribution. The main metabolites responsible for this change are leucine, lactic acid, creatinine, succinic acid, Hypoxanthine, adenosine and other metabolites. It can be inferred from the analysis that the main metabolic process of 800 m running is glycolysis, the metabolism of amino acid, methane and so on after 800 m running. And there will be a certain degree of oxidative stress during the 800 m run. The results showed that the urinary metabolomics technique based on nuclear magnetic resonance had the advantages of noninvasive, high sensitivity, less sample demand and simple treatment.
【作者单位】：上海体育学院;
【基金】：上海市科学技术委员会科研计划项目(13490503700)《运动员不同类型运动性疲劳代谢组学图谱的建立及针灸调控》~~
【分类号】：R445.2;R87

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