恶性肿瘤患者放疗后人巨细胞病毒感染的研究

发布时间：2018-07-20 16:47

【摘要】：目的分析恶性肿瘤患者放疗后人巨细胞病毒(HCMV)感染的状况,以提高诊断率。方法选取2012年6月-2013年12月在医院就诊的106例肿瘤患者,其中53例经过放疗治疗为放疗组,53例未接受放疗的为未放疗组,另选取53名健康受试者为对照组,采用酶联免疫法检测血清中HCMV-IgM抗体的水平,同时用实时定量PCR方法检测HCMV-DNA的含量,比较3组HCMV感染率。结果放疗组、未放疗组和对照组受试者的HCMV-IgM抗体检出率分别为16.9%、9.4%、1.9%;放疗组与未放疗组患者血清中HCMV-IgM抗体的检出率差异无统计学意义,放疗组、未放疗组与对照组的抗体检出率差异有统计学意义(P0.05);放疗组、未放疗组和对照组患者的HCMV-DNA检出率分别为39.6%、22.6%和3.8%;放疗组与未放疗组患者及对照组血清中HCMV-DNA检出率的差异有统计学意义(P0.05),未放疗组患者与对照组比较差异也有统计学意义(P0.05);实时定量PCR方法检测HCMV活动性感染率的敏感性要高于酶联免疫法。结论肿瘤患者HCMV的感染率增高,放疗后HCMV的感染率进一步增加,实时定量PCR的方法检测HCMV感染率敏感性较高。
[Abstract]:Objective to analyze the status of human cytomegalovirus (HCMV) infection in patients with malignant tumor after radiotherapy. Methods A total of 106 tumor patients were selected from June 2012 to December 2013 in our hospital. Among them, 53 patients were treated with radiotherapy, 53 patients were not treated with radiotherapy, and 53 healthy subjects were selected as control group. The level of HCMV IgM antibody in serum was detected by enzyme-linked immunosorbent assay (Elisa), and the HCMV DNA content was detected by real-time quantitative PCR. The infection rate of HCMV in three groups was compared. Results the positive rates of HCMV-IgM antibody in the patients of radiotherapy group, non-radiotherapy group and control group were 16.9and 9.4, respectively, while there was no significant difference between radiotherapy group and non-radiotherapy group, and the detection rate of HCMV-IgM antibody in radiotherapy group was not significantly different from that in radiotherapy group, and there was no significant difference in HCMV-IgM antibody detection rate between radiotherapy group and non-radiotherapy group. The positive rate of antibody in non-radiotherapy group was significantly different from that in control group (P0.05). The positive rate of HCMV-DNA was 39.6% and 3.8% in non-radiotherapy group and control group respectively, the difference of HCMV-DNA detection rate between radiotherapy group and non-radiotherapy group and control group was statistically significant (P0.05), and there was also statistical difference between non-radiotherapy group and control group (P0.05). The sensitivity of real-time quantitative PCR was higher than that of enzyme-linked immunosorbent assay (Elisa) in detecting the active infection rate of HCMV. Conclusion the infection rate of HCMV in tumor patients is higher than that in patients after radiotherapy. The sensitivity of real-time quantitative PCR for detecting HCMV infection is higher.
【作者单位】：汉川市人民医院肿瘤科;汉川市人民医院儿科;汉川市人民医院眼科;
【基金】：湖北省卫生厅基金资助项目(WJ01564)
【分类号】：R730.55

【参考文献】