Wistar大鼠放射性腮腺损伤模型的建立

发布时间：2018-08-07 10:21

【摘要】：目的建立Wistar大鼠放射性腮腺损伤模型。方法选取成年Wistar大鼠25只,随机分为两组;实验组15只使用直线加速器照射,每次5 Gy,共40 Gy。对照组10只不做放射,正常饲养。观察两组实验动物的饮食体质量及腮腺区局部变化;照射完成4周后处死动物,并在光镜下观察腮腺组织病理改变。结果对照组腺体组织结构清晰。实验组大鼠腺体组织结构疏松,腺小叶间隙扩大;间质纤维组织增多,血管扩张,片状炎细胞浸润;以浆细胞为主,可见淋巴细胞;胞质浓缩,染色深,核缩小,深染;腮腺内毛细血管内皮增厚,血管肿胀,周围组织有明显纤维化;血管内皮增生,细胞呈梭形,向血管腔内突出。实验组毛细血管密度明显低于对照组。结论成功建立了Wistar大鼠放射性腮腺损伤模型。
[Abstract]:Objective to establish a model of radioactive parotid injury in Wistar rats. Methods 25 adult Wistar rats were selected and divided into two groups randomly. In the experimental group, 15 rats were irradiated with linear accelerator, 5 Gy each time, and 10 of the 40 Gy. control groups were not irradiated normally. The diet mass of the two experimental animals and the local changes of parotid region were observed, and the irradiation was completed for 4 weeks. The pathological changes of parotid gland were observed under light microscope. The tissue structure of the gland was clear in the control group. The gland tissue structure of the experimental group was loose and the glandular lobule space expanded; the interstitial fibrous tissue increased, the vascular dilatation, and the flaky inflammatory cells infiltrated; the plasma cells were mainly used to see the lymphocytes; the cytoplasm concentrated, dyed deep, nuclear shrinks, deep staining; gills; gills. The capillary endothelium in the gland was thickened, the blood vessels were swollen, the surrounding tissue had obvious fibrosis, the vascular endothelium was proliferated, the cells were spindle shaped, and the blood vessel density was prominent. The capillary density in the experimental group was significantly lower than that of the control group. Conclusion the radioactive parotid injury model of Wistar rats was successfully established.
【作者单位】：青岛市市立医院;
【基金】：山东省卫生计生委立项课题(2014WS2018) 青岛市卫生计生委立项课题(2008-wszd024;KJZD-13-15-NSH)
【分类号】：R-332;R730.55

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