经颅超声刺激在缺血性脑损伤中的保护研究
发布时间：2018-08-07 18:54
【摘要】：经颅超声刺激是新近发展起来的一种大脑功能刺激技术,该技术通过透过完整的颅骨传递超声能量，能无创地调制大脑的神经活动。本文首先介绍了超声在生物医学中的应用以及经颅超声刺激技术的研究现状及意义，，然后介绍了经颅超声刺激系统的关键参数以及提高空间分辨率的方法。建立经颅超声系统之后，首先通过经颅脉冲超声刺激SD大鼠的motor及Barrel皮层，研究不同的超声刺激参数调解大脑神经活动的能力；随后，基于中风模型建立之后立即给予超声刺激能够有效地对于保护缺血性脑损伤的假设，设计实验利用经颅超声刺激SD大鼠远端中动脉缺血性模型，通过动物行为学评定运动状况（刺激组(n=10,5.5±2.5)对照组(n=10,10.5±1.4)）、TTC染色测定皮层组织梗死面积比（刺激组(13.78%±7.39%)对照组(43.39%±2.33%)）、内源光成像技术评估脑功能等来评价经颅超声刺激对缺血性脑损伤的保护效果，最后通过激光散斑成像技术观测感兴趣区域的血供以及免疫组化测定细胞炎症来研究超声的脑保护的潜在作用机制，提出了血流灌注增强和炎症反应减弱两种可能的保护作用机制。本项目的研究有望揭示经颅超声刺激在卒中后的早期神经保护的作用机理，并为缺血性脑卒中的神经保护提供新的治疗康复方法。
[Abstract]:Transcranial ultrasound stimulation (TUS) is a newly developed technique of brain function stimulation, which can modulate the neural activity of the brain noninvasively by transmitting ultrasonic energy through the intact skull. In this paper, the application of ultrasound in biomedicine and the research status and significance of transcranial ultrasound stimulation are introduced, then the key parameters of transcranial ultrasound stimulation system and the method of improving spatial resolution are introduced. After the establishment of transcranial ultrasound system, the motor and Barrel cortex of SD rats were stimulated by transcranial pulse ultrasound first to study the ability of different ultrasound stimulation parameters to regulate the brain nerve activity. Based on the hypothesis that ultrasound stimulation can effectively protect ischemic brain injury immediately after the establishment of stroke model, we designed an ischemic model of distal middle artery of SD rats by transcranial ultrasound stimulation. The infarct area ratio of cortical tissue was measured by animal behavior assessment (stimulation group (10 5 卤2 5 卤2 5) control group (n 10 10 5 卤1 4) and control group (13 78% 卤7 39%) and control group (43 39% 卤2 33%), endogenous light imaging technique) to evaluate the effect of transcranial ultrasound stimulation on ischemic ischemia. The protective effect of brain injury, Finally, the potential mechanism of ultrasonic brain protection was studied by using laser speckle imaging technique to observe the blood supply in the region of interest and to determine the cellular inflammation by immunohistochemistry. Two possible protective mechanisms of increased blood perfusion and weakened inflammatory response were proposed. This project is expected to reveal the mechanism of transcranial ultrasound stimulation in early post-stroke neuroprotection and provide a new method for the treatment and rehabilitation of ischemic stroke.
【学位授予单位】：上海交通大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R445.1;R743.3
本文编号：2171035
[Abstract]:Transcranial ultrasound stimulation (TUS) is a newly developed technique of brain function stimulation, which can modulate the neural activity of the brain noninvasively by transmitting ultrasonic energy through the intact skull. In this paper, the application of ultrasound in biomedicine and the research status and significance of transcranial ultrasound stimulation are introduced, then the key parameters of transcranial ultrasound stimulation system and the method of improving spatial resolution are introduced. After the establishment of transcranial ultrasound system, the motor and Barrel cortex of SD rats were stimulated by transcranial pulse ultrasound first to study the ability of different ultrasound stimulation parameters to regulate the brain nerve activity. Based on the hypothesis that ultrasound stimulation can effectively protect ischemic brain injury immediately after the establishment of stroke model, we designed an ischemic model of distal middle artery of SD rats by transcranial ultrasound stimulation. The infarct area ratio of cortical tissue was measured by animal behavior assessment (stimulation group (10 5 卤2 5 卤2 5) control group (n 10 10 5 卤1 4) and control group (13 78% 卤7 39%) and control group (43 39% 卤2 33%), endogenous light imaging technique) to evaluate the effect of transcranial ultrasound stimulation on ischemic ischemia. The protective effect of brain injury, Finally, the potential mechanism of ultrasonic brain protection was studied by using laser speckle imaging technique to observe the blood supply in the region of interest and to determine the cellular inflammation by immunohistochemistry. Two possible protective mechanisms of increased blood perfusion and weakened inflammatory response were proposed. This project is expected to reveal the mechanism of transcranial ultrasound stimulation in early post-stroke neuroprotection and provide a new method for the treatment and rehabilitation of ischemic stroke.
【学位授予单位】：上海交通大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R445.1;R743.3
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