正常脑老化过程中双侧海马不同部位MRS差异性研究
发布时间：2018-08-23 14:42
【摘要】：目的：利用1H-MRS技术，评价正常脑老化过程中双侧海马不同部位物质代谢的变化情况，并进一步探讨代谢物浓度及代谢物浓度比值与年龄间的相关性，旨在探索海马的生理老化规律，为海马相关疾病的早期诊断及其治疗奠定基础。 方法：121名健康右利手志愿者，男62名，女59名，年龄20~89岁，平均48.7±16.5岁，行颅脑常规MRI、MRS检查，按年龄分为青年组（20~44岁）、中年组（45~59岁）、老年组（60~89岁），每组约40人，行颅脑常规MRI、MRS检查。人为确定感兴趣区，通过扫描，机器自动测量出所需参数：NAA、Cho、Cr浓度及NAA/Cr、Cho/Cr等浓度比值。应用SPSS13.0软件进行统计分析。采用t检验(分组、配对t检验)、方差分析(One-Way ANOVA)等统计方法分析不同性别、不同侧别、年龄组间各统计指标间的差异性，规定以P 0.05为差异有统计学意义，其中方差分析年龄组间差异性时两两年龄组间比较采用最小显著差异(least significantdifference, LSD)t检验。最后应用Pearson相关分析，进一步分析海马各部位代谢物NAA、Cho、Cr浓度与年龄的直线相关关系。 结果： 1双侧海马头、体（体1区、体2区）、尾部NAA浓度值与年龄存在负相关（P＜0.05）；双侧海马头、体（体1区、体2区）、尾部Cho、Cr浓度值与年龄不存在相关性(p＞0.05)。 2年龄组间差异性：双侧海马头、体（体1区、体2区）、尾部NAA老年组与青年组（P1-3）、中年组（P2-3）间存在统计学差异性，P＜0.05，且老年组明显低于青年组及中年组，而青年组与中年组间P＞0.05，无统计学学意义；Cho及Cr在青、中、老年三组间两两组间比较结果均表现为P＞0.05，无统计学意义。双侧海马头部代谢物浓度比NAA/Cr在老年组与青年组（P1-3）、老年组与中年组间（P2-3）存在差异性，P＜0.05，且老年组明显低于青年组及中年组，而青年组与中年组间P1-2＞0.05，，尚不能认为青年组与中年组间NAA/Cr存在差异。双侧海马体1区、左侧海马体2区、左侧海马尾部代谢物浓度比NAA/Cr结果显示仅P1-3＜0.05，存在统计学差异性。海马头、体（体1区、体2区）、尾各部位各年龄组间Cho、Cr、Cho/Cr结果均显示P＞0.05，无统计学意义。 3性别差异性：男性各代谢物质NAA、Cho、Cr及其代谢物比值NAA/Cr、Cho/Cr与女性相比未见明显统计学差异，p＞0.05。 4侧别差异性：海马头部NAA、Cho、Cr、NAA/Cr、Cho/Cr均不存在侧别差异（P＞0.05）；海马体1区NAA、Cho、Cr存在侧别差异性（P＜0.05）,且右侧大于左侧，而NAA/Cr、Cho/Cr不存在侧别差异（P＞0.05）；海马体2区NAA、Cho、Cr存在侧别差异性（P＜0.05），且右侧大于左侧，而NAA/Cr、Cho/Cr不存在侧别差异（P＞0.05)；海马尾部NAA、Cho、Cr存在侧别差异性（P＜0.05），且右侧大，而NAA/Cr、Cho/Cr不存在侧别差异（P＞0.05)。 结论： 1应用MRS评估海马代谢物质随年龄变化有无规律性是可行的。 2在海马各部位均发现老年组NAA明显减低，青年组与中年组间变化不大；而NAA/Cr结果与NAA结果存在差异性，证明NAA/Cr是NAA及Cr共同作用的结果，观测神经元及轴突变化时NAA较NAA/Cr更精准；海马同一部位不同年龄组间Cho、Cr、Cho/Cr不存在差异性。 3海马头、体（体1区、体2区）、尾部NAA值均随年龄的增高而降低；Cho、Cr与年龄无此线性关系。 4海马同一部位各代谢物及其代谢物浓度比值间均不存在性别差异性。 5海马体1、体2区、尾部代谢物浓度存在侧别差异，表现为右侧＞左侧，海马体1区、体2区、尾部代谢物浓度比值不存在侧别差异；海马头部代谢物浓度及代谢物浓度比值均不存在侧别差异。
[Abstract]:Objective: To evaluate the metabolic changes of different parts of bilateral hippocampus during normal brain aging by 1H-MRS, and to explore the correlation between metabolite concentration and metabolite concentration ratio and age, so as to explore the physiological aging regularity of hippocampus and lay a foundation for early diagnosis and treatment of hippocampal related diseases.
Methods: 121 healthy right-handed volunteers, 62 males and 59 females, aged from 20 to 89 years, with an average of 48.7 (+ 16.5 years) underwent routine MRI and MRS examinations. They were divided into young group (20-44 years old), middle-aged group (45-59 years old), and old group (60-89 years old). Routine MRI and MRS examinations were performed on 40 subjects in each group. SPSS13.0 software was used for statistical analysis. T test (grouping, paired t test), one-way ANOVA and other statistical methods were used to analyze the differences of statistical indicators among different gender, different side, and age groups. It was stipulated that P 0.05 was the difference of statistical indicators. Meaning, variance analysis was used to compare the differences between age groups by least significant difference (LSD) t test. Finally, Pearson correlation analysis was used to further analyze the linear correlation between the concentrations of NAA, Cho, Cr and age in different parts of the hippocampus.
Result:
There was a negative correlation between the concentration of NAA in the head, body (body 1 area, body 2 area), tail and age (P < 0.05); there was no correlation between the concentration of Cho and Cr in the head, body (body 1 area, body 2 area), tail and age (p > 0.05).
The differences between the two-year-old groups were statistically significant (P The results showed that there was no significant difference between the two groups (P > 0.05). The concentration ratio of NAA / Cr in hippocampus was different between the old group and the young group (P 1-3), the old group and the middle-aged group (P < 0.05), and the old group was significantly lower than the young group and the middle-aged group. There were significant differences in NAA/Cr between middle-aged and middle-aged groups.The concentrations of metabolites in bilateral hippocampus 1 area, left hippocampus 2 area and left hippocampus tail area were only P1-3 < 0.05. The results of Cho, Cr and Cho/Cr in hippocampus head, body (body 1 area, body 2 area) and tail area showed no statistical significance.
Gender differences: There was no significant difference in the ratio of NAA, Cho, Cr and their metabolites NAA/Cr, Cho/Cr between male and female, P > 0.05.
There were no lateral differences in NAA, Cho, Cr, NAA/Cr and Cho/Cr in hippocampus head (P > 0.05); there were lateral differences in NAA, Cho and Cr in hippocampus 1 region (P < 0.05), and the right side was larger than the left side, but there was no lateral difference in NAA/Cr and Cho/Cr in hippocampus 2 region (P > 0.05); there were lateral differences in NAA, Cho and Cr in hippocampus 2 region (P < 0.05), and the right side was larger than the left side, and the right side was larger than the left side. There was no lateral difference in NAA/Cr and Cho/Cr (P > 0.05), and there was lateral difference in NAA, Cho and Cr in the tail of hippocampus (P < 0.05), but there was no lateral difference in NAA/Cr and Cho/Cr (P > 0.05).
Conclusion:
1 it is feasible to use MRS to assess whether there is regularity in the metabolism of hippocampal metabolites with age.
2. The NAA decreased significantly in all parts of the hippocampus, and there was no significant difference between young and middle-aged groups. The NAA/Cr results showed that NAA/Cr was the result of the interaction of NAA and Cr. The opposite sex.
The NAA values of hippocampus head, body (body 1, body 2) and tail decreased with age, but Cho, Cr had no linear relationship with age.
4 there was no gender difference in the ratios of metabolites and metabolites in the same part of the hippocampus.
5 There were lateral differences in the concentrations of metabolites in hippocampus 1, body 2 and tail, showing that there were no lateral differences in the concentrations of metabolites in right side > left side, hippocampus 1, body 2 and tail, and there were no lateral differences in the concentrations of metabolites in head and tail of hippocampus.
【学位授予单位】：河北医科大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R338;R445.2
本文编号：2199377
[Abstract]:Objective: To evaluate the metabolic changes of different parts of bilateral hippocampus during normal brain aging by 1H-MRS, and to explore the correlation between metabolite concentration and metabolite concentration ratio and age, so as to explore the physiological aging regularity of hippocampus and lay a foundation for early diagnosis and treatment of hippocampal related diseases.
Methods: 121 healthy right-handed volunteers, 62 males and 59 females, aged from 20 to 89 years, with an average of 48.7 (+ 16.5 years) underwent routine MRI and MRS examinations. They were divided into young group (20-44 years old), middle-aged group (45-59 years old), and old group (60-89 years old). Routine MRI and MRS examinations were performed on 40 subjects in each group. SPSS13.0 software was used for statistical analysis. T test (grouping, paired t test), one-way ANOVA and other statistical methods were used to analyze the differences of statistical indicators among different gender, different side, and age groups. It was stipulated that P 0.05 was the difference of statistical indicators. Meaning, variance analysis was used to compare the differences between age groups by least significant difference (LSD) t test. Finally, Pearson correlation analysis was used to further analyze the linear correlation between the concentrations of NAA, Cho, Cr and age in different parts of the hippocampus.
Result:
There was a negative correlation between the concentration of NAA in the head, body (body 1 area, body 2 area), tail and age (P < 0.05); there was no correlation between the concentration of Cho and Cr in the head, body (body 1 area, body 2 area), tail and age (p > 0.05).
The differences between the two-year-old groups were statistically significant (P The results showed that there was no significant difference between the two groups (P > 0.05). The concentration ratio of NAA / Cr in hippocampus was different between the old group and the young group (P 1-3), the old group and the middle-aged group (P < 0.05), and the old group was significantly lower than the young group and the middle-aged group. There were significant differences in NAA/Cr between middle-aged and middle-aged groups.The concentrations of metabolites in bilateral hippocampus 1 area, left hippocampus 2 area and left hippocampus tail area were only P1-3 < 0.05. The results of Cho, Cr and Cho/Cr in hippocampus head, body (body 1 area, body 2 area) and tail area showed no statistical significance.
Gender differences: There was no significant difference in the ratio of NAA, Cho, Cr and their metabolites NAA/Cr, Cho/Cr between male and female, P > 0.05.
There were no lateral differences in NAA, Cho, Cr, NAA/Cr and Cho/Cr in hippocampus head (P > 0.05); there were lateral differences in NAA, Cho and Cr in hippocampus 1 region (P < 0.05), and the right side was larger than the left side, but there was no lateral difference in NAA/Cr and Cho/Cr in hippocampus 2 region (P > 0.05); there were lateral differences in NAA, Cho and Cr in hippocampus 2 region (P < 0.05), and the right side was larger than the left side, and the right side was larger than the left side. There was no lateral difference in NAA/Cr and Cho/Cr (P > 0.05), and there was lateral difference in NAA, Cho and Cr in the tail of hippocampus (P < 0.05), but there was no lateral difference in NAA/Cr and Cho/Cr (P > 0.05).
Conclusion:
1 it is feasible to use MRS to assess whether there is regularity in the metabolism of hippocampal metabolites with age.
2. The NAA decreased significantly in all parts of the hippocampus, and there was no significant difference between young and middle-aged groups. The NAA/Cr results showed that NAA/Cr was the result of the interaction of NAA and Cr. The opposite sex.
The NAA values of hippocampus head, body (body 1, body 2) and tail decreased with age, but Cho, Cr had no linear relationship with age.
4 there was no gender difference in the ratios of metabolites and metabolites in the same part of the hippocampus.
5 There were lateral differences in the concentrations of metabolites in hippocampus 1, body 2 and tail, showing that there were no lateral differences in the concentrations of metabolites in right side > left side, hippocampus 1, body 2 and tail, and there were no lateral differences in the concentrations of metabolites in head and tail of hippocampus.
【学位授予单位】：河北医科大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R338;R445.2
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