微泡增强超声空化联合血凝酶对兔肝局部微血管阻断作用的实验研究

发布时间：2018-08-24 20:50

【摘要】：目的探讨微泡增强超声空化联合血凝酶对兔肝局部微血管的损毁及阻断作用。方法 40只健康新西兰大白兔,随机分为生理盐水组、血凝酶组、微泡组和微泡联合血凝酶组(联合组)。各组均分别在实验前后行超声造影检查,使用专用图像处理系统分析各组实验前后造影的视觉图像及峰值灰阶值(GSV)的变化,并与病理改变进行对照。结果实验前后,生理盐水组和血凝酶组超声图像造影视觉改变及定量分析峰值GSV均无明显变化(P0.05)。微泡组和联合组视觉观察有造影灌注缺损,联合组更为明显;定量分析微泡组峰值GSV从(62.94±6.19)降至(36.36±7.30)(P0.05),联合组峰值GSV从(63.52±8.53)降至(22.91±5.39)(P0.05)。实验后生理盐水组和血凝酶组病理无明显改变;微泡组偶见局灶性小片状出血;联合组可见明显的细胞变性和坏死,伴有微血栓形成。结论微泡增强超声空化联合血凝酶可以造成更明显的兔肝局部微血管的损毁及血流阻断。
[Abstract]:Objective to investigate the effect of microbubble enhanced ultrasound cavitation combined with hemagglutinin on the damage and blockage of hepatic microvessels in rabbits. Methods 40 healthy New Zealand rabbits were randomly divided into normal saline group, hemagglutinin group, microbubble group and microbubble combined hemagglutinin group (combined group). Each group was examined by contrast-enhanced ultrasound before and after the experiment. The changes of visual image and peak gray scale (GSV) were analyzed by special image processing system before and after the experiment, and compared with pathological changes. Results before and after the experiment, there were no significant changes in visual changes and quantitative analysis of peak GSV in normal saline group and hemagglutinin group (P0.05). The visual perfusion defect was observed in the microbubble group and the combined group, especially in the combined group, the peak GSV of the microbubble group decreased from (62.94 卤6.19) to (36.36 卤7.30) (P0.05), the peak GSV of the combined group decreased from (63.52 卤8.53) to (22.91 卤5.39) (P0.05). The pathological changes were not obvious in saline group and hemagglutinin group; focal small hemorrhage was occasionally found in microbubble group; cell degeneration and necrosis with microthrombotic formation were observed in combined group. Conclusion Microbubble enhanced ultrasound cavitation combined with hemagglutinin can cause more obvious damage and occlusion of blood flow in rabbit liver.
【作者单位】：第四军医大学西京医院超声诊断科;第四军医大学西京医院麻醉科;
【基金】：国家自然科学基金面上项目(No.81371567)
【分类号】：R735.7;R445.1

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