超声心动图引导建立小型猪主动脉瓣反流模型的方法学研究

发布时间：2018-09-04 06:35

【摘要】：目的:研究如何应用超声心动图引导建立小型猪主动脉瓣反流模型。方法:选用中华小型猪,静脉全麻后测量体重,并行超声心动图检查,排除主动脉瓣病变。将实验猪颈部小切口,暴露颈动脉,置入鞘管后送导管至主动脉窦部,用硬导丝穿出导管,调整导管位置,造成主动脉瓣损伤。超声评估主动脉瓣损伤情况及反流量。处死动物,取心脏,观察主动脉瓣损害情况。结果:共入选中华小型猪7只,雄性4只,雌性3只,动物平均体重(24.7±3.6)kg。共5只顺利完成超声引导下主动脉瓣反流模型,造成少量反流1只,少-中量反流1只,中量反流2只,大量反流1只。其中4只造成瓣叶穿孔,1只造成瓣叶撕裂。结论(:1)超声心动图可以顺利引导导丝穿过主动脉瓣并导致不同程度主动脉瓣损伤,用于小型猪主动脉瓣反流造模方法可靠。(2)超声心动图可明确主动脉瓣损伤部位及损伤程度。
[Abstract]:Objective: to study how to establish aortic regurgitation model of miniature pig by echocardiography. Methods: Chinese miniature pig was selected to measure body weight after general anesthesia and echocardiography was performed to exclude aortic valve disease. The small incision of pig neck was exposed to carotid artery, and the catheter was inserted into the aortic sinus. The catheter was punctured with a hard guide wire to adjust the position of the catheter, resulting in aortic valve injury. Ultrasound was used to evaluate aortic valve injury and reverse flow. The animals were killed, heart was taken and aortic valve damage was observed. Results: a total of 7 Chinese miniature pigs were selected, including 4 males and 3 females, with an average body weight of (24. 7 卤3. 6) kg.. A total of 5 aortic valve regurgitation models were successfully completed, resulting in a small number of regurgitations, a small amount of reflux, a small volume of reflux, a moderate volume of reflux, and a large number of regurgitations. Among them, 4 of them caused perforation of the lobe and 1 caused the tear of the lobe. Conclusion (1) Echocardiography is a reliable method for modeling aortic valve reflux in miniature pigs. (2) Echocardiography can identify the location and degree of aortic valve injury.
【作者单位】： 中国医学科学院北京协和医学院国家心血管病中心阜外医院超声科;首都医科大学附属北京儿童医院心脏超声科;中国医学科学院北京协和医学院国家心血管病中心阜外医院动物实验中心;
【基金】：国家自然基金项目(81271583)
【分类号】：R540.45;R-332;R542.52
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