自旋探针标记的血清白蛋白电子顺磁共振波谱诊断直结肠癌
发布时间：2018-10-08 12:46
【摘要】：近年来,随着人民生活水平的不断提高,饮食习惯和饮食结构的改变以及人口老龄化,我国直结肠癌的发病率和死亡率均呈现上升趋势。直结肠癌发病率和致死率在消化系统恶性肿瘤中仅次于胃癌,食管癌,大多数患者发现时已属于中晚期。 到目前为止,CEA、CA19-9、CA24-2是公认的最可信的与直结肠癌相关的血清肿瘤标志物,但是诊断敏感性和特异性并不高。本实验研究目的是对比直结肠癌病人、健康志愿者血清白蛋白与自旋探针结合能力,并分析自旋探针标记的血清白蛋白电子顺磁共振波谱在直结肠癌诊断中的价值。 随机选取2012年10月至2013年3月收治的141例直结肠癌病人和180例健康志愿者的血清,选用16Doxyl硬脂酸作为自旋探针,通过电子顺磁共振波谱仪获取直结肠癌病人、健康志愿者血清白蛋白与自旋探针结合的波谱图谱。利用从图谱中获得的五个参数(△H1△H2△H3△H4△H5,相对线宽和峰宽),结合统计学中的判别分析方法,建立判别函数。 直结肠癌患者血清白蛋白与自旋探针的结合能力,与健康人的相比,有明显的差异。通过对波谱分析,从波谱中获得5种参数,将这5种参数应用到判别分析的统计学方法中,得到判别分析的函数：F1=338.904△H1+88328.145△H2-103.109△H3+96423.334△H4+265.178△H5-255051.924; F2=328.375AH1+88226.653△H2-116.781△H3+96324.452△H4+259.369△H5-254166.845。诊断特异性和敏感性分别为87.9%和86.1%,证实筛检试验有很大的诊断价值。 直结肠癌病人、健康人血清白蛋白与自旋探针结合能力不同,肿瘤相关的血清白蛋白的变化可以通过自旋标记的电子顺磁共振波谱来评价,自旋探针标记的血清白蛋白电子顺磁共振波谱诊断直结肠癌具有较高的诊断特异性和敏感性,该方法有可能成为临床新的直结肠癌实验室诊断方法。
[Abstract]:In recent years, with the improvement of people's living standard, the change of diet habits and diet structure, and the aging of population, the morbidity and mortality of colon cancer in China are on the rise. The morbidity and mortality of colon cancer are second only to gastric cancer and esophageal carcinoma in digestive system malignant tumor. So far, CA19-9 CA24-2 is recognized as the most reliable serum tumor marker associated with colorectal cancer, but the diagnostic sensitivity and specificity are not high. The aim of this study was to compare the binding ability of serum albumin with spin probe in patients with colon cancer and to analyze the value of electron paramagnetic resonance spectroscopy (EPR) of serum albumin labeled with spin probe in the diagnosis of colon cancer. Serum samples of 141 patients with colorectal cancer and 180 healthy volunteers were randomly selected from October 2012 to March 2013. 16Doxyl stearic acid was used as spin probe to obtain colorectal cancer patients by electron paramagnetic resonance spectroscopy. Spectrogram of the binding of serum albumin with spin probe in healthy volunteers. Using the five parameters (H 1H 2H 3H 4H 5, relative linewidth and peak width) obtained from the map and combining the discriminant analysis method in statistics, the discriminant function was established. The binding ability of serum albumin with spin probe in colon cancer patients was significantly different from that in healthy people. Through the spectral analysis, five parameters were obtained from the spectrum, and the five parameters were applied to the statistical method of discriminant analysis. The function of discriminant analysis was obtained, the function of discriminant analysis was 338.904 H1 88328.145 H2-103.109 H3 96423.334 H4 265.178 H5-255051.924, F2=328.375AH1 88226.653 H2-116.781 H3 96324.452 H4 259.369 H5-254166.845. The diagnostic specificity and sensitivity were 87.9% and 86.1% respectively. In colorectal cancer patients, the binding ability of serum albumin to spin probe is different. The change of serum albumin associated with tumor can be evaluated by spin labeled electron paramagnetic resonance spectroscopy. Electron paramagnetic resonance spectroscopy of serum albumin labeled with spin probe has high diagnostic specificity and sensitivity, and this method may become a new laboratory diagnostic method for colorectal cancer.
【学位授予单位】：北京协和医学院
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R735.34;R445.2
本文编号：2256806
[Abstract]:In recent years, with the improvement of people's living standard, the change of diet habits and diet structure, and the aging of population, the morbidity and mortality of colon cancer in China are on the rise. The morbidity and mortality of colon cancer are second only to gastric cancer and esophageal carcinoma in digestive system malignant tumor. So far, CA19-9 CA24-2 is recognized as the most reliable serum tumor marker associated with colorectal cancer, but the diagnostic sensitivity and specificity are not high. The aim of this study was to compare the binding ability of serum albumin with spin probe in patients with colon cancer and to analyze the value of electron paramagnetic resonance spectroscopy (EPR) of serum albumin labeled with spin probe in the diagnosis of colon cancer. Serum samples of 141 patients with colorectal cancer and 180 healthy volunteers were randomly selected from October 2012 to March 2013. 16Doxyl stearic acid was used as spin probe to obtain colorectal cancer patients by electron paramagnetic resonance spectroscopy. Spectrogram of the binding of serum albumin with spin probe in healthy volunteers. Using the five parameters (H 1H 2H 3H 4H 5, relative linewidth and peak width) obtained from the map and combining the discriminant analysis method in statistics, the discriminant function was established. The binding ability of serum albumin with spin probe in colon cancer patients was significantly different from that in healthy people. Through the spectral analysis, five parameters were obtained from the spectrum, and the five parameters were applied to the statistical method of discriminant analysis. The function of discriminant analysis was obtained, the function of discriminant analysis was 338.904 H1 88328.145 H2-103.109 H3 96423.334 H4 265.178 H5-255051.924, F2=328.375AH1 88226.653 H2-116.781 H3 96324.452 H4 259.369 H5-254166.845. The diagnostic specificity and sensitivity were 87.9% and 86.1% respectively. In colorectal cancer patients, the binding ability of serum albumin to spin probe is different. The change of serum albumin associated with tumor can be evaluated by spin labeled electron paramagnetic resonance spectroscopy. Electron paramagnetic resonance spectroscopy of serum albumin labeled with spin probe has high diagnostic specificity and sensitivity, and this method may become a new laboratory diagnostic method for colorectal cancer.
【学位授予单位】：北京协和医学院
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R735.34;R445.2
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