自制纳米级E-选择素靶向超声微泡对缺血心肌分子成像

发布时间：2018-11-06 11:27

【摘要】：目的采用自制的携E-选择素抗体的纳米级靶向超声造影剂对大鼠缺血再灌注损伤后的缺血心肌进行超声分子成像,评估其检测缺血心肌的可行性。方法采用生物素-链亲和素方法制备纳米级靶向微泡,应用荧光显微镜及流式细胞仪检测微泡形态及抗体连接效率;将26只SD大鼠随机分为E-选择素靶向微泡组(MBE组,n=10)、IGg抗体微泡组(MBIGg组,n=10)、普通微泡组(MBC组,n=6),制备心肌缺血再灌注损伤模型,将冠状动脉左前降支阻断15min后恢复灌注,于再灌注后6h经尾静脉分别注射3种微泡行CEUS。后行病理学检查。结果 MBE组缺血区域声学强度(VI)显著高于非缺血区(P0.05),也显著高于MBC组、MBIGg组缺血区域的VI(P0.05);MBC组、MBIGg组缺血区域与非缺血区的VI差异无统计学意义(P0.05)。结论纳米级E-选择素靶向超声微泡可以早期检测到缺血心肌,有望实现缺血心肌的"记忆"成像。
[Abstract]:Aim to evaluate the feasibility of ultrasonic molecular imaging of ischemic myocardium after ischemia-reperfusion injury in rats by using a self-made nano-targeted ultrasound contrast medium carrying E- selectin antibody. Methods Nano-targeted microbubbles were prepared by biotin-chain avidin method. The morphology of microbubbles and antibody binding efficiency were detected by fluorescence microscope and flow cytometry. Twenty-six SD rats were randomly divided into E- selectin targeted microbubble group (MBE group), nnn10), IGg antibody microbubble group (MBIGg group) and ordinary microbubble group (MBC group, nong6) to prepare myocardial ischemia-reperfusion injury model. The left anterior descending coronary artery was blocked and the perfusion was restored after occlusion of the left anterior descending branch of the coronary artery. CEUS. was performed by injecting three kinds of microbubbles through the caudal vein 6 hours after reperfusion. Pathological examination was performed afterwards. Results the acoustic intensity of (VI) in ischemic area of MBE group was significantly higher than that in non-ischemic area (P0.05) and significantly higher than that in MBC group (P0.05). VI in ischemic region of MBIGg group was significantly higher than that in MBIGg group (P0.05). There was no significant difference in VI between ischemic area and non ischemic area in MBC group and MBIGg group (P0.05). Conclusion Nano-scale E-selectin-targeted ultrasound microbubbles can detect ischemic myocardium early and may be able to realize memory imaging of ischemic myocardium.
【作者单位】：哈尔滨医科大学附属第二医院超声医学科;
【基金】：国家自然科学基金(81371569) 黑龙江省教育厅海外学人科研资助项目(1252HQ015) 哈尔滨市科技创新人才研究专项基金项目(2012RFLXS018)
【分类号】：R445.1

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