纳米级阳离子脂质微泡的制备与评价

发布时间：2019-01-10 16:01

【摘要】：目的制备纳米级阳离子脂质微泡,并评价其理化特性及体内显影效果。方法选用氨基甲酰基胆固醇(DC-chol)作为微泡阳离子成分,通过薄膜水化法及机械振荡法制备纳米级阳离子脂质微泡,以SonoVue为对照对自制微泡形态分布、平均粒径、表面电荷、浓度进行评测,并观察其在动物体内显影效果。结果光学显微镜及电子显微镜下微泡分散性好、形态规则呈球形;马尔文电位仪测得平均粒径(442.6±58.75)nm,平均表面电位为+(41.8±10.2)mV,连续观察1周内稳定性好;动物体内显影效果强度略低于SonoVue,但增强持续时间较长。结论自制纳米级阳离子脂质微泡具有粒径更小、带正电荷可携载基因及体内显影效果良好的特点,具有作为基因载体进行体内转染的巨大潜力。
[Abstract]:Objective to prepare nano-sized cationic lipid microbubbles and evaluate their physicochemical properties and in vivo development effect. Methods using carbamate cholesterol (DC-chol) as the cationic component of microbubbles, nano-sized cationic lipid microbubbles were prepared by membrane hydration and mechanical oscillation. The morphology distribution and average particle size of self-made cationic lipids were compared with those of SonoVue. The surface charge and concentration were evaluated and the development effect in animals was observed. Results the dispersion of microbubbles was good under optical microscope and electron microscope, and the morphology of microbubbles was regular and spherical. Ma Erwen potentiometer measured the mean particle size (442.6 卤58.75) nm, mean surface potential was (41.8 卤10. 2) mV, continuously observed within one week, the stability was good, the development effect intensity in animal was slightly lower than SonoVue, but the enhancement duration was longer. Conclusion the nanosized cationic lipid microbubbles have the advantages of smaller particle size, positive charge carrying genes and good development effect in vivo, and have great potential as gene carriers for in vivo transfection.
【作者单位】：宁夏医科大学;宁夏医科大学总医院超声科;
【基金】：国家自然科学基金(No.81360225)
【分类号】：R445.1

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