慢性阻塞性肺疾病患者外周血单个核细胞NOD2 mRNA表达及白细胞介素-6的水平及意义

发布时间：2018-02-22 18:49

本文关键词： 慢性阻塞性肺疾病 NOD 白细胞介素　出处：《中国老年学杂志》2017年14期 　论文类型：期刊论文

【摘要】：目的探讨外周血单个核细胞NOD2 mRNA和血清白细胞介素(IL)-6在慢性阻塞性肺疾病(COPD)发病机制中的作用及其与COPD患者严重程度的关系。方法对经过门诊及住院达到稳定期的COPD患者进行综合评估,分为A、B、C、D四组,每组30例,同期门诊健康体检者30例作为健康对照组,采用PCR和ELISA法分别检测COPD各组及健康对照组外周血单个核细胞核内NOD2 mRNA的表达及血清中IL-6的含量。结果 COPD稳定期患者外周血单个核细胞核内NOD2 mRNA及血清IL-6的表达均明显高于健康对照组(P0.05)。COPD患者A、B、C、D各组中NOD2 mRNA和IL-6的表达明显高于健康对照组(P0.05)。COPD患者D组NOD2 mRNA和IL-6的表达明显高于C组(P0.05),C组和D组的NOD2 mRNA和IL-6的表达均明显高于A组和B组(P0.05),A组和B组之间无统计学差异(P0.05)。COPD患者NOD2 mRNA表达和IL-6的含量呈正相关(r=0.81,P0.05)。结论 NOD2和IL-6都参与了COPD气道炎症的发生发展,其水平与COPD患者严重程度呈正相关。
[Abstract]:Objective to investigate the role of peripheral blood mononuclear cells (NOD2 mRNA) and interleukin-6 (IL-6) in the pathogenesis of chronic obstructive pulmonary disease (COPD) and its relationship with the severity of COPD. Comprehensive assessment of COPD patients, The patients were divided into four groups, 30 cases in each group, and 30 cases as healthy control group in the same period. PCR and ELISA were used to detect the expression of NOD2 mRNA in peripheral blood mononuclear nucleus and the content of IL-6 in serum in COPD group and healthy control group respectively. Results NOD2 mRNA and IL-6 in peripheral blood mononuclear nucleus of COPD stable phase patients were detected. The expression of NOD2 mRNA and IL-6 in group D was significantly higher than that in group D (P 0.05). The expression of NOD2 mRNA and IL-6 in group D was significantly higher than that in group C and group D. The expression of NOD2 mRNA and IL-6 in group C and group D were significantly higher than those in group C and group D. There was no significant difference between group A and group B in the expression of NOD2 mRNA and the content of IL-6. Conclusion both NOD2 and IL-6 are involved in the occurrence and development of COPD airway inflammation. Its level was positively correlated with the severity of COPD.
【作者单位】：川北医学院附属医院呼吸内科;
【基金】：四川省教育厅科研基金项目(No.13ZA0216)
【分类号】：R563.9

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