转化生长因子-β对肥胖哮喘小鼠气道重塑的影响及吡非尼酮的干预作用

发布时间：2018-02-23 12:53

本文关键词： 哮喘肥胖重塑吡非尼酮转化生长因子β　出处：《中国呼吸与危重监护杂志》2017年03期 　论文类型：期刊论文

【摘要】：目的探讨转化生长因子-β(TGF-β)对肥胖哮喘小鼠气道重塑的影响及吡非尼酮干预的作用。方法 75只C57BL/6J小鼠随机分为对照组(A组)、肥胖组(B组)、肥胖哮喘组(C组)、布地奈德治疗组(D组)、吡非尼酮治疗组(E组)5组,每组15只。肥胖小鼠以高脂饮食诱导肥胖模型,C、D、E组采用卵清蛋白致敏激发建立哮喘模型,对照组以普通饲料饲养,生理盐水致敏激发。D组以布地奈德悬液(0.5 mg/ml)雾化,E组以吡非尼酮(300 mg/kg)饮水干预,余组正常饮水。4周后收集支气管肺泡灌洗液(BALF)进行细胞计数及分类。肺组织切片观察小鼠气道炎症及重塑情况,测定气管壁总面积(WAt)、气管平滑肌面积(WAm)和管腔基底膜周长(Pbm)。采用酶联免疫吸附实验、蛋白质印迹法检测TGF-β水平。结果 C组小鼠BALF中白细胞总数、嗜酸性粒细胞百分比、TGF-β水平、气管壁厚度(WAt/Pbm)和平滑肌厚度(WAm/Pbm)均高于其他四组;E组BALF中嗜酸性粒细胞百分比、WAt/Pbm较D组减少(P均0.05);TGF-β水平按照C、D、E、B、A组依次递减(P均0.05),且与BALF中嗜酸性粒细胞百分比呈正相关(r=0.79,P均0.01)。结论 TGF-β在肥胖哮喘小鼠气道中高表达,与气道重塑有密切关系。吡非尼酮能有效抑制TGF-β的表达,从而改善肥胖哮喘气道重塑。
[Abstract]:Objective to investigate the effect of transforming growth factor- 尾 (TGF- 尾) on airway remodeling in obese asthmatic mice and the effect of pifenidone on airway remodeling. Methods Seventy-five C57BL / 6J mice were randomly divided into three groups: control group A, obese group B, obese asthma group C, Bud. Group D was treated with acetonide and group E with pyfenidone (group 5). There were 15 mice in each group. The obese mice were induced by high-fat diet. The asthmatic model was induced by ovalbumin (ovalbumin) sensitization in group E, and the control group was fed with common diet. Group D was treated with budesonide suspension 0.5 mg / ml) nebulized E group treated with pifenidone 300 mg / kg drinking water. BALF was collected from bronchoalveolar lavage fluid (BALF) for cell count and classification after 4 weeks of normal drinking water. Lung tissue sections were used to observe airway inflammation and remodeling in mice. The total area of trachea wall, the area of smooth muscle of trachea (Wam) and the circumference of basal membrane of trachea were measured. The levels of TGF- 尾 in BALF of group C were measured by Elisa and Western blotting. Results the total number of leukocytes and the percentage of eosinophils in group C were determined by TGF- 尾. The percentage of eosinophilic granulocytes in BALF of group E was significantly higher than that of group D (P < 0.05), and the percentage of eosinophil in group E was positively correlated with the percentage of eosinophil in BALF. Conclusion TGF- 尾 is highly expressed in the airway of obese asthmatic mice. Pifenidone can effectively inhibit the expression of TGF- 尾 and improve airway remodeling in obese asthma.
【作者单位】：青岛大学医学院附属青岛市市立医院东院呼吸科;青岛市市立医院(集团)东院;
【基金】：国家自然科学基金(81400024) 山东省自然科学基金(ZR2014HM105)
【分类号】：R562.25

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