HIF-1α在间歇低氧合并肺气肿大鼠肝细胞凋亡中的机制研究

发布时间：2018-04-03 17:57

本文选题：缺氧诱导因子　切入点：缺氧　出处：《天津医药》2014年02期

【摘要】：目的探讨间歇低氧(IH)合并肺气肿对大鼠肝脏低氧诱导因子-1α(HIF-1α)、Bax、Bcl-2表达的影响及HIF-1α在肝细胞凋亡中的作用机制。方法将60只大鼠按随机数字表法均分为4组:正常对照组,正常饲养;IH组,每天9:00—17:00于低氧舱内交替通入30 s氮气和90 s空气,8 h/d;肺气肿组,每天8:00和18:00于熏箱内熏烟各30 min;IH合并肺气肿组,上述IH组和肺气肿组方法的联合。暴露14周后,处死大鼠,采用荧光实时定量(qRT)-PCR法测定肝组织中HIF-1α、Bax、Bcl-2表达水平。结果 IH合并肺气肿组HIF-1αmRNA、Bax mRNA、Bax/Bcl-2水平较正常对照组、IH组、肺气肿组升高(均P0.05),Bcl-2 mRNA水平较正常对照组、肺气肿组降低(均P0.05),较IH组无显著差异(P0.05);HIF-1α与Bax、Bax/Bcl-2呈正相关(r分别为0.732、0.699),与Bcl-2呈负相关(r=-0.705)。结论 IH合并肺气肿可诱导肝细胞HIF-1αmRNA、Bax mRNA、Bax/Bcl-2表达上调,HIF-1α与Bax、Bcl-2相关,HIF-1α可能通过调控凋亡基因Bax、Bcl-2的表达来参与肝细胞的凋亡。
[Abstract]:Objective to investigate the effect of intermittent hypoxia (IH) combined with emphysema on the expression of Bcl-2 in rat liver hypoxia inducible factor-1 伪 (HIF-1 伪) and the role of HIF-1 伪 in hepatocyte apoptosis.Every day at 8:00 and 18:00 in the fumigation box 30 min each of IH combined with emphysema group, the combination of the above mentioned IH group and emphysema group.After 14 weeks of exposure, the rats were killed and the expression of HIF-1 伪 Baxon Bcl-2 in liver tissue was measured by real-time fluorescence quantitative RT-PCR.Results HIF-1 伪 mRNA-Bax mRNA-Bax mRNA-Bax mRNA-Bax-Bcl-2 in IH with emphysema group was higher than that in normal control group.Conclusion IH combined with emphysema can induce the expression of HIF-1 伪 mRNA-Bax mRNA-Bax mRNA-Bax / Bcl-2 in hepatocytes and up-regulate the expression of Bax mRNA-Bax mRNA-Bax mRNA-Bax / Bcl-2 in hepatocytes.
【作者单位】：天津医科大学;天津市海河医院;天津医科大学总医院;
【基金】：国家自然科学基金资助项目(项目编号:81270144) 天津市自然科学基金资助项目(项目编号:13JCYBJC22400,13JCYBJC40000)
【分类号】：R563.3

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