瑞芬太尼对兔油酸性急性肺损伤的影响

发布时间：2018-06-06 08:33

本文选题：哌啶类 + 油酸　；参考：《东南大学学报(医学版)》2015年01期

【摘要】：目的:探讨瑞芬太尼对兔油酸性急性肺损伤(ALI)的影响。方法:选取健康成年雄性新西兰大白兔18只,由东南大学医学院实验动物中心提供,体质量2.5~3.5 kg,随机分为3组(n=6),即对照组、油酸组和瑞芬太尼组。对照组经30 min静脉输注生理盐水10 ml;油酸组经30 min静脉注射油酸0.1 ml·kg-1;瑞芬太尼组静脉输注瑞芬太尼0.2μg·kg-1·min-1至处死,输注15 min时开始给予油酸,方法同油酸组。各组大白兔分别在输入瑞芬太尼或生理盐水(以下简称给药)前和给药后6 h记录平均动脉血氧分压(Pa O2),及ELISA法检测血浆细胞黏附分子1(ICAM-1)、TNF-α及IL-8浓度。给药6 h后处死动物并迅速剖胸取肺脏,肉眼观察肺组织病理改变,留取肺组织标本行电镜观察肺组织超微结构变化和测定湿干质量比。结果:瑞芬太尼组肺组织损伤程度逐渐减轻,明显轻于油酸组,瑞芬太尼能显著降低油酸性ALI兔血浆中ICAM-1、TNF-α及IL-8水平,改善ALI兔肺水肿,从而使PCO2和Pa O2均明显改善。结论:瑞芬太尼能有效地抑制血管内皮细胞及肺泡上皮细胞的损伤和炎症细胞浸润,阻滞ALI时失控的炎症反应,其机制可能与抑制ICAM-1、TNF-α及IL-8的表达有关。
[Abstract]:Objective: To investigate the effect of remifentanil on acute lung injury (ALI) in rabbits. Methods: 18 healthy adult male New Zealand white rabbits were selected from the experimental animal center of the Medical College of Southeast University. The body mass 2.5~3.5 kg was randomly divided into 3 groups (n=6), that is, the control group, the oleic acid group and remifentanil group. The control group was injected with 30 min intravenous infusion of saline. 10 ml; oleic acid group was injected with oleic acid 0.1 ml. Kg-1 via 30 min; remifentanil infusion was injected with remifentanil 0.2 mu g. Min-1 to death. When infusion 15 min, oleic acid was given to oleic acid group. The average arterial oxygen fraction was recorded before and after input of remifentanil or physiological saline (below a simple dose) and 6 h after administration. Pressure (Pa O2), and ELISA method were used to detect plasma cell adhesion molecule 1 (ICAM-1), TNF- alpha and IL-8 concentration. After 6 h, the animals were killed and the lungs were quickly removed. The pathological changes of lung tissue were observed by the naked eye. The ultrastructural changes of lung tissue and the ratio of wet dry mass were observed by the microscopic examination of lung tissue. Results: the degree of lung tissue injury in remifentanil group was the degree of lung tissue injury. Gradually reducing, obviously lighter than oleic acid group, remifentanil can significantly reduce the levels of ICAM-1, TNF- A and IL-8 in oleic acid ALI rabbit plasma, improve the pulmonary edema in ALI rabbit, thus improve both PCO2 and Pa O2. Conclusion: remifentanil can effectively inhibit the damage of vascular endothelial cells and alveolar epithelial cells and inflammatory cell infiltration, and prevent ALI out of control when ALI. The mechanism of inflammation may be related to the inhibition of the expression of ICAM-1, TNF- and IL-8.
【作者单位】：南京市胸科医院重症医学科;东南大学附属中大医院呼吸科;
【基金】：南京市医学科技发展课题(YKK12130)
【分类号】：R563.8

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