人类白细胞抗原DQA1基因多态性与尘肺病发病相关性分析

发布时间：2018-08-27 12:07

【摘要】：目的探讨人类白细胞抗原(HLA)-DQA1位点基因多态性与尘肺病发病的相关性。方法采用1∶1配比的病例-对照研究方法,选取130例已确诊为尘肺病的患者作为尘肺组,另选130例与尘肺组年龄和累计接尘工龄相近、同性别、同民族、同一工作地点的健康接尘工人作为对照组。用多重聚合酶链反应-连接酶检测反应分型法,检测2组研究对象HLA-DQA1共6个位点的等位基因,并分析其与尘肺病的相关性。结果尘肺组HLA-DQA1*0201和HLA-DQA1*0601基因频率均高于对照组(14.62%vs 8.85%,12.69%vs 7.31%),而HLA-DQA1*0101基因频率低于对照组(34.23%vs 43.08%),差异均有统计学意义(P0.05)。Logistic回归分析显示,HLA-DQA1*0101、HLA-DQA1*0201和HLA-DQA1*0601等位基因频率与尘肺病有关联(P0.05),优势比分别为0.688、1.764和1.844,95%可信区间分别为(0.482~0.981)、(1.018~3.055)和(1.019~3.336)。结论 HLA-DQA1*0201和HLA-DQA1*0601基因可能是尘肺病的易感基因,HLA-DQA1*0101基因可能是尘肺病的保护基因。
[Abstract]:Objective to investigate the relationship between (HLA) -DQA1 locus polymorphism of human leukocyte antigen and pneumoconiosis. Methods 130 patients with pneumoconiosis were selected as pneumoconiosis group by using the 1:1 matched case-control study, and 130 patients with pneumoconiosis were selected as the same age and cumulative exposure age, same sex and same nationality. Healthy workers exposed to dust in the same workplace served as the control group. Multiplex polymerase chain reaction-ligase assay was used to detect the alleles of 6 HLA-DQA1 loci in two groups of subjects, and the correlation between the alleles and pneumoconiosis was analyzed. Results the frequencies of HLA-DQA1*0201 and HLA-DQA1*0601 genes in pneumoconiosis group were higher than those in control group (14.62%vs 8.85: 12.69 vs 7.31%), while the frequency of HLA-DQA1*0101 gene was lower than that in control group (34.23%vs 43.08%). The difference was statistically significant (P0.05). Logistic regression analysis showed that the allele frequencies of HLA-DQA1-0101 HLA-DQA1-0201 and HLA-DQA1*0601 were associated with pneumoconiosis (P0.05). The confidence intervals of 0.688), (1.764 and 1.844V 95% were (0.482n) 0.981), (1.018 / 3.055 and (1.019 / 3.336), respectively. Conclusion HLA-DQA1*0201 and HLA-DQA1*0601 genes may be the susceptible genes of pneumoconiosis, and HLA-DQA1O101 gene may be a protective gene for pneumoconiosis.
【作者单位】：山东省医学科学院山东省肿瘤医院;济南大学山东省医学科学院医学与生命科学学院;淄博市职业病防治院;
【分类号】：R135.2

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