反复接种BCG对慢性哮喘小鼠IL-33表达的影响
发布时间：2018-12-29 10:39
【摘要】：目的：研究在慢性哮喘模型中,BCG对哮喘的保护是否通过IL-33介导,为研发哮喘的新治疗方法提供理论基础。 方法：通过建立慢性哮喘小鼠模型,观察三组(正常小鼠、慢性哮喘模型小鼠、BCG干预组)肺组织IL-33的mRNA表达,支气管肺泡灌洗液及小鼠血清中IL-33表达。 结果：在慢性哮喘模型中,小鼠支气管肺泡灌洗液(BALF)嗜酸性细胞计数在模型组(8.429±5.711*104/ml个)显著高于对照组(0.286±0.488*104/ml个),说明慢性哮喘模型建模成功(n=7,P0.01)。实验组(3.714±2.2887*104/ml个)显著低于建模组(n=7,P0.05)。在慢性哮喘模型中,IL-33在模型组小鼠肺组织中的表达高于正常对照小鼠组4.154±1.020倍(n=6,P0.01)。实验组小鼠肺组织中IL-33mRNA为对照组的2.791±1.230倍(n=6,P0.05) 结论：在慢性哮喘模型小鼠中,BCG干预可以降低肺部嗜酸性粒细胞浸润。慢性哮喘模型小鼠肺组织IL-33mRNA显著高于生理盐水组,但BCG干预组的IL-33则显著降低。提示BCG对哮喘的保护机制可能通过IL-33介导。
[Abstract]:Aim: to study whether the protection of BCG against asthma in chronic asthma model is mediated by IL-33, and to provide a theoretical basis for the development of a new treatment for asthma. Methods: the mRNA expression of IL-33, the expression of IL-33 in bronchoalveolar lavage fluid (BALF) and serum of three groups (normal mice, chronic asthmatic mice, BCG intervention group) were observed. Results: in the model of chronic asthma, the eosinophil count of (BALF) in the bronchoalveolar lavage fluid was significantly higher in the model group (8.429 卤5.711*104/ml) than in the control group (0.286 卤0.488*104/ml). The results showed that the model of chronic asthma was successfully established (n = 7, P 0.01). The experimental group (3.714 卤2.2887*104/ml) was significantly lower than that of the model group (P 0.05). In chronic asthma model, the expression of IL-33 in lung tissue of model group was 4.154 卤1.020 times higher than that of normal control group. IL-33mRNA in the lung tissue of the experimental group was 2.791 卤1.230 times of that of the control group (P 0.05). Conclusion: in the model mice with chronic asthma, BCG intervention can reduce the infiltration of eosinophils in the lung. IL-33mRNA in lung tissue of chronic asthmatic mice was significantly higher than that of saline group, but IL-33 in BCG intervention group was significantly lower. The results suggest that the protective mechanism of BCG against asthma may be mediated by IL-33.
【学位授予单位】：浙江大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R562.25
本文编号：2394733
[Abstract]:Aim: to study whether the protection of BCG against asthma in chronic asthma model is mediated by IL-33, and to provide a theoretical basis for the development of a new treatment for asthma. Methods: the mRNA expression of IL-33, the expression of IL-33 in bronchoalveolar lavage fluid (BALF) and serum of three groups (normal mice, chronic asthmatic mice, BCG intervention group) were observed. Results: in the model of chronic asthma, the eosinophil count of (BALF) in the bronchoalveolar lavage fluid was significantly higher in the model group (8.429 卤5.711*104/ml) than in the control group (0.286 卤0.488*104/ml). The results showed that the model of chronic asthma was successfully established (n = 7, P 0.01). The experimental group (3.714 卤2.2887*104/ml) was significantly lower than that of the model group (P 0.05). In chronic asthma model, the expression of IL-33 in lung tissue of model group was 4.154 卤1.020 times higher than that of normal control group. IL-33mRNA in the lung tissue of the experimental group was 2.791 卤1.230 times of that of the control group (P 0.05). Conclusion: in the model mice with chronic asthma, BCG intervention can reduce the infiltration of eosinophils in the lung. IL-33mRNA in lung tissue of chronic asthmatic mice was significantly higher than that of saline group, but IL-33 in BCG intervention group was significantly lower. The results suggest that the protective mechanism of BCG against asthma may be mediated by IL-33.
【学位授予单位】：浙江大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R562.25
【共引文献】
相关期刊论文 前2条
1 麻晓燕;罗雅玲;赖文岩;邵金莲;蓝海兵;梁振宇;;支气管哮喘患者外周血、痰液中IL-33的含量及其相关性分析[J];第三军医大学学报;2011年14期
2 年四季;张金平;高燕;杨燕;向丽;袁青;;重组IL-33融合表达载体的构建、蛋白表达及纯化[J];泸州医学院学报;2011年05期
，本文编号：2394733
本文链接：https://www.wllwen.com/yixuelunwen/huxijib/2394733.html
最近更新
教材专著
