老龄贵州小型猪胃黏膜5-HT-IR及SS-IR细胞的初步观察

发布时间：2017-12-27 09:02

本文关键词：老龄贵州小型猪胃黏膜5-HT-IR及SS-IR细胞的初步观察　出处：《西北农林科技大学学报(自然科学版)》2013年08期 　论文类型：期刊论文

【摘要】：【目的】观察、比较分析老龄贵州小型猪胃内分泌细胞的形态、数量和分布。【方法】采集老龄和青年贵州小型猪的胃,取胃的不同部位(贲门、胃体、胃窦),用免疫组织化学染色法分别对老龄和青年贵州小型猪胃黏膜中的5-羟色胺(5-HT)、生长抑素(SS)细胞染色,在400倍视野中观察其形态及分布,并进行计数,比较老龄与青年贵州小型猪胃黏膜的差异。【结果】老龄和青年贵州小型猪胃黏膜中的5-HT-IR、SS-IR阳性细胞呈锥形、圆形、卵圆形等多种形态;分布不均匀,分别主要分布于胃腺的体部、底部或黏膜表面上皮细胞之间。老龄贵州小型猪胃窦5-HT-IR阳性细胞数较贲门极显著增加(P0.01),胃体较贲门显著增加(P0.05);胃窦SS-IR阳性细胞数较贲门极显著增加(P0.01),而胃体与贲门差异不显著。老龄贵州小型猪5-HT-IR、SS-IR阳性细胞数较青年贵州小型猪明显减少,且差异达极显著水平(P0.01)。【结论】老龄贵州小型猪胃黏膜5-HT-IR、SS-IR阳性细胞的形态、分布规律与青年贵州小型猪相似,但分泌5-HT、SS的细胞数量减少,提示老龄贵州小型猪的胃黏膜内分泌功能减退。
[Abstract]:[Objective] to observe and compare the morphology, quantity and distribution of gastric endocrine cells in aged Guizhou miniature pigs. [method] collected in aged and young Guizhou mini pigs in different parts of the stomach, the stomach (cardia, gastric body and antrum), by immunohistochemical staining respectively in aged and young Guizhou mini pigs in gastric mucosa of 5- hydroxytryptamine (5-HT), somatostatin (SS) to observe the morphology and cell staining. The distribution of 400 times in the field of vision, and count, the difference in age and youth of Guizhou miniature pig gastric mucosa. [results] the positive cells of 5-HT-IR and SS-IR in gastric mucosa of old and young Guizhou miniature pigs were cone-shaped, round and oval, and their distribution was uneven. They were mainly distributed in the body, bottom or mucosal surface epithelial cells of gastric glands. The number of 5-HT-IR positive cells in gastric antrum increased significantly (P0.01). The gastric body increased significantly compared with the cardia (P0.05). The number of positive cells in gastric antrum SS-IR was significantly higher than that in gastric cardia (P0.01), but the difference between gastric body and gastric cardia was not significant (P0.01). The number of 5-HT-IR and SS-IR positive cells in aged Guizhou miniature pigs decreased significantly than that of young Guizhou miniature pigs, and the difference was very significant (P0.01). [Conclusion] the morphology and distribution of 5-HT-IR and SS-IR positive cells in gastric mucosa of old Guizhou miniature pigs are similar to those of young Guizhou miniature pigs, but the number of cells secreting 5-HT and SS decreases, suggesting that the endocrine function of gastric mucosa of elderly Guizhou miniature pigs is reduced.
【作者单位】：贵阳中医学院实验动物研究所;贵阳中医学院组织学与胚胎学教研室;贵阳中医学院基础医学院;
【基金】：“十五”国家科技攻关计划重点项目(2004BA717B-01-03) 贵州省科技计划项目(黔科合中药字[P2012]LKZ7001号)
【分类号】：R-332
【正文快照】： 小型猪作为实验动物的优越性已被越来越多的人所认知,但由于其基础及应用研究背景资料不足,小型猪还未能取代犬和非人灵长类实验动物,在生物医学研究中充分体现其重要价值。消化道不仅是消化器官,也是体内最大、最复杂的内分泌器官,其内分泌细胞种类繁多,功能各异。消化道内

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