原位获取内皮细胞构建兔颈静脉去内皮细胞和血栓形成模型

发布时间：2017-12-27 09:18

本文关键词：原位获取内皮细胞构建兔颈静脉去内皮细胞和血栓形成模型　出处：《上海医学》2013年12期 　论文类型：期刊论文

【摘要】：目的采用Ⅰ型胶原酶原位灌注消化法原位获取兔颈静脉的内皮细胞,构建兔颈静脉的内皮剥脱和血栓形成模型。方法新西兰大白兔12只,雌雄不拘,随机分入抗凝组和对照组。①分离兔右侧颈静脉,保留对侧颈静脉,采用改进的Ⅰ型胶原酶原位灌注消化法分离兔颈静脉内皮细胞,体外培养,并进行血管内皮细胞鉴定。②抗凝组兔腹部皮下注射低分子肝素钠,对照组不给予抗凝血药物,7d后处死动物,检查右颈静脉的通畅情况。结果抗凝组中,1只兔在术后第3天出现口腔和鼻腔出血,粪便带血,手术切口出血而死亡,其右侧颈静脉通畅,颈部切口内有明显血肿。存活5只:颈静脉通畅2只;颈静脉内少量血栓形成1只,管腔轻度狭窄,管腔残余内径为对侧颈静脉的80%;颈静脉内中等程度血栓形成1只,管腔中度狭窄,管腔残余内径为对侧颈静脉的50%;兔颈静脉内血栓形成、管腔完全堵塞1只。对照组中,术后第2天开始出现球结膜水肿1只,肢体和躯干运动减少,术后第4天死亡,其右侧颈静脉内血栓形成,管腔完全堵塞,血栓向下延伸到上腔静脉,造成上腔静脉管腔堵塞;右侧颈静脉内血栓形成、管腔完全堵塞5只,均存活。结论Ⅰ型胶原酶原位灌注消化法构建兔颈静脉去内皮和血栓形成模型具有一定的可行性,可为研究颈静脉血管去内皮后如何预防血栓形成的药物学方法和血栓形成以后的治疗手段提供实验对象。
[Abstract]:Objective to obtain endothelial cells from rabbit jugular vein in situ by type I collagenase in situ perfusion method, and to construct the model of endothelial denudation and thrombosis in the jugular vein of rabbits. Methods 12 New Zealand white rabbits were randomly divided into the anticoagulant group and the control group. First, the right jugular vein was isolated from the right side of the jugular vein, and the contralateral jugular vein was reserved. The rabbit carotid vein endothelial cells were isolated by improved type I collagenase perfusion in situ, and cultured in vitro, and the endothelial cells were identified. The low molecular weight heparin sodium was injected subcutaneously into the abdomen of the anticoagulant group, the control group did not give anticoagulant drugs, and the animals were killed after 7d, and the patency of the right jugular vein was checked. Results in the anticoagulant group, 1 rabbits developed oral and nasal bleeding on the third day after operation. The blood was excreted from the stool and died after operation incision bleeding. The right jugular vein was unobstructed and there was obvious hematoma in the neck incision. Only 5 survived: jugular venous patency 2; jugular vein and small thrombosis in 1, mild stenosis, residual lumen diameter of jugular vein 80%; jugular vein thrombosis in moderate 1, moderate stenosis lumen diameter, lumen residual contralateral jugular vein 50%; rabbit jugular vein thrombosis the formation, lumen completely blocked 1. In the control group, second days postoperatively chemosis 1, limbs and trunk movements reduced, died fourth days after the operation, the right jugular vein thrombosis, lumen completely blocked, thrombus extending down into the superior vena cava, resulting in superior vena cava lumen; right jugular vein thrombosis, lumen completely block 5, survived. Conclusion typeI in situ collagenase perfusion digestion method to construct rabbit jugular vein endothelial and thrombosis model has certain feasibility to study endothelial jugular vein after how to prevent thrombosis after pharmacological method and formation treatment for experiment.
【作者单位】：上海交通大学医学院附属上海儿童医学中心心胸外科;
【分类号】：R-332
【正文快照】： 血管内皮细胞是覆盖在血管腔内面的一层扁平细胞,具有抗血栓、维持血管内血液流动性的作用[1]。临床工作中,一些操作会引起内皮细胞的损伤和脱落,造成血管内皮层完整性的破坏,从而引起血栓形成。特别是经颈内静脉置入中心静脉导管后出现的颈内静脉或上腔静脉血栓形成,给患者带

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