固本防哮饮对哮喘缓解期模型小鼠IL-17A、IL-10和CXCL10的影响

发布时间：2017-12-28 07:47

本文关键词：固本防哮饮对哮喘缓解期模型小鼠IL-17A、IL-10和CXCL10的影响　出处：《南京中医药大学》2017年硕士论文　论文类型：学位论文

【摘要】：目的:哮喘是一种慢性炎症性呼吸道疾病,严重威胁人类的健康,其发病率和病死率呈逐年增长的趋向。中药复方固本防哮饮是全国著名中医儿科专家江育仁教授经验方,临床运用多年,疗效确切,其可增强患儿体质,预防哮喘发作,提高患儿的生存质量与哮喘长期缓解率。在生物医学领域,已经明确了白细胞介素-17A(IL-17A)、白细胞介素-10(IL-10)及CXC趋化因子配体10(CXCL10)与哮喘发生的密切相关性,固本防哮饮的治疗机制可能与此有关。本研究采用哮喘缓解期动物模型,研究中药复方固本防哮饮对哮喘缓解期小鼠IL-17A、IL-10及CXCL10表达的影响,观察其对哮喘缓解期模型小鼠气道炎症的影响及防治哮喘的机制。方法:采用卵蛋白(OVA)致敏和呼吸道合胞病毒(RSV)滴鼻诱导激发BALB/c雌性小鼠,建立哮喘缓解期动物模型,随机分为正常组,模型组,固本防哮饮低、中、高剂量组,孟鲁司特钠组及地塞米松组。观察各组小鼠的一般行为活动,用ELISA法检测肺组织中IL-17A、IL-10及CXCL10蛋白的表达水平,Real-timePCR法检测肺组织中IL-17A、IL-10、CXCL10mRNA的表达水平。最后采用统计学方法进行分析处理。结果:模型组小鼠肺组织中IL-17A、CXCL10表达均显著高于正常组(P0.01),而IL-10表达显著低于正常组(P0.01);固本防哮饮低、中、高剂量组,孟鲁司特钠组及地塞米松组小鼠肺组织中IL-17A、CXCL10表达均低于模型组(P0.05),而IL-10表达高于模型组(P0.05)。相关性分析显示,ELISA检测法中模型组小鼠IL-10与IL-17A、CXCL10表达均呈负相关(r=-0.006、r=-0.637,P0.05),IL-17A与CXCL10表达呈正相关(r=0.896,P0.05)。结论:1.哮喘缓解期气道炎症可能与IL-17A、IL-10及CXCL10水平的改变有关。2.固本防哮饮对IL-17A、IL-10及CXCL10的表达有一定的调节作用。3.固本防哮饮治疗哮喘的作用机制可能是通过上调IL-10水平,降低IL-17A、CXCL10水平,减轻气道炎性反应,从而达到防治哮喘的目的。
[Abstract]:Objective: asthma is a chronic inflammatory respiratory disease, which is a serious threat to human health. The incidence and mortality of asthma are increasing year by year. The traditional Chinese medicine prescription "Ben Ben Wei Xiao" is a famous Chinese pediatrics expert, Professor Jiang Yuren. It has been used for many years, and its curative effect is definite. It can enhance the physical fitness of children, prevent asthma attacks, improve the quality of life and long-term remission rate of asthma. In the field of biomedicine, it has been clear that interleukin -17A (IL-17A), interleukin -10 (IL-10) and CXC chemokine ligand 10 (CXCL10) are closely related to asthma. In this study, we adopted the animal model of asthma remission stage to study the effect of Chinese herbal medicine "Ben Ben Xiao Xiao Yin" on the expression of IL-17A, IL-10 and CXCL10 in asthmatic mice, and to observe its effect on airway inflammation in asthmatic remission model mice and the mechanism of preventing and treating asthma. Methods: using ovalbumin (OVA) sensitization and respiratory syncytial virus (RSV) nasal induced BALB/c female mice to establish animal model of asthma, were randomly divided into normal group, model group, gbfxd low, medium and high dose group, montelukast sodium group and dexamethasone pine group. The general behavioral activities of mice were observed. The expression levels of IL-17A, IL-10 and CXCL10 protein in lung tissue were detected by ELISA. The expression levels of IL-17A, IL-10 and CXCL10mRNA in lung tissue were detected by Real-timePCR. Finally, the statistical method is used to analyze and deal with it. Results: IL-17A were significantly higher than the normal group, the expression of CXCL10 in lung tissue of mice in the model group (P0.01), and the expression of IL-10 was significantly lower than that of normal group (P0.01); gbfxd low, medium and high dose group were lower than the model group, the expression of CXCL10 IL-17A Meng Lu Lester sodium and dexamethasone in lung tissue of mice in (P0.05), and the expression of IL-10 is higher than that of the model group (P0.05). Correlation analysis showed that IL-10 expression was negatively correlated with IL-17A and CXCL10 expression in model group mice (r=-0.006, r=-0.637, P0.05), and IL-17A was positively correlated with CXCL10 expression (r=0.896, P0.05) in ELISA detection. Conclusion: 1. airway inflammation in the remission period of asthma may be associated with changes in the levels of IL-17A, IL-10 and CXCL10. 2. the expression of IL-17A, IL-10 and CXCL10 had a certain regulating effect. 3., the effective mechanism of "Gu Ben Xiao Xiao Yin" in treating asthma is probably by increasing the level of IL-10, decreasing the level of IL-17A and CXCL10, and reducing the inflammatory reaction of airway, so as to achieve the purpose of preventing and treating asthma.
【学位授予单位】：南京中医药大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R285.5;R-332

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