短期饮用某酱香型白酒对大鼠乙醇代谢的影响

发布时间：2017-12-28 09:07

本文关键词：短期饮用某酱香型白酒对大鼠乙醇代谢的影响　出处：《广东医学》2013年24期 　论文类型：期刊论文

【摘要】：目的观察高、中、低不同剂量的某酱香型白酒对大鼠乙醇脱氢酶(ADH)、乙醛脱氢酶(ALDH)含量的影响,以探讨该酒与体内乙醇代谢的关系。方法 75只雄性Wistar大鼠随机分为空白对照组,乙醇组,白酒高、中、低剂量组,分别给予大鼠灌胃生理盐水5 mL/kg,乙醇5 mL/kg,某酱香型白酒10、5、2.5 mL/kg,1次/d,连续7 d。末次灌胃12 h后处死大鼠,检测大鼠血中乙醇、乙醛浓度,肝组织匀浆中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-P X)、丙二醛(MDA)、ADH、ALDH含量,RT-PCR、免疫组织化学法和Western blot检测大鼠肝组织中ADH1、ALDH2 mRNA及蛋白质的表达。结果与乙醇组比较,末次灌胃12 h后白酒中、低剂量组大鼠血中乙醇、乙醛浓度明显降低(P0.01);在大鼠肝匀浆中,白酒高、中、低剂量组SOD、GSH-P X含量均明显升高(P0.01),白酒中、低剂量组MDA含量明显降低(P0.01),白酒低剂量组ADH含量明显升高(P0.01),白酒高、中、低剂量组ALDH含量均明显升高(P0.01);肝组织中,白酒低剂量组ADH1 mRNA及蛋白质表达明显升高(P0.01),白酒高、中、低剂量组ALDH2 mRNA及蛋白质表达均明显升高(P0.01)。结论该酱香型白酒对大鼠ADH的影响与乙醇无明显差异,但可明显升高大鼠ALDH活性,从而减少乙醛在体内的蓄积,减轻乙醛对机体的毒害作用。
[Abstract]:Objective To observe the effects of a high, medium and low dose of a sauce flavor liquor on the contents of alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH) in rats, so as to explore the relationship between alcohol and the metabolism of alcohol in vivo. Methods 75 male Wistar rats were randomly divided into control group, ethanol group, liquor high, medium and low dose group, the rats were given normal saline 5 mL/kg, ethanol 5 mL/kg, a Maotai liquor 10, 5, 2.5 mL/kg, 1 /d, 7 consecutive D. At the end of gavage. The rats were sacrificed 12 h after ethanol and acetaldehyde concentration in rat serum, liver homogenate superoxide dismutase (SOD), glutathione peroxidase (GSH-P X) and malondialdehyde (MDA), ADH, ALDH content, expression of RT-PCR, immunohistochemistry and Western blot assay in rats liver ADH1, ALDH2 and mRNA protein. Results compared with the alcohol group, 12 h after the last administration of liquor in the low dose group rat blood ethanol and acetaldehyde concentrations were significantly decreased (P0.01); in rat liver homogenate, liquor high, medium and low dose group SOD, GSH-P and X were obviously increased (P0.01), and low in spirits dose group MDA decreased significantly (P0.01), the liquor content of ADH was significantly increased in low dose group (P0.01), the liquor is high, medium and low dose group ALDH content were significantly increased (P0.01); liver tissue, expression of liquor and low dose groups of ADH1 mRNA and protein were significantly increased (P0.01), high, middle and low liquor the dose group of ALDH2 mRNA and protein expression were significantly increased (P0.01). No significant differences in the conclusions of Maotai liquor effect on ADH in rats with ethanol, but can significantly increase ALDH activity in rats, thereby reducing the acetaldehyde accumulation in the body, reduce the toxic effects of acetaldehyde on the body.
【作者单位】：贵阳医学院附属医院感染科;
【基金】：国家重点基础研究发展计划(973)课题(编号:2011CB512114) 贵州省教育厅自然科学研究项目(编号:黔教科[2011]037号)
【分类号】：R363
【正文快照】： 酒在人类社交活动中起着至关重要的作用,但过量饮酒可导致全身各脏器受损,如脑、心血管系统、胃肠道等,尤以肝脏受损最为突出,过度饮酒导致的死亡人数占全球人口死亡的3.8%[1-5]。在中国,肝脏疾病以肝炎病毒感染多见,其次,酒精性肝病(alcoholic liverdisease)的发生率有增加

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