基于小鼠Morris水迷宫和Y迷宫联合试验的多任务行为学测试研究

发布时间：2017-12-31 05:14

本文关键词：基于小鼠Morris水迷宫和Y迷宫联合试验的多任务行为学测试研究　出处：《神经解剖学杂志》2013年04期 　论文类型：期刊论文

【摘要】：目的:通过观察小鼠Morris水迷宫和Y迷宫多任务联合试验与单独水迷宫或Y迷宫试验的结果差异,探讨两种记忆相关行为学试验之间是否存在相互干扰。方法:30只成年昆明小鼠随机分为单独水迷宫、单独Y迷宫和水迷宫+Y迷宫(简称联合组)三组。水迷宫试验记录小鼠寻找水下平台的逃避潜伏期、逃避距离、撤除平台后在目标象限内游泳时间和距离占全部时间与距离的百分比;Y迷宫试验记录小鼠在光-电刺激下逃往安全区的错误次数。结果:联合组与单独水迷宫组相比,小鼠逃避潜伏期、逃避距离以及目标象限内游泳所占的时间和距离百分比均无明显差异(P0.05);联合组与单独Y迷宫组相比,第1和第2 d两组小鼠到达安全区的错误次数无明显差异,第3和第4 d联合组较单独组错误次数明显减少(P0.05)。结论:两种行为学联合序贯试验对小鼠水迷宫空间学习记忆及第1至2 d Y迷宫工作记忆均不产生干扰;超过2 d的联合实验有助于增强小鼠Y迷宫工作记忆。因此,本研究为联合开展小鼠空间记忆和工作记忆研究提供了实验依据,提示合理地联合不同行为学实验将有助于提高实验效率、降低实验成本。
[Abstract]:Objective: to observe the results of Morris water maze and Y maze multitasking test in mice and single water maze or Y maze test. Methods 30 adult Kunming mice were randomly divided into water maze. Single Y maze and water maze Y maze (combined group). The water maze test recorded the escape latency and distance of mice looking for underwater platform. The percentage of swimming time and distance in the target quadrant after the removal of the platform; Y-maze test recorded the wrong times of mice escaping to the safety zone under photo-electric stimulation. Results: compared with the water maze group, the escape latency of the combined group was higher than that of the single water maze group. There was no significant difference in escape distance, time and distance percentage of swimming in target quadrant (P 0.05). There was no significant difference in the number of errors to the safety zone between the combined group and the Y-maze group on the 1st and 2nd days. The number of errors in the 3rd and 4th day group was significantly lower than that in the single group (P0.05). Conclusion: both behavioral and sequential tests did not interfere with the spatial learning and memory of water maze and the working memory of 1 to 2 d Y maze in mice. The combined experiment for more than 2 days was helpful to enhance the working memory of Y maze in mice. Therefore, this study provided the experimental basis for the joint study of spatial memory and working memory in mice. It is suggested that reasonable combination of different behavioral experiments will help to improve the experimental efficiency and reduce the experimental cost.
【作者单位】：山西医科大学生理学系细胞生理学省部共建教育部重点实验室;
【基金】：国家自然科学基金(31271201) 教育部高等学校博士学科点专项科研基金(博导类,20101417110001) 山西省自然科学基金(2010011049-3)
【分类号】：R-332
【正文快照】： 水迷宫是科学家R.G.M.Morris于20世纪80年代首次提出的用于研究啮齿类动物学习记忆的经典行为学实验方法[1]。由于啮齿类动物可以依据Morris水迷宫空间内不同的标志物寻找到隐藏在迷宫水面下方用于逃生的安全平台,因此Morris水迷宫可用于检测动物的空间学习和记忆功能。Y迷宫

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