良附丸对实验性胃溃疡模型大鼠的药效学研究

发布时间：2018-01-02 05:02

本文关键词：良附丸对实验性胃溃疡模型大鼠的药效学研究　出处：《黑龙江中医药大学》2017年硕士论文　论文类型：学位论文

【摘要】：目的:本实验建立四种实验性胃溃疡动物模型,观察良附丸对模型动物的防治作用,并明确其防治效果;进而观察良附丸各剂量组对实验性胃溃疡的药效,并初步探讨良附丸抗实验性胃溃疡的作用机制。方法:实验分两个阶段进行,第一阶段明确良附丸对四种实验性胃溃疡的防治效果及良附丸各剂量组对实验性胃溃疡的药效。四种实验性胃溃疡模型分别为幽门结扎型、束缚-水浸应激型、消炎痛型及醋酸涂抹型。第一步将每种模型分为3组:良附丸组、模型组、阳性对照组,各组大鼠分别进行实验、取材,观察胃组织病理变化,测量溃疡指数并计算溃疡愈合率;根据第一步实验所得结果,第二步探讨良附丸各剂量抗束缚-水浸应激型胃溃疡的药效,分为6组,良附丸高、中、低剂量组,模型组,空白对照组,阳性对照组,实验步骤同第一步。根据第一阶段所得实验结果,第二阶段探讨良附丸对束缚-水浸应激型胃溃疡的作用机制,分为4组:空白组、良附丸组、模型组、阳性对照组,预防性给药4天后造模,腹主动脉取血、取下丘脑、取胃,观察胃组织损伤情况,测量溃疡指数计算溃疡愈合率,测定大鼠血清中SOD、MDA、NO和脑组织中5-HT的含量。结果:良附丸对幽门结扎型和束缚-水浸应激型胃溃疡均有防治作用,能使胃黏膜损伤程度降低,溃疡抑制率升高,且对束缚-水浸应激型胃溃疡抑制率高于幽门结扎型胃溃疡。良附丸高剂量组及阳性对照组可显著降低溃疡指数(p0.01),且效果优于中、低剂量组。良附丸高剂量组较模型组相比,能够升高大鼠血清中NO、SOD,降低血清中MDA及脑组织中5-HT含量(p0.05,p0.01)。结论:1.良附丸对幽门结扎型和束缚-水浸应激型胃溃疡均有防治作用。2.束缚-水浸应激型胃溃疡:良附丸高、中剂量组对束缚-水浸应激型胃溃疡均有防治作用,且高剂量组的防治效果优于其他剂量组。3.良附丸促束缚-水浸应激型大鼠胃溃疡愈合的作用机制可能通过升高大鼠血清中保护因子SOD、NO的含量,降低血清MDA及脑组织5-HT含量来实现的。
[Abstract]:Objective: to establish four animal models of experimental gastric ulcer and observe the preventive and therapeutic effects of Liangfu pills on model animals. Then observe the effect of Liangfu Pill on experimental gastric ulcer and explore the mechanism of Liangfu Pill on experimental gastric ulcer. Methods: the experiment was carried out in two stages. In the first stage, the preventive and therapeutic effects of Liangfu Pill on four kinds of experimental gastric ulcer were clarified. The four experimental gastric ulcer models were pyloric ligation type and irreducible water-immersion stress type. In the first step, each model was divided into three groups: Liangfu pill group, model group, positive control group, each group of rats were experimented, taken materials, observed the pathological changes of gastric tissue. Ulcer index was measured and ulcer healing rate was calculated. According to the results of the first step, the second step was to investigate the efficacy of Liangfu Pill in anti-restraint and water-immersion stress type gastric ulcer. It was divided into 6 groups: high, middle and low dose group, model group and blank control group. According to the experimental results obtained in the first stage, the mechanism of Liangfu Pill on irreducible water-immersion stress gastric ulcer was studied in the second stage, and was divided into four groups: blank group and Liangfu Pill group. Model group, positive control group, preventive administration 4 days after the model, abdominal aorta blood, hypothalamus, stomach, observe gastric tissue injury, ulcer index to calculate ulcer healing rate. The content of 5-HT in brain tissue and serum SOD MDADAN were determined. Results: Liangfu pills could prevent and treat gastric ulcer of pyloric ligation type and irreducible water-immersion stress type. It can reduce the degree of gastric mucosal injury and increase the inhibition rate of ulcer. The inhibition rate of gastric ulcer in the restraint water immersion stress type was higher than that in the pyloric ligation type. The high dose group of Liangfu pills and the positive control group could significantly reduce the ulcer index (P 0.01), and the effect was better than that in the middle group. Compared with the model group, the high dose group of Liangfu pills could increase the serum NON-SOD, decrease the MDA in serum and 5-HT content in brain tissue. Conclusion: Liangfu Pill can prevent and cure gastric ulcer of pyloric ligation type and bound water stress type. 2. Restraint and water immersion stress type gastric ulcer: Liangfu Pill is high. The middle dose group had the preventive and therapeutic effect on the gastric ulcer induced by irreducible water immersion stress. The preventive and therapeutic effect of high dose group was better than that of other dosage group. 3. The mechanism of Liangfu pills promoting the healing of gastric ulcer induced by water immersion stress might be by increasing the content of protective factor SODN in the serum of rats. To reduce serum MDA and brain tissue 5-HT content to achieve.
【学位授予单位】：黑龙江中医药大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R285.5;R-332

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