核酸疫苗初免-蛋白疫苗加强的免疫策略提高日本血吸虫核酸疫苗免疫效果

发布时间：2018-01-09 01:14

本文关键词：核酸疫苗初免-蛋白疫苗加强的免疫策略提高日本血吸虫核酸疫苗免疫效果　出处：《生物工程学报》2013年06期 　论文类型：期刊论文

【摘要】：为研制抗血吸虫疫苗提供实验依据,探讨了抗血吸虫SjGST-32核酸疫苗与蛋白疫苗联合免疫的免疫增强效应及免疫应答特征。将日本血吸虫DNA疫苗VR1012-SjGST-32与重组蛋白疫苗rSjGST-32分别在第0、2和4周免疫小鼠,在第6周攻击感染日本血吸虫尾蚴,攻击感染45 d后剖杀小鼠,计算减虫率、检卵率以及检测肝脏病理变化,观察免疫保护效果;检测小鼠血清中特异性IgG抗体滴度,T细胞增殖反应和抗原特异性CD4+IFN-γ+、CD4+IL-4+和CD4+IL-10+的数量,探讨免疫应答特征。结果显示,DNA初免-蛋白加强的联合免疫组的保护作用优于单独免疫组,显著提高了减虫率(42.3%)和减卵率(59.6%),并且能够显著减轻血吸虫虫卵对肝脏的病理损害;进一步发现,DNA疫苗和蛋白疫苗联合应用增强了机体T淋巴细胞增殖反应、抗体IgG滴度以及抗原特异性CD4+IFN-γ+的产生。这些研究为新型血吸虫疫苗的优化设计和合理应用提供了依据。
[Abstract]:To provide the experimental basis for the development of anti schistosome vaccine, discusses the immunity against Schistosoma japonicum SjGST-32 DNA vaccine and protein vaccine combined with immune enhancement effect and immune response characteristics. The DNA vaccine of Schistosoma japonicum VR1012-SjGST-32 vaccine and recombinant protein rSjGST-32 respectively in 0,2 and 4 weeks of mice infected with Schistosoma japonicum cercariae attack in sixth weeks, 45 D infection after the mice were sacrificed and the calculation of the worm reduction rate, detection rate and detection of egg liver pathological changes, observe the immune protective effect; titer of specific IgG antibody in the serum of mice, T cells proliferation and antigen specific CD4+IFN- +, the number of CD4+IL-4+ and CD4+IL-10+, to explore the characteristics of immune response. The results show that the effect is better than that of single the immune protection group combined with a DNA prime protein enhanced, significantly improve the worm reduction rate (42.3%) and egg reduction rate (59.6%), and can significantly reduce schistosomiasis Egg pathological damage to the liver; further found that DNA vaccine and protein vaccine combined with enhanced body T lymphocyte proliferation, IgG antibody titer and antigen specific CD4+IFN- + gamma production. These studies provide a basis for optimizing the design of a new type of schistosome vaccine and reasonable application.

【作者单位】：内蒙古大学生命科学学院;湖南省血吸虫病防治研究所;
【基金】：农业生物技术国家重点实验室开放课题(No.2008SKLAB08-01)资助~~
【分类号】：R392
【正文快照】： 日本血吸虫病是一种流行广泛、危害严重的人兽共患寄生虫病。如何有效控制和预防日本血吸虫成为全世界普遍关注的公共卫生问题。研制血吸虫病疫苗可作为化疗的补充,能更有效地控制血吸虫感染。近年来己经开展了血吸虫DNA疫苗的研制,并且取得了一定的效果,但其免疫保护效果还

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