高原缺氧对巨噬细胞CD14、CD64、MHC-Ⅱ类分子表达及细胞因子释放的影响

发布时间：2018-01-15 02:25

  本文关键词：高原缺氧对巨噬细胞CD14、CD64、MHC-Ⅱ类分子表达及细胞因子释放的影响　出处：《青海大学》2017年硕士论文　论文类型：学位论文

  更多相关文章： 低氧 Mφ 吞噬能力 NO 表面分子 IL-6 TNF-α

【摘要】：目的:通过建立高原低氧环境的小鼠模型,探索低氧条件如何影响机体固有免疫细胞-巨噬细胞(Mφ)的吞噬杀伤功能、表面分子表达以及分泌IL-6、TNF-α功能的影响。方法:分别在400 m,2200 m和4200 m的不同海拔条件下暴露小鼠30d后,(1)用流式细胞仪检测小鼠腹腔Mφ对FITC标记金黄色葡萄球菌的吞噬能力;(2)用双乙酰基二氯荧光素(DCFH-DA)荧光探针法检测Mφ呼吸爆发功能;(3)用ELISA试剂盒检测小鼠Mφ培养上清中的NO稳定氧化代谢产物亚硝酸盐(NO2-)水平;(4)用流式细胞术(Flow cytometry,FCM)分析法观察其腹腔Mφ表面分子的变化;(5)用ELISA试剂盒检测小鼠腹腔Mφ培养上清中的IL-6、TNF-a的浓度。结果:海拔4200m和海拔2200m低氧暴露30d后,Mφ吞噬能力、呼吸爆发能力及NO释放量降低;小鼠腹腔MφCD14、CD64、TLR4、CD11b、CD18、CD80、MHC-Ⅱ分子的表达量较对照组(海拔400 m)均显著下降(P0.05)。此外,海拔4200 m低氧组与海拔2200 m低氧组相比,小鼠腹腔MφCD14、CD64、TLR4、CD11b、CD18、CD80、MHC-Ⅱ分子的表达量均明显下降(P0.05);海拔4200 m和2200 m实验组小鼠与对照组(海拔400m)相比,培养上清中的IL-6、TNF-a的浓度MφIL-6、TNF-α的浓度明显升高(P0.05)。结论:海拔4200 m和2200 m高原低氧暴露30d后,Mφ的吞噬能力和杀伤能力降低;小鼠腹腔Mφ表面分子表达水平各自发生显著改变;小鼠腹腔Mφ分泌细胞因子的能力发生改变。说明高原低氧在一定程度上抑制Mφ吞噬杀伤功能,促使Mφ的炎性反应及改变Mφ的免疫调节能力。
[Abstract]:Objective: to investigate the effects of hypoxia on phagocytosis, expression of surface molecules and secretion of IL-6 in innate immune cell-macrophage M 蠁 by establishing a mouse model of high altitude hypoxia. Effects of TNF- 伪 on the function of mice. Methods: mice were exposed to TNF- 伪 for 30 days at different altitudes of 400 m, 2200m and 4200m, respectively. 1) the phagocytosis of M 蠁 on FITC labeled Staphylococcus aureus was detected by flow cytometry. (2) the respiratory burst function of M 蠁 was detected by diacetyldichlorofluorescein (DCFH-DA) fluorescence probe method. (3) ELISA kit was used to detect the no stable oxidation metabolite (nitrite) in the culture supernatant of M 蠁 of mice. (4) flow cytometric (FCM) analysis was used to observe the changes of M 蠁 surface molecules in abdominal cavity. ELISA kit was used to detect the concentration of IL-6 and TNF-a in mouse peritoneal M 蠁 culture supernatant. Results: after exposure to hypoxia at altitude of 4200m and 2200m for 30 days. M 蠁 phagocytosis, respiratory burst and no release decreased. In mice, M 蠁 CD14, CD64, TLR4, CD11b, CD18, CD80. The expression of MHC- 鈪，

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