压应力对小鼠单核细胞RAW264.7 DNAX活化蛋白12、抗酒石酸酸性磷酸酶表达的影响

发布时间：2018-01-16 03:02

本文关键词：压应力对小鼠单核细胞RAW264.7 DNAX活化蛋白12、抗酒石酸酸性磷酸酶表达的影响　出处：《华西口腔医学杂志》2013年04期 　论文类型：期刊论文

【摘要】：目的探讨压应力对小鼠单核细胞RAW264.7 DNAX活化蛋白12(DAP12)、抗酒石酸酸性磷酸酶(TRAP)表达的影响。方法以小鼠单核细胞RAW264.7为研究对象,采用四点弯曲体外细胞加载装置加载压应力0、3、6、12h,分别以逆转录聚合酶链反应(RT-PCR)、蛋白免疫印迹法检测DAP12、TRAP mRNA和DAP12蛋白的表达情况。结果小鼠单核细胞RAW264.7经破骨细胞培养液培养后,体积变大,核数目增多,TRAP染色阳性。受压应力刺激后,DAP12、TRAP mRNA及DAP12蛋白表达随加力时间延长而增加(P0.05)。结论小鼠单核细胞RAW264.7经破骨细胞培养液培养后成功向破骨细胞转化,转化细胞受压应力刺激活化过程中存在DAP12高表达。
[Abstract]:Objective to investigate the effect of compressive stress on RAW264.7 DNAX activator protein 12 (DAP12) in mouse monocytes. Methods RAW264.7 of mouse monocytes was studied by using a four-point bending cell loading device in vitro. At 12 h, DAP12 was detected by reverse transcriptase polymerase chain reaction (RT-PCR) and Western blot respectively. The expression of TRAP mRNA and DAP12 protein. Results RAW264.7 of mouse monocytes cultured in osteoclast culture medium increased in volume and number of nuclei. TRAP staining was positive. DAP12 was stimulated by compressive stress. The expression of TRAP mRNA and DAP12 protein increased with the extension of stress time (P0.05). Conclusion Mouse monocyte RAW264.7 was successfully transformed into osteoclast after cultured in osteoclast culture medium. There was high expression of DAP12 during the activation of transformed cells stimulated by compressive stress.
【作者单位】：中南大学湘雅二医院口腔中心;
【分类号】：R363
【正文快照】： 破骨细胞由其前体细胞分化而来,在其分化并行使功能的过程中受多条信号传导通路调控。其中免疫受体酪氨酸活化基序(immunoreceptor tyrosine-based activation motif,ITAM)信号传导通路日益受到人们的重视。已知DNAX活化蛋白12(DNAX-activa-ting protein 12,DAP12)是骨髓来源

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